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2011 Fiscal Year Final Research Report

Analysis of the transition mechanism from mitosis to meiosis on spermatogenic cells by using cybrid model.

Research Project

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Project/Area Number 21570228
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionYamaguchi University (2010-2011)
Nagasaki University (2009)

Principal Investigator

SATO Yoko  山口大学, 農学部, 学術研究員 (50398963)

Co-Investigator(Kenkyū-buntansha) KOJI Takehiko  長崎大学, 大学院・医歯薬学総合研究科, 教授 (30170179)
Project Period (FY) 2009 – 2011
Keywords精子形成 / 減数分裂 / 細胞培養系 / 細胞融合
Research Abstract

Meiosis is the special option for germ line cells to determine the cell fate. The aim of this study is to know the factors, which are involved in the induction or inhibition of meiosis in the spermatogonia. In the beginning, we tried to establish the experimental condition to make hetero karyotic cell by electronic fusion technique using both the competent spermatogenic cells, which can show meiotic progression, and the non-competent spermatogenic cells. However, the competent spermatogenic cells could not induce meiosis in the non-competent spermatogenic cells under hetero karyotic cell condition. To remove the effects of the donor nuclei from the recipient cells, cytoplasts(enucleated cytoplasm) from the competent spermatogenic cells were electro-fused with non-competent spermatogenic cells. The nuclei of the produced cybrids(cytoplasmic hybrid) were analyzed morphologically. These cybrids showed morphological changes in the nuclei similar to zygotene spermatocytes immediately after fusion. These results suggested that the cytoplasm of the competent spermatogenic cells contain the factor, which can induce meiotic progression in the non-competent spermatogenic cells. Further analysis including the expression of the specific molecular marker of the meiosis will need to be taken.

  • Research Products

    (17 results)

All 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (6 results)

  • [Journal Article] Long-term preservation of chilled canine semen using vitamine C in combination with green tea polyphenol2012

    • Author(s)
      M. Wittayarat, K. Taichi, R. Kodama, Z. Namula, K. Chatdarong, M. Techakumphu, Y. Sato, M. Tachiguchi, T. Otoi
    • Journal Title

      Cryo Letters

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Effect of sericin on preimplantation development of bovine embryos cultured individually2012

    • Author(s)
      T. Isobe, Y. Ikebata, T. Onitsuka, M. Wittayarat, Y. Sato, M. Taniguchi and T. Otoi.
    • Journal Title

      Theiogenology

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Effects of(-)-epigallocatechin gallate on the motility and penetrability of frozen-thawed boar spermatozoa incubated in the fertilization medium2012

    • Author(s)
      Y. Kaedei, M. Naito, H. Naoi, Y. Sato, M. Taniguchi, F. Tanihara, K. Kikuchi, T. Nagai, and T. Otoi
    • Journal Title

      Reprod. Dom. Anim

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Glycoconjugates recognized by peanut agglutinin lectin in the inner acellular layer of the lamina propria of seminiferous tubules in human testes showing impaired spermatogenesis.2012

    • Author(s)
      Y. Sato, S. Nozawa, M. Yoshiike, T. Otoi and T. Iwamoto
    • Journal Title

      Hum. Reprod

      Volume: 27 Pages: 659-668

    • Peer Reviewed
  • [Journal Article] Gene suppression of mouse testis in vivo using small interfering RNA derived from plasmid vectors.2012

    • Author(s)
      T. Takizawa, T. Ishikawa, T. Kosuge, Y. Mizuguchi, Y. Sato, T. Koji, Y. Araki and T. Takizawa
    • Journal Title

      Acta Histochem. Cytochem

      Volume: 45 Pages: 77-81

    • Peer Reviewed
  • [Journal Article] Estrogen-dependent regulation of sodium/ hydrogen exchanger-3(NHE3) expression via estrogen receptor B in proximal colon of pregnant mice2012

    • Author(s)
      N. Choijookhuu, Y. Sato, T. Nishino, D. Endo, Y. Hishikawa and T. Koji
    • Journal Title

      Histochem. Cell Biol

      Volume: 137 Pages: 575-587

    • Peer Reviewed
  • [Journal Article] Follicle formation in the canine ovary after autografting to a peripheral site2012

