Functional analysis of nuclear envelope targeting plasma membrane Proteins : using EGF family proteins

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: HIEDA Miki 大阪大学, 大学院・医学系研究科, 特任助教(常勤) (00380254)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Hiroshi 大阪大学, 大学院・生命機能研究科, 准教授 (30241392)
• Project Period (FY): 2009 – 2011
• Keywords: 細胞構造・機能 / 細胞核 / 転写調節

Research Abstract

An EGF family member, amphiregulin, which has been suggested to play role(s) in breast cancer, is expressed on the cell surface in pro-form. Shedding stimuli result in release of soluble EGFR ligand and migration of the pro-form to the nuclear envelope. This study demonstrates that nuclear envelope-localized proAREG activates breast cancer cell migration and the activation of cell migration does not require EGFR function, but does occur concomitantly with an alteration of histone modification.

Research Products

• [Journal Article] Nuclear envelope-localized EGF family protein amphiregulin activates breast cancer cell migration in an EGF-like domain independent manner (2012)
  • Author(s): Tanaka, H., Nishioka, Y., Yokoyama Y., Higashiyama S., Matsuura, N., Matsuura, S., Hieda, M.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 420 Pages: 721-726
  • DOI: DOI:10.1016/j.bbrc.2012.03.045
  • Peer Reviewed
• [Journal Article] Breaking the HAC Barrier : Histone H3K9 acetyl/ methyl balance regulates CENP-A assembly (2012)
  • Author(s): Ohzeki J-i, Bergmann JH, Kouprina N, Noskov VN, Nakano M, Kimura H, Earnshaw WC, Larionov V, and Masumoto H
  • Journal Title: EMBO J Volume: (in press)
  • DOI: DOI:10.1038/emboj.2012.82
  • Peer Reviewed
• [Journal Article] Transition of Chromatin Status During The Recovery Process from Drought Stress in Arabidopsis thaliana (2012)
  • Author(s): Kim J-M, To TK, Ishida J, Matsui A, Kimura H, Seki M
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 847-856
  • DOI: DOI:10.1093/pcp/pcs053
  • Peer Reviewed
• [Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis (2012)
  • Author(s): Ushijima Y, Inoue YH, Konishi T, Kitazawa D, Yoshida H, Shimaji K, Kimura H, and Yamaguchi M
  • Journal Title: Chromosome Res Volume: 20 Pages: 319-331
  • DOI: DOI:10.1007/s10577-012-9276-1
  • Peer Reviewed
• [Journal Article] Fluorescence Cell Imaging and Manipulation Using Conventional Halogen Lamp Microscopy (2012)
  • Author(s): Yamagata K, Iwamoto D, Terashita Y, Li C, Wakayama S, Hayashi-Takanaka Y, Kimura H, Saeki K, and Wakayama T
  • Journal Title: PLoS ONE Volume: 7 Pages: e31638
  • DOI: DOI:10.1371/journal.pone.0031638
  • Peer Reviewed
• [Journal Article] Epigenetic Engineering : Histone H3K9 acetylation is compatible with kinetochore structure and function (2012)
  • Author(s): Bergmann JH, Jakubsche JN, Martins NM, Kagansky A, Nakano M, Kimura H, Kelly DA, Turner BM, Masumoto H, Larionov V, and Earnshaw WC
  • Journal Title: J Cell Sci Volume: 125 Pages: 411-421
  • DOI: DOI:10.1242/jcs.090639
  • Peer Reviewed
• [Journal Article] Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant (2012)
  • Author(s): Brookes E, de Santiago I, Hebenstreit D, Morris KJ, Carroll T, Xie SQ, Stock JK, Heidemann M, Eick D, Nozaki N, Kimura H, Ragoussis J, Teichmann SA, and Pombo A
  • Journal Title: and Regulates Metabolic Genes in ESCs. Cell Stem Cell Volume: 10 Pages: 157-170
  • DOI: DOI:10.1016/j.stem.2011.12.017
  • Peer Reviewed
• [Journal Article] Platelet-Derived Growth Factor Regulates Breast Cancer Progression via・-Catenin Expression (2011)
  • Author(s): Yokoyama, Y., Mori, S., Hamada, Y., Hieda, M., Kawaguchi, N., Shaker, M., Tao, Y., Yoshidome, K., Tsujimoto, M., Matsuura, N.
