2011 Fiscal Year Final Research Report
Molecular mechanisms of circadian regulation in CAM
| Project/Area Number |
21580016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Crop science/Weed science
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| Research Institution | Kagawa University (2010-2011)
Saga University (2009) |
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Principal Investigator |
AGARIE Sakae 香川大学, 農学部, 准教授 (50304879)
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| Project Period (FY) |
2009 – 2011
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| Keywords | CAM植物 / 概日リズム / 光合成 / 生物時計 |
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| Research Abstract |
To elucidate molecular the mechanisms for circadian regulation of CAM, the 5' flanking region of CAM-related genes(ppcD, Ppck, Ppc1, McPpck1, Mod1) were isolated from CAM plants such as Mesembryanthemum crystallinum, Kalanchoe pinnata, K. fedtschenkoi. In the regions of these plants, cis-regulatory elements responsible for ABA, ethylene, drought and salt stresses and circadian regulate and the binding sites of Myb and Dof were found. Transient-expression assays indicated that the sequence 1600 to-1100 of McPpck of M. crystallinum was sufficient to drive the expression of the reporter gene. We applied Agrobacterium-mediated in planta transformation procedure to M. crystallinum. Transformation rates using cotyledons, side shoots and seeds were 20%, 90% and 70%, respectively.
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