Regulation of the neural differentiation by the formation of argpyrimidine in the nucleus.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570203
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka City University
• Principal Investigator: TACHIBANA Taro 大阪市立大学, 大学院・工学研究科, 准教授 (80311752)
• Project Period (FY): 2009 – 2011
• Keywords: アルグピリミジン / 神経分化

Research Abstract

Methylglyoxal(MG) is an endogenous metabolite in glycolysis and forms stable adducts, argpyrimidine, primarily with arginine residues of intracellular proteins. The biological role of this modification in cell function is not known. In the present study, we found that a subunit of chromatin remodeling complex forms the argpyrimidine and revealed that the subunit has a critical role of neural differentiation.

Research Products

• [Journal Article] Altered expression of dermokine in skin disorders (2012)
  • Author(s): Hasegawa M, Higashi K, Yokoyama C, Yamamoto F, Tachibana T, Matsushita T, Hamaguchi Y, Saito K, Fujimoto M, Takehara K
  • Journal Title: J. Eur. Acad. Dermatol. Venereol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Chd2 interacts with H3.3 to determine myogenic cell fate (2012)
  • Author(s): Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, Kumamaru H, Saiwai H, Tsubota T, Kurumizaka H, Akashi K, Tachibana T, Imbalzano AN, Ohkawa Y
  • Journal Title: EMBO J Volume: (in press)
  • Peer Reviewed
• [Journal Article] The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach (2011)
  • Author(s): Odawara J, Harada A, Yoshimi T, Maehara K, Tachibana T, Okada S, Akashi K, Ohkawa Y
  • Journal Title: BMC Genomics Volume: 12 Pages: 516
  • Peer Reviewed
• [Journal Article] Cross talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors (2011)
  • Author(s): Mehmood, R., Yasuhara, N., Fukumoto, M., Oe, S., Tachibana, T. and Yoneda, Y
  • Journal Title: Mol. Biol. Cell Volume: 22 Pages: 3715-3724
  • Peer Reviewed
• [Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq (2011)
  • Author(s): Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y
  • Journal Title: FEBS Journal Volume: 278 Pages: 2702-2712
  • Peer Reviewed
• [Journal Article] Steroidogenic Factor 1(NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis (2011)
  • Author(s): Lai PY, Wang CY, Chen WY, Kao YH, Tsai HM, Tachibana T, Chang WC, Chung BC
  • Journal Title: Cell Death Differ Volume: 18 Pages: 1836-1844
  • Peer Reviewed
• [Journal Article] Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis (2011)
  • Author(s): Kakehashi A, Ishii N, Shibata T, Wei M, Okazaki E, Tachibana T, Fukushima S, Wanibuchi H
  • Journal Title: Toxicol Sci Volume: 119 Pages: 61-72
  • Peer Reviewed
• [Journal Article] Specific Monoclonal Antibody Against the Nuclear Pore Complex Protein, Nup96 (2010)
  • Author(s): Mizuguchi C, Oka M, Moriyama T, Tachibana T, Yoneda Y
  • Journal Title: Hybridoma Volume: 29 Pages: 551-553
  • Peer Reviewed
• [Journal Article] Live imaging system for visualizing nuclear pore complex(NPC) formation during interphase in mammalian cells (2010)
  • Author(s): Iino H, Maeshima K, Nakatomi R, Kose S, Hashikawa T, Tachibana T, Imamoto N
  • Journal Title: Genes Cells Volume: 15 Pages: 647-660
  • Peer Reviewed
• [Journal Article] The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export (2010)
  • Author(s): Oka, M., Asally, M., Yasuda, Y., Ogawa, Y., Tachibana, T. and Yoneda
  • Journal Title: Mol. Biol. Cell Volume: 21 Pages: 1885-1896
  • Peer Reviewed
• [Journal Article] Cytokeratin 8/18 as a new marker of mouse liver preneoplastic lesions (2010)
  • Author(s): Kakehashi, A., Kato, A., Inoue, M., Ishii, N., Okazaki, E., Wei, M., Tachibana, T., and Wanibuchi, H.
  • Journal Title: Toxicol. Appl. Pharmacol Volume: 242 Pages: 47-55