  • Journal Title: Pathobiology Volume: 78 Pages: 253-260
  • DOI: DOI:10.1159/000328061
  • Peer Reviewed
• [Journal Article] Submembranous septins as relatively stable components of actin-based membrane skeleton (2011)
  • Author(s): Hagiwara A, Tanaka Y, Hikawa R, Morone N, Kusumi A, Kimura H, and Kinoshita M
  • Journal Title: Cytoskeleton(Hoboken) Volume: 68 Pages: 512-525
  • DOI: DOI:10.1002/cm.20528
  • Peer Reviewed
• [Journal Article] Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling (2011)
  • Author(s): Hayashi-Takanaka Y, Yamagata K, Wakayama T, Stasevich TJ, Kainuma T, Tsurimoto T, Tachibana M, Shinkai Y, Kurumizaka H, Nozaki N, and Kimura H
  • Journal Title: Nucleic Acids Res Volume: 39 Pages: 6475-6488
  • DOI: DOI:10.1093/nar/gkr343
  • Peer Reviewed
• [Journal Article] Crystal structure of the human centromeric nucleosome containing CENP-A (2011)
  • Author(s): Tachiwana H, Kagawa W, Shiga T, Osakabe A, Miya Y, Saito K, Hayashi-Takanaka Y, Oda T, Sato M, Park SY, Kimura H, and Kurumizaka H
  • Journal Title: Nature Volume: 476 Pages: 232-235
  • DOI: DOI:10.1038/nature10258
  • Peer Reviewed
• [Journal Article] Structures of human nucleosomes containing major histone H3 variants (2011)
  • Author(s): Tachiwana H, Osakabe A, Shiga T, Miya Y, Kimura H, Kagawa W, and Kurumizaka H
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 67 Pages: 578-583
  • DOI: DOI:10.1107/S0907444911014818
  • Peer Reviewed
• [Journal Article] Enhancement of myosin II/ actin turnover at the contractile ring induces slower furrowing in dividing HeLa cells (2011)
  • Author(s): Kondo T, Hamao K, Kamijo K, Kimura H, Morita M, Takahashi M, and Hosoya H
  • Journal Title: Biochem J Volume: 435 Pages: 569-576
  • DOI: DOI:10.1042/BJ20100837
  • Peer Reviewed
• [Journal Article] Role of claudin species-specific dynamics in reconstitution and remodeling of the zonula occludens (2011)
  • Author(s): Yamazaki Y, Tokumasu R, Kimura H, and Tsukita S
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 1495-1504
  • DOI: DOI:10.1091/mbc.E10-12-1003
  • Peer Reviewed
• [Journal Article] Arabidopsis HDA6 regulates locus-directed heterochromatin silencing in cooperation with MET1 (2011)
  • Author(s): To TK, Kim JM, Matsui A, Kurihara Y, Morosawa T, Ishida J, Tanaka M, Endo T, Kakutani T, Toyoda T, Kimura H, Yokoyama S, Shinozaki K, and Seki M
  • Journal Title: PLoS Genet Volume: 7 Pages: e1002055
  • DOI: DOI:10.1371/journal.pgen.1002055
  • Peer Reviewed
• [Journal Article] Broad chromosomal domains of histone modification patterns in C (2011)
  • Author(s): Liu T, Rechtsteiner A, Egelhofer TA, Vielle A, Latorre I, Cheung MS, Ercan S, Ikegami K, Jensen M, Kolasinska-Zwierz P, Rosenbaum H, Shin H, Taing S, Takasaki T, Iniguez AL, Desai A, Dernburg AF, Kimura H, Lieb JD, Ahringer J, Strome S, and Liu XS
  • Journal Title: elegans. Genome Res Volume: 21 Pages: 227-236
  • DOI: DOI:10.1101/gr.115519.110
  • Peer Reviewed
• [Journal Article] Epigenetic engineering shows H3K4me2 is required for HJURP targeting and CENP-A assembly on a synthetic human kinetochore (2011)
  • Author(s): Bergmann JH, Rodriguez MG, Martins NM, Kimura H, Kelly DA, Masumoto H, Larionov V, Jansen LE, and Earnshaw WC
  • Journal Title: EMBO J Volume: 30 Pages: 328-340
  • DOI: DOI:10.1038/emboj.2010.329
  • Peer Reviewed
• [Journal Article] Autocatalytic differentiation of epigenetic modifications within the Arabidopsis genome (2010)
  • Author(s): Inagaki S, Miura-Kamio A, Nakamura Y, Lu F, Cui X, Cao X, Kimura H, Saze H, and Kakutani T
  • Journal Title: EMBO J Volume: 29 Pages: 3496-3506
  • DOI: DOI:10.1038/emboj.2010.227
  • Peer Reviewed
• [Journal Article] Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B (2010)
