Development of novel drug screening process by using Drosophila disease models and silk worm Cypovirus function

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: YAMAGUCHI Masamitsu 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
• Co-Investigator(Kenkyū-buntansha): MORI Hajime 京都工芸繊維大学, 工芸科学研究科, 教授 (80201812) ; HASHIMOTO Reina 京都工芸繊維大学, ベンチャーラボラトリー, 研究員 (20467492)
• Project Period (FY): 2009 – 2011
• Keywords: 昆虫 / 遺伝子 / 応用動物 / 生体分子 / 遺伝学

Research Abstract

β-sarcoglycan contains EGF-like amino acid sequences in its extracellular domain. In vitro analyses demonstrated thatβ-sarcoglycan can interacts with EGFR via this extracellular domain. Double mutant for Syntrophin1and2has been established. Analyses with this double mutant clarified in vivo roles of these two genes. Recombinantβ-sarcoglycan tagged with Cypovirus VP3has been successfully expressed in cultured Drosophila cells.

Research Products

• [Journal Article] Fibroblast growth factor-2(Fgf2) is an important factor that maintains cellular immaturity and contributes to aggressiveness of osteosarcoma (2012)
  • Author(s): Shimizu, T., Ishikawa, T., Iwai, S., Ueki, A., Sugihara, E., Onishi, N., Kuninaka, S., Miyamoto, T., Toyama, Y., Ijiri, H., Mori, H., Matsuzaki, Y., Yaguchi, T., Nishio, H., Kawakami, Y., Ikeda, Y., Saya, H.
  • Journal Title: Mol. Cancer Res Volume: 10 Pages: 454-468
  • Peer Reviewed
• [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression (2012)
  • Author(s): Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A. Vazquez, M., Neumann, A., Yamaguchi, M. and Zurita, M.
  • Journal Title: Nucleic Acids Res Volume: 40(4) Pages: 1460-1474
  • Peer Reviewed
• [Journal Article] The warts gene as a novel target of the Drosophila DRE/DREF transcription pathway (2012)
  • Author(s): Fujiwara, S., Ida, H., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
  • Journal Title: Am J. Cancer Res Volume: 2(1) Pages: 36-44
  • Peer Reviewed
• [Journal Article] Transcription factor NF-Y is involved in differentiation of R7 photoreceptor cell in Drosophila (2012)
  • Author(s): Yoshioka, Y., Ly, L. L. and Yamaguchi, M.
  • Journal Title: Biology Open Volume: 1 Pages: 19-29
  • DOI: doi:10.1242/bio.2011013
  • Peer Reviewed
• [Journal Article] The use of leukemia inhibitory factor immobilized on virus-derived polyhedra to support the proliferation of mouse embryonic and induced pluripotent stem cells (2011)
  • Author(s): Nishishita, N., Ijiri, H., Takenaka, C., Kobayashi, K., Goto, K., Kotani, E., Itoh, T., Mori, H., Kawamata, S.
  • Journal Title: Biomaterials Volume: 32 Pages: 3555-3563
  • Peer Reviewed
• [Journal Article] Characterization of a novel C-type lectin cDNA, CLEM20cDNA, specifically expressed in mouthparts of the flesh fly Sarcopahaga peregrina (2011)
  • Author(s): Yamamoto-Kihara, M., Kotani, E., Mori, H.
  • Journal Title: J. Insect Biotech. Sericol Volume: 80 Pages: 31-39
  • Peer Reviewed
• [Journal Article] Regulation of intestinal stem cell proliferation by human methyl-CpG-binding protein-2in Drosophila (2011)
  • Author(s): Lee, S.-H., Kim, I-J., Kim, J.-G., Kim, Y.-S., Yamaguchi, M. and Yoo, M.-A.
  • Journal Title: Cell Struct. Funct Volume: 36(2) Pages: 197-208
  • Peer Reviewed
• [Journal Article] Essential role of Duox in the stabilization of Drosophila wing (2011)
  • Author(s): Tu Anh, N. T., Nishitani, M., Harada, S., Yamaguchi, M. and Kamei, K.
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 33244-33251
