Dorsoventral patterning mechanism in vertebrate late development

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: SHIMADA Atsuko 東京大学, 大学院・理学系研究科, 助教 (20376552)
• Project Period (FY): 2009 – 2011
• Keywords: メダカ / Da / 背腹パターン / zic / 後期発生

Research Abstract

Vertebrates exhibit remarkable dorsoventral (DV) asymmetries in coloration and body shape. A medaka mutant Da exhibits a unique ventralized phenotype of the overall trunk morphology from late to adult stages and provides a good experimental model to investigate these late mechanisms. We show by tissue transplantation that somite-derived tissues patterned by zic1/zic4 determine the external characteristics (morphology and coloration) through long-term epithelial-mesenchymal interaction and that zic1/zic4 expression persists to the adult stage and unveils a novel dorsal domain, suggesting a continuous requirement of these genes for the maintenance of dorsal identities.

Research Products

• [Journal Article] Takeda Modular Development of the Teleost Trunk Along the Dorsoventral Axis and zic1/zic4 as selector genes in the dorsal module. (2013)
  • Author(s): Toru Kawanishi, Takuya Kaneko, Yuuta Moriyama, Masato Kinoshita, Hayato Yokoi, Tohru Suzuki, Atsuko Shimada and Hiroyuki
  • Journal Title: Development Volume: 140 Pages: 1486-1496
  • DOI: doi:10.1242/dev.088567
  • Peer Reviewed
• [Journal Article] Exoskeleton in teleosts is mesodermal in origin.Nature (2013)
  • Author(s): Atsuko Shimada, Toru Kawanishi, Takuya Kaneko, Hiroki Yoshihara, Tohru Yano, Keiji Inohaya, Masato Kinoshita, Yasuhiro Kamei, Koji Tamura & Hiroyuki Takeda Trunk
  • Journal Title: Communications
  • DOI: DOI:10.1038/ncomms2643
  • Peer Reviewed
• [Journal Article] Molecular Insights into Teleost Caudal Fin (2012)
  • Author(s): Moriyama Y, Kawanishi T, Nakamura R, Tsukahara T, Sumiyama K, Suster ML, Kawakami K, Toyoda A, Fujiyama A, Yasuoka Y, Nagao Y, Sawatari E, Shimizu A,Wakamatsu Y, Hibi M, Taira M, Okabe M, Naruse K, Hashimoto H, Shimada A, Takeda H.
  • Journal Title: Evolution. Curr.Biol. Volume: 22 Pages: 601-617
  • DOI: DOI 10.1016/j.cub.2012.01.063
  • Peer Reviewed
• [Journal Article] The art of medaka genetics and genomics: what makesthem so unique? (2012)
  • Author(s): Takeda, H., Shimada, A.
  • Journal Title: Annual Review of Genetics Volume: 44 Pages: 217-41
  • Peer Reviewed
• [Presentation] Mesodermal origin of scales and fins of fish- Insight into origin and evolution of mineralized skeleton in vertebrates (2011)
  • Author(s): Shimada, A., Kawanishi, T., Kaneko, T., Yoshihara, H., Yano, T., Inohaya, K., Kamei, Y., Kinoshita, M., Tamura, K., Takeda, H.
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 宜野湾
  • Year and Date: 20110518-21
• [Presentation] zic1 and zic4 in late embryogenesis mediate patterning for adult dorsoventral trunk (2011)
  • Author(s): Kawanishi, T. Moriyama, Y., Shimada, A.,
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 宜野湾
  • Year and Date: 20110518-21
• [Presentation] 発生後期における zic1/zic4 を介した背腹パターン決定機構 (2011)
  • Author(s): 島田敦子、河西通、守山裕大、武田洋幸
  • Organizer: 第84回日本生化学会大会(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] メダカ変異体Daを用いた体幹部背腹パターン形成メカニズムの解析 (2010)
  • Author(s): 河西通
  • Organizer: BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] メダカ外骨格(鱗、正中鰭)の骨形成細胞の細胞系譜解析 (2010)
  • Author(s): 島田敦子、猪早敬二、伊藤麻紀子、河西通、武田洋幸
  • Organizer: 日本動物学会第81回年会
  • Place of Presentation: 目黒
  • Year and Date: 20100923-25
• [Presentation] zic 遺伝子の発現解析で明らかとなったメダカ体幹部の新しい背側アイデンティティ (2010)
  • Author(s): 兼子拓也、河西通、守山裕大、木下政人、田中実、塚原達也、島田敦子、越田澄人、武田洋幸
  • Organizer: 日本動物学会第81回年会
  • Place of Presentation: 目黒
  • Year and Date: 20100923-25
• [Presentation] Embryonic origin of osteoblastsin scales and fins of medaka fish (2010)
  • Author(s): Shimada, A., Kawanishi, T., Inohaya, K.,Takeda, H.
  • Organizer: 2010SDB-JSDB Joint Meeting, Albuquerque
  • Place of Presentation: U.S.A.
  • Year and Date: 20100805-09
• [Presentation] zic1 and zic4 expression in the somite regulates dorsalization of the fish trunk structures (2010)
  • Author(s): Kawanishi, T., Moriyama, Y. , Nakamura, R., Shimada, A., Takeda, H.
  • Organizer: 2010 SDB-JSDB Joint Meeting, Albuquerque
  • Place of Presentation: U.S.A.
  • Year and Date: 20100805-09
• [Presentation] The zic genes as master regulators in dorsoventral patterning of the fish trunk structures (2010)
  • Author(s): Moriyama, Y., Nakamura, R., Kawanishi, T., Shimada, A., Takeda, H.
  • Organizer: 2nd Joint Meeting of the SFBD and JSDB
  • Place of Presentation: Paris, France
  • Year and Date: 20100526-28
• [Presentation] Analysis of mechanisms underlying the development of the external morphology using the medaka mutant, Double anal fin (Da) - Analysis of the somite-specific enhancer of the zic genes in medaka: toward understanding of the evolution of the the external morphology in vertebrates (2009)
  • Author(s): Moriyama, Y., Shimada, A., Naruse, K., Fujiyama, A., Toyoda, A., Takeda, H.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] メダカDa変異体を用いた体節移植から明らかになった体幹部背腹パターン形 (2009)
  • Author(s): 河西通、守山裕大、島田敦子、武田洋幸
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 20090917-20
• [Presentation] メダカ鱗および鰭条における骨形成細胞の細胞系譜解析 (2009)
  • Author(s): 島田敦子、河西通、武田洋幸
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 20090917-20
• [Presentation] The loss of zic1/zic4 expression in the somite is responsible for the ventralizing phenotype in the medaka mutant Da (2009)
  • Author(s): Kawanishi, T., Shimada, A., Takeda, H.
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 新潟
  • Year and Date: 20090528-31
• [Presentation] Analysis of the somite-specific enhancer of the zic genes in medaka: toward understanding of the evolution of the the external morphology in vertebrates (2009)
  • Author(s): Moriyama,Y., Shimada, A., Naruse, K., Fujiyama, A., Toyoda, A., Takeda, H.
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 新潟
  • Year and Date: 20090528-31
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hassei/