Studies of microbial community structure and function for pathogen bank in field margins

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Chiba University
• Principal Investigator: SHISHIDO Masahiro 千葉大学, 大学院・園芸学研究科, 准教授 (80302537)
• Project Period (FY): 2009 – 2011
• Keywords: 感染・増殖 / 病原菌生態 / 微生物群集 / フィールド・マージン / 病原菌バンク / リアルタイムPCR / 土壌伝染性病害

Research Abstract

Ecology of black root rot of cucurbittaceae and white root rot of Japanese pear were studied to elucidate functions for pathogen bank in field margins. After developing real-time PCR protocols to quantify these plant pathogens, relationships between pathogen quantities and microbial community structures of both in-fields and field margins. The research results suggested that pathogens were least proliferate in field margins, mostly remaining inside the fields, and affected only particular groups of microorganisms.

Research Products

• [Journal Article] Development of real-time PCR assayusing Taq Man probe for specific and quantitative detection of Rosellinia necatrix in plant and soil (2012)
  • Author(s): Shishido, M., Kubota, I., Nakamura, H
  • Journal Title: Journal of General Plant Pathology Volume: 78(2) Pages: 115-120
  • DOI: DOI:10.1007/s10327-012-0366-x
• [Journal Article] Effect of jasmonates on ethylene biosynthesis and aroma volatile emission in Japanese apricot infected by a pathogen(Colletotrichum gloeosporioides) (2011)
  • Author(s): Nimitkeatkai, H., Shishido, M., Okawa, K., Ohara, H., Ban, Y., Kita, M., Moriguchi, T., Ikeura, H., Hayata, Y., Kondo, S
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 59(12) Pages: 6423-6429
  • DOI: DOI:10.1021/jf2010996
• [Journal Article] Plant disease management in protected horticulture(review) (2011)
  • Author(s): Shishido M
  • Journal Title: Hort Research Volume: 65 Pages: 7-18
  • URL: http://www.h.chiba-u.ac.jp/chiiki/hortresearch.html
• [Journal Article] PCR-based assays to detect and quantify Phomopsis sclerotioides in plants and soil (2010)
  • Author(s): Shishido, M., Sato, K., Yoshida, N., Tsukui, R., Usami, T
  • Journal Title: Journal of General Plant Pathology Volume: 76(1) Pages: 21-30
  • DOI: DOI:10.1007/s10327-009-0209-6
• [Presentation] Development of real-time PCR assay using Taq Man probefor detection and quantification of Monosporascus cannonballus in plant andsoil (2012)
  • Author(s): Shishido, M., Yoshida, N., Tsukui1, R., Yokoyama, T., Usami, T
  • Organizer: The 2nd Korea-Japan Joint Symposium,The Phytopathological Society of Japan(PSJ) and The Korean Society of Plant Pathology (KSPP)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120327-30
• [Presentation] TaqMan probeを用いたリアルタイムPCRによる白紋羽病菌の定量と検出 (2011)
  • Author(s): 宍戸雅宏, 久保田斎, 中村仁
  • Organizer: 日本土壌微生物学会
  • Place of Presentation: 大崎市鳴子公民館
  • Year and Date: 20111125-26
• [Presentation] TaqManプローブを用いたリアルタイムPCRによるPhomopsis sclerotioides検出および定量法の改良 (2011)
  • Author(s): 宍戸雅宏, 久保田斎, 大橋竜也
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 20110327-29
• [Presentation] ホモプシス根腐病菌はウリ科作物に宿主特異性を示さない (2010)
  • Author(s): 宍戸雅宏
  • Organizer: 平成22年度日本植物病理学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20100418-20