  • Author(s): Kelly AE, Ghenoiu C, Xue JZ, Zierhut C, Kimura H, and Funabiki H
  • Journal Title: Science Volume: 330 Pages: 235-239
  • DOI: DOI:10.1126/science.1189505
  • Peer Reviewed
• [Journal Article] The histone H3K36 methyltransferase MES-4 acts epigenetically to transmit the memory of germline gene expression to progeny (2010)
  • Author(s): Rechtsteiner A, Ercan S, Takasaki T, Phippen TM, Egelhofer TA, Wang W, Kimura H, Lieb JD, and Strome S
  • Journal Title: PLoS Genet Volume: 6 Pages: e1001091
  • DOI: DOI:10.1371/journal.pgen.1001091
  • Peer Reviewed
• [Journal Article] Oncogenesis by sequestration of CBP/ p300 in transcriptionally inactive hyperacetylated chromatin domains (2010)
  • Author(s): Reynoird N, Schwartz BE, Delvecchio M, Sadoul K, Meyers D, Mukherjee C, Caron C, Kimura H, Rousseaux S, Cole PA, Panne D, French CA, and Khochbin S
  • Journal Title: EMBO J. Volume: 29 Pages: 2943-2952
  • DOI: DOI:10.1038/emboj.2010.176
  • Peer Reviewed
• [Journal Article] Trans-generational epigenetic regulation of C. elegans primordial germ cells (2010)
  • Author(s): Furuhashi H, Takasaki T, Rechtsteiner A, Li T, Kimura H, Checchi PM, Strome S, and Kelly WG
  • Journal Title: Epigenetics Chromatin Volume: 3 Pages: 15
  • DOI: DOI:10.1186/1756-8935-3-15
  • Peer Reviewed
• [Journal Article] Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation (2010)
  • Author(s): Nozawa RS, Nagao K, Masuda HT, Iwasaki O, Hirota T, Nozaki N, Kimura H, Obuse C
  • Journal Title: Nat Cell Biol Volume: 12 Pages: 719-727
  • DOI: DOI:10.1038/ncb2075
  • Peer Reviewed
• [Journal Article] Structural basis of instability of the nucleosome containing a testis-specific histone variant, human H3T (2010)
  • Author(s): Tachiwana H, Kagawa W, Osakabe A, Kawaguchi K, Shiga T, Hayashi-Takanaka Y, Kimura H, and Kurumizaka H
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Pages: 10454-10459
  • DOI: DOI:10.1073/pnas.1003064107
  • Peer Reviewed
• [Journal Article] Histone H1 null vertebrate cells exhibit altered nucleosome architecture (2010)
  • Author(s): Hashimoto H, Takami Y, Sonoda E, Iwasaki T, Iwano H, Tachibana M, Takeda S, Nakayama T, Kimura H, and Shinkai Y
  • Journal Title: Nucleic Acids Res Volume: 38 Pages: 3533-3545
  • DOI: DOI:10.1093/nar/gkq076
  • Peer Reviewed
• [Journal Article] Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET (2010)
  • Author(s): Matsui T, Leung D, Miyashita H, Maksakova IA, Miyachi H, Kimura H, Tachibana M, Lorincz MC, and Shinkai Y
  • Journal Title: Nature Volume: 464 Pages: 927-931
  • DOI: DOI:10.1038/nature08858
  • Peer Reviewed
• [Journal Article] Transduction of RNA-directed DNA methylation signals to repressive histone marks in Arabidopsis thaliana (2010)
  • Author(s): Numa H, Kim JM, Matsui A, Kurihara Y, Morosawa T, Ishida J, Mochizuki Y, Kimura H, Shinozaki K, Toyoda T, Seki M, Yoshikawa M, and Habu Y
  • Journal Title: EMBO J Volume: 29 Pages: 352-362
  • DOI: DOI:10.1038/emboj.2009.374
  • Peer Reviewed
• [Journal Article] Visualizing histone modifications in living cells : spatiotemporal dynamics of H3 phosphorylation during interphase (2009)
  • Author(s): Hayashi-Takanaka Y, Yamagata K, Nozaki N, and Kimura H
  • Journal Title: J Cell Biol Volume: 187 Pages: 781-790
  • DOI: DOI:10.1083/jcb.200904137
  • Peer Reviewed
• [Journal Article] Inactive X chromosome-specific histone H3 modifications and CpG hypomethylation flank a chromatin boundary between an X-inactivated and an escape gene (2009)
  • Author(s): Goto Y and Kimura H
  • Journal Title: Nucleic Acids Res Volume: 37 Pages: 7416-7428
  • DOI: DOI:10.1093/nar/gkp860
  • Peer Reviewed
