Molecular dissection of meiotic endocytosis

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570202
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka City University
• Principal Investigator: NAKAMURA Taro 大阪市立大学, 大学院・理学研究科, 教授 (30291082)
• Project Period (FY): 2009 – 2011
• Keywords: GFP / エンドサイトーシス / 分裂酵母 / 減数分裂 / 膜輸送

Research Abstract

The plasma membrane syntaxin 1 ortholog Psy1 is internalized and changes its localization to the forespore membrane during meiosis. The aim of this study is to address the molecular mechanism of this meiotic endocytosis of Psy1. An arrestin-like protein M

Research Products

• [Journal Article] Remarkably simple sequence requirement of the M-factor pheromone ofSchizosaccharomyces pombe (2012)
  • Author(s): Seike T, Yamagishi Y, Iio H, *Nakamura T., Shimoda C
  • Journal Title: Genetics Volume: 191 Pages: 815-822
  • Peer Reviewed
• [Journal Article] Endocytosis is essential for dynamic translocation of a syntaxin 1 ortholog during fission yeast meiosis (2011)
  • Author(s): Kashiwazaki J, Yamasaki Y, Itadani A, Teraguchi E, Maeda Y, Shimoda C, Nakamura T
  • Journal Title: Mol Biol Cell Volume: 2219 Pages: 3658-3670
  • Peer Reviewed
• [Journal Article] The fission yeast pleckstrin homology domain protein Spo7 is essential for initiation of forespore membrane assembly and spore morphogenesis. (2011)
  • Author(s): Nakamura-Kubo M, Hirata A, Shimoda C, Nakamura T
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 3442-3455
  • Peer Reviewed
• [Journal Article] Fission yeast calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane. (2010)
  • Author(s): Itadani A, Nakamura T, Hirata A, Shimoda C.
  • Journal Title: Eukaryot. Cell Volume: 9 Pages: 1925-1935
  • Peer Reviewed
• [Journal Article] Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma-membrane proteins in Schizosaccharomyces pombe (2010)
  • Author(s): Nakase M, Tani M, Morita T, Kitamoto HK, Kashiwazaki J, Nakamura T, Hosomi A, Tanaka N, Takegawa K
  • Journal Title: J. Cell Sci Volume: 123 Pages: 1578-1587
  • Peer Reviewed
• [Journal Article] Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast. (2010)
  • Author(s): Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, Bahler J, Wu JQ, Takegawa K, Tachikawa H, Pringle JR, Fukui Y
  • Journal Title: Mol. Cell Biol. Volume: 8 Pages: 2057-2074
  • Peer Reviewed
• [Journal Article] NBRP databases: databases of biological resources in Japan. (2010)
  • Author(s): Yamazaki Y, Akashi R, Banno Y, Endo T, Ezura H, Fukami-Kobayashi K, Inaba K, Isa T, Kamei K, Kasai F, Kobayashi M, Kurata N, Kusaba M, Matuzawa T, Mitani S, Nakamura Y, Nakatsuji N, Naruse K, Niki H, Nitasaka E, Obata Y, Okamoto H, Okuma M, Sato K, Serika
  • Journal Title: Nucleic Acids Res Volume: D26-30
  • Peer Reviewed
• [Journal Article] Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation (2009)
  • Author(s): Mukaiyama H, Kajiwara S, Hosomi A, Giga-Hama Y, Tanaka N, Nakamura T, Takegawa K
  • Journal Title: Microbiology Volume: 155 Pages: 3816-3826
  • Peer Reviewed
• [Journal Article] Ectopic overproduction of a meiosis-specific transcription factor induces assembly of prospore-like membranous compartments in vegetative cells of fission yeast (2009)
  • Author(s): Nakase Y, Hirata A, Shimoda C, Nakamura T
  • Journal Title: Genetics Volume: 183 Pages: 1195-1199
  • Peer Reviewed
• [Journal Article] Two fission yeast rab7 homologs, ypt7 and ypt71, play antagonistic roles in the regulation of vacuolar morphology (2009)
  • Author(s): Kashiwazaki J, Iwaki T, Takegawa K, Shimoda C, Nakamura T.
  • Journal Title: Traffic Volume: 10 Pages: 912-924
  • Peer Reviewed
• [Presentation] 分裂酵母シンタキシン1の胞子形成特異的なエンドサイトーシスの分子メカニズム (2013)
  • Author(s): 東北大学川内北キャンパス
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 20130324-27
• [Presentation] 分裂酵母シンタキシン1の胞子形成時エンドサイトーシスの分子機構 (2012)
  • Author(s): 山崎百合子、寺口絵理香、中村太郎
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] メンブレントラフィックと胞子形成 分裂酵母の胞子形成欠損株の解析から (2011)
  • Author(s): 山崎百合子、寺口絵理香、中村太郎
  • Organizer: 第44回酵母遺伝学フォーラム
  • Place of Presentation: 九州大学医学部百年講堂
  • Year and Date: 20110905-07
• [Presentation] 胞子形成特異的な分裂酵母シンタキシン1 オルソログのエンドサイトーシスの解析 (2011)
  • Author(s): 山崎百合子、寺口絵理香、中村太郎
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 20110325-27
• [Presentation] 胞子形成時のおける分裂酵母シンタキシン1オルソログの局在変化の分子メカニズム (2010)
  • Author(s): 山崎百合子、寺口絵理香、下田 親、中村太郎
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 20101207-10
• [Presentation] 減数分裂における分裂酵母シンタキシンの局在変化の分子メカニズム (2010)
  • Author(s): 寺口絵理香、柏崎 隼、下田 親、中村太郎
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 20100519-21
• [Presentation] Endocytosis during meiosis (2009)
  • Author(s): Jun Kashiwazaki, Erika Teraguchi, Akiko Itadani, Chikashi Shimoda, and Taro Nakamura
  • Organizer: International fission yeast meeting 2009
  • Place of Presentation: 東京
  • Year and Date: 20091026-31
• [Presentation] Isolation and characterization of fission yeast mutants defective in relocalization of syntaxin 1 homolog during meiosis (2009)
  • Author(s): Erika Teraguchi, Jun Kashiwazaki, Chikashi Shimoda and Taro Nakamura
  • Organizer: International fission yeast meeting 2009
  • Place of Presentation: 東京
  • Year and Date: 20091026-31
• [Presentation] Endocytosis of fission yeast syntaxin 1 orthologue during meiosis
  • Author(s): 山崎百合子、寺口絵理香、中村太郎
  • Organizer: 第63回日本細胞生物学学会
  • Place of Presentation: 北海道大学 クラーク会館