  • Peer Reviewed
• [Journal Article] Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family. Biochem. Biophys (2010)
  • Author(s): Jeong, Y-H., Ishikawa, K., Someya, Y., Hosoda, A., Yoshimi, T., Yokoyama, C., Kiryu-Seo, S., Kang, M-J., Tachibana, T., Kiyama, H., Fukumura, T., Kim, D-H. and Saeki, S.
  • Journal Title: Res. Commun Volume: 391 Pages: 1110-1115
  • Peer Reviewed
• [Journal Article] Generation of a Rat Monoclonal Antibody Specific for MyoD (2010)
  • Author(s): Harada, A., Ohkawa, Y., Ao, S., Odawara, J., Okada, S., Azuma, M., Nishiyama, Y., Nakamura, M., and Tachibana, T.
  • Journal Title: Hybridoma Volume: 29 Pages: 255-258
  • Peer Reviewed
• [Journal Article] Production of a Rat Monoclonal Antibody Specific for Dhx9/NDHII/RHA (2010)
  • Author(s): Kotani, M., Harada, A., Odawara, J., Azuma, M., Okada, S., Nishiyama, Y., Nakamura, M., Tachibana, T. and Ohkawa, Y
  • Journal Title: Hybridoma Volume: 29 Pages: 259-261
  • Peer Reviewed
• [Journal Article] Production and Characterization of Monoclonal Antibodies to Mouse Germ Cells (2010)
  • Author(s): Yokoyama, C., Katoh-Fukui, Y., Morohashi, K., Konno, D., Azuma, M. and Tachibana, T.
  • Journal Title: Hybridoma Volume: 29 Pages: 53-57
  • Peer Reviewed
• [Journal Article] Rat monoclonal antibody specific for the chromatin remodeling factor CHD1 (2010)
  • Author(s): Yoshimura, S., Harada, A., Odawara, J., Azuma, M., Okada, S., Nakamura, M., Ohkawa, Y. and Tachibana, T.
  • Journal Title: Hybridoma Volume: 29 Pages: 237-240
  • Peer Reviewed
• [Journal Article] Generation of a Rat Monoclonal Antibody Specific for CHD2 (2010)
  • Author(s): Harada, A., Yoshimura, S., Odawara, J., Azuma, M., Okada, S., Nakamura, M., Tachibana, T. and Ohkawa, Y.
  • Journal Title: Hybridoma Volume: 29 Pages: 173-177
  • Peer Reviewed
• [Journal Article] Rat Monoclonal Antibody Specific for Septin 9 (2010)
  • Author(s): Tachibana, T., Okazaki, E., Yoshimi, T., Azuma, M., Kakehashi A. and Wanibuchi, H.
  • Journal Title: Hybridoma Volume: 29 Pages: 169-171
  • Peer Reviewed
• [Journal Article] A Rat Monoclonal Antibody against the Chromatin Remodeling Factor, CHD5 (2010)
  • Author(s): Yoshimura, S., Yoshimi, T., Ohkawa, Y., Azuma, M. and Tachibana, T.
  • Journal Title: Hybridoma Volume: 29 Pages: 63-66
  • Peer Reviewed
• [Journal Article] Gene expression dissection of the circadian neuronal circuit of Drosophila identifies novel circadian genes (2010)
  • Author(s): Nagoshi, E., Sugino, K., Kula, E., Okazaki, E., Tachibana, T. and Nelson, S., et al.
  • Journal Title: Nature Neuroscience Volume: 13 Pages: 447-450
  • Peer Reviewed
• [Journal Article] Generation of a Rat Monoclonal Antibody Specific for Glyoxalase I (2009)
  • Author(s): Nakadate, Y., Mizuguchi, C., Azuma, M. and Tachibana, T.
  • Journal Title: Hybridoma Volume: 28 Pages: 447-450
  • Peer Reviewed
• [Journal Article] Production of a Rat Monoclonal Antibody against BRG1 (2009)
  • Author(s): Ohkawa, Y., Harada, A., Nakamura, M., Yoshimura, S. and Tachibana, T.
  • Journal Title: Hybridoma Volume: 28 Pages: 463-466
  • Peer Reviewed
• [Journal Article] Generation of Rat Monoclonal Antibodies Specific for Ad4BP/SF-1 (2009)
  • Author(s): Yokoyama, C., Komatsu, T., Ogawa, H., Morohashi, K., Azuma, M. and Tachibana, T.
  • Journal Title: Hybridoma Volume: 28 Pages: 113-119
  • Peer Reviewed
• [Journal Article] The formation of argpyrimidine, a methylglyoxal-arginine adduct, in the nucleus of neural cells (2009)
  • Author(s): Nakadate, Y., Uchida, K., Shikata, K., Yoshimura, S., Azuma, M., Hirata, T., Konishi, H., Kiyama, H. and Tachibana, T.
  • Journal Title: Biochem Biophys Res Commun Volume: 378 Pages: 209-212
  • Peer Reviewed