• [Presentation] in vitro核膜内膜タンパク質局在化assay系の構築、(Development of an in vitro nuclear membrane protein targeting system) (2011)
  • Author(s): 野村菜美子、西岡優、松浦成昭、松浦脩治、檜枝美紀
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、神奈川県横浜市
  • Year and Date: 2011-12-15
• [Presentation] in vitro膜タンパク質核膜局在化アッセイ系の構築 (2011)
  • Author(s): 野村菜美子、西岡優、松浦成昭、松浦脩治、檜枝美紀
  • Organizer: 第10回核ダイナミクス研究会
  • Place of Presentation: クラッセホテル北広島、北海道北広島市
  • Year and Date: 2011-10-27
• [Presentation] Nuclear envelope localized EGF receptor ligand, amphiregulin activates the cell migration via chromatin reorganization (2011)
  • Author(s): Miki Hieda, Yuhki Yokoyama, Yu Nishioka, Hisae Tanaka, Shigeki Higashiyama, Nariaki Matsuura, Shuji Matsuura
  • Organizer: EMBO Conference Series Nuclear Structure & Dynamics
  • Place of Presentation: L' sle sur la Sorgue, France
  • Year and Date: 2011-09-30
• [Presentation] Nuclear envelope localized EGF receptor ligand, amphiregulin activates the cell migration via chromatin reorganization (2011)
  • Author(s): Miki Hieda
  • Organizer: EMBL Blue Seminar
  • Place of Presentation: The European Molecular Biology Laboratory, Heidelberg, Germany
  • Year and Date: 2011-09-26
• [Presentation] 核細胞質間コミュニケーション・核からの情報発信 (2011)
  • Author(s): 檜枝美紀
  • Organizer: 第1回異分野融合ワークショップ
  • Place of Presentation: 情報通信研究機構未来ICT研究所(KARC)、兵庫県神戸市
  • Year and Date: 2011-09-08
• [Presentation] EGFファミリー増殖因子amphiregulinの核膜局在化による細胞遊走能の調節機構、(Nuclear envelope localized an EGFR ligand, amphiregulin regulates cell migration) (2011)
  • Author(s): 檜枝美紀、横山雄起、西岡優、田中ひさえ、東山繁樹、松浦成昭、松浦脩治
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学、北海道札幌市
  • Year and Date: 2011-06-27
• [Presentation] amphiregulin is translocated from the plasma membrane to the nuclear envelope and regulates cell migration (2011)
  • Author(s): Miki Hieda, An EGFR ligand
  • Organizer: 6th Asian-Pacific Organization for Cell Biology Congress(第6回アジア太平洋国際細胞生物学会)
  • Place of Presentation: Manila, Philippines
  • Year and Date: 2011-02-26
• [Presentation] A nuclear envelope localized EGFR ligand, amphiregulin, up-regulates the cancer cell migration (2011)
  • Author(s): Yuhki Yokoyama, Miki Hieda, Hisae Tanaka, Yu Nishioka, Shigeki Higashiyama, and Nariaki Matsuura
  • Organizer: International Symposium Physicochemical Field for Cenetic Activities
  • Place of Presentation: 淡路夢舞台国際会議場、兵庫県淡路市
  • Year and Date: 2011-01-26
• [Presentation] Cytoplasmic tail of EGFR ligand, amphiregulin regulates histone modification and induces the cell migration (2010)
  • Author(s): Hisae Tanaka, Miki Hieda, Yu Nishioka, Shigeki Higashiyama, Nariaki Matsuura
  • Organizer: 75th Nuclear Organization & Function, Cold Spring Harbor Symposium
  • Place of Presentation: Cold Spring Harbor Laboratory, NY, USA
  • Year and Date: 2010-06-04
• [Presentation] Intracellular domain of an EGFR ligand, amphiregulin regulates cell migration (2010)
  • Author(s): Hisae Tanaka, Miki Hieda, Yu Nishioka, Shigeki Higashiyama, Nariaki Matsuura
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場、大阪府吹田市
  • Year and Date: 2010-05-19
• [Presentation] Reverse signaling of an EGFR ligand, amphiregulin, suppresses breast cancer cell proliferation and induces the cellmigration (2010)
  • Author(s): Hisae Tanaka, Miki Hieda, Yu Nishioka, Shigeki Higashiyama, Nariaki Matsuura
  • Organizer: AACR/ JCA Joint Conference : Cancer Genomics, Epigenomics, and the Development of Novel Therapeutics
  • Place of Presentation: Waikoloa, Hawaii、USA
  • Year and Date: 2010-02-08
• [Presentation] HB-EGFの細胞膜-ER/核膜間メンブレントフィック経路の解析 (2009)
  • Author(s): Miki Hieda, Michiko Koizumi, Tomohiko Taguchi, Taro Tachibana, Nariaki Matsuura, Shigeki Higashiyama
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場、愛知県名古屋市
  • Year and Date: 2009-06-03