    • Author(s)
      T. Terazono, Y. Kaedei, F. Tanihara, Z. Namura, V. L. Viet, M. Takagi, M. Inoue, Y. Sato, M. Taniguchi and T. Otoi
    • Journal Title

      Reprod. Domest. Anim

      Volume: 47 Pages: 16-21

    • Peer Reviewed
  • [Journal Article] Development and subsequent cryotolerance of domestic cat embryos cultured in serum-free and serum-containing media2011

    • Author(s)
      M. Murakami, Y. J. Dong, T. Suzuki, M. Taniguchi, Y. Kaedei, Y. Sato, F. Tanihara and T. Otoi
    • Journal Title

      Cryobiology

      Volume: 63 Pages: 170-174

    • Peer Reviewed
  • [Journal Article] Effects of cryoprotectant agents and equilibration methods on developmental competence of porcine oocytes2011

    • Author(s)
      M. Taniguchi, R. Arikawa, Y. Kaedei, F. Tanihara, Z. Namula, V. L. Viet, Y. Sato and T. Otoi
    • Journal Title

      Cryo Letters

      Volume: 32 Pages: 410-414

    • Peer Reviewed
  • [Journal Article] Xenografting of testicular tissue from an infant human donor results in accelerated testicular maturation2010

    • Author(s)
      Y. Sato, S. Nozawa, M. Yoshiike, M. Arai, C. Sasaki and T. Iwamoto
    • Journal Title

      Human Reprod

      Volume: 25 Pages: 1113-1122

    • Peer Reviewed
  • [Journal Article] Studying spermatogenesis by using in vivo and in vitro models : Advantages and disadvantages of these models for practical use

    • Author(s)
      Y. Sato, M. Taniguchi, T. Otoi
    • Journal Title

      J. Vet. Sci. Tech

      Volume: (in press)

    • Peer Reviewed
  • [Presentation] Application of in vitro spermatogenesis model2011

    • Author(s)
      Y. Sato
    • Organizer
      JICA program for Southeast Asia
    • Place of Presentation
      Yamaguchi University, Yamaguchi
    • Year and Date
      2011-10-11
  • [Presentation] 造精機能障害を示す精巣について2011

    • Author(s)
      佐藤陽子
    • Organizer
      脂質栄養と性差に関するオープンリサーチセンター整備事業講演会
    • Place of Presentation
      金城学院大学,名古屋
    • Year and Date
      2011-08-19
  • [Presentation] Effect of 5-aza-2'-deoxycytidine(5-aza-dC) on epigenetic modifications in spermatogenic cells of mouse2010

    • Author(s)
      N. Song, T. Nishino, Y. Sato, Y. Hishikawa, T. Koji
    • Organizer
      第51回日本組織細胞化学会総会
    • Place of Presentation
      秋葉原コンベンションセンター,東京
    • Year and Date
      2010-09-04
  • [Presentation] Differentiation stage-specific changes of DNA methylation and histone H3 modifications in mouse spermatogenesis2009

    • Author(s)
      N. Song, Y. Hishitaka, Y. Sato, T. Koji
    • Organizer
      第50回日本組織細胞化学会総会
    • Place of Presentation
      ピアザ淡海,大津
    • Year and Date
      2009-09-26
  • [Presentation] 生殖細胞に発現しているTex101に関するin vivoノックダウン解析の試み2009

    • Author(s)
      瀧澤敬美・石川朋子・石橋宰・後藤忠・佐藤陽子・小路武彦・荒木慶彦・瀧澤俊広
    • Organizer
      第50回日本組織細胞化学会総会
    • Place of Presentation
      ピアザ淡海,大津
    • Year and Date
      2009-09-26
  • [Presentation] マウス生殖細胞アポトーシス誘導におけるミトコンドリア動態とERβ発現の関与2009

    • Author(s)
      菱川善隆・安樹才・荒木慶彦・松田賢一・河田光博・小路武彦
    • Organizer
      第114回日本解剖学会総会
    • Place of Presentation
      岡山理科大学,岡山
    • Year and Date
      2009-03-29

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Published: 2013-07-31  

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