  • Peer Reviewed
• [Journal Article] Identification of nuclear localization signals of Drosophila G9a histone H3 methyltransferase (2011)
  • Author(s): Kato, Y., Ushijima, Y. and Yamaguchi, M.
  • Journal Title: Cell Struct. Funct Volume: 36 Pages: 121-129
  • Peer Reviewed
• [Journal Article] DREF is critical for Drosophila bristle development by regulating endoreplication in shaft cells (2011)
  • Author(s): Kawamori, A. and Yamaguchi, M.
  • Journal Title: Cell Struct. Funct Volume: 36 Pages: 103-119
  • Peer Reviewed
• [Journal Article] A Drosophila model for the screening of bioavailable NADPH oxidase inhibitors and antioxidants (2011)
  • Author(s): Tu Anh, N. T., Nishitani, M., Harada, S., Yamaguchi, M. and Kamei, K.
  • Journal Title: Mol. Cell. Biochem Volume: 352 Pages: 91-98
  • Peer Reviewed
• [Journal Article] NF-Y transcription ally regulates the Drosophila p53 gene (2011)
  • Author(s): Tue, N. T., Yoshioka, Y. and Yamaguchi, M.
  • Journal Title: Gene Volume: 473 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Immobilization of protein kinase C into cypovirus polyhedra (2010)
  • Author(s): Ijiri, H., Nakatani, T., Ido, H., Hamada, N., Kotani, E., Mori, H.
  • Journal Title: J. Insect Biotech. Sericol Volume: 79 Pages: 15-20
  • Peer Reviewed
• [Journal Article] Rapid and accurate method for isolation of recombinant baculovirus with an expanded host range (2010)
  • Author(s): Watanabe S, Ohta M, Kokuho T, Mori H, Inumaru S.
  • Journal Title: J. Biosci. Bioeng Volume: 110 Pages: 66-68
  • Peer Reviewed
• [Journal Article] Secreted frizzled related protein4reduces fibrosis scar size and ameliorates cardiac function after ischemic injury (2010)
  • Author(s): Matsushima, K., Suyama, T., Takenaka, C., Nishishita, N., Ikeda, K., Ikada, Y., Sawa, Y., Jakt, L.-M., Mori, H., Kawamata, S
  • Journal Title: Tissue Engineering Part A Volume: 16 Pages: 3329-3341
  • Peer Reviewed
• [Journal Article] Drosophila Syntrophins are involved in locomotion and regulation of synaptic morphology (2010)
  • Author(s): Nagai, R., Hashimoto, R. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res Volume: 316 Pages: 2313-2321
  • Peer Reviewed
• [Journal Article] Regulation of the Drosophila p38b gene by the transcription factor DREF in adult midgut (2010)
  • Author(s): Park, J.-S., Kim, Y.-S., Kim, J.-G., Lee, S.-H., Park, S.-Y., Yamaguchi, M. and Yoo, M.-A.
  • Journal Title: Biochim. Biophys. Acta Volume: 1799 Pages: 510-519
  • Peer Reviewed
• [Journal Article] Role of DREF in transcriptional regulation of the Drosophila p53gene (2010)
  • Author(s): Tue, N. T., Thao, D. T. P. and Yamaguchi, M.
  • Journal Title: Oncogene Volume: 29 Pages: 2060-2069
  • Peer Reviewed
• [Journal Article] Syntrophin-2is required for eye development in Drosophila (2010)
  • Author(s): Nagai, R., Hashimoto, R., Tanaka, Y., Taguchi, O., Sato, M., Matsukage, A. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res Volume: 316 Pages: 272-285
  • Peer Reviewed
• [Journal Article] The atomic structure of baculovirus polyhedra reveals the independent emergence of infectious crystals in DNA and RNA viruses (2009)
  • Author(s): Coulibaly, F., Chiu, E., Gutmann, S., Rajendran, C., Haebel, P. W., Ikeda, K., Mori, H., Ward, V. K., Schulze-Briese, C., Metcalf, P
  • Journal Title: Proc. Nat. Acad. Sci Volume: 106 Pages: 22205-22210
  • Peer Reviewed
• [Journal Article] Structure-based targeting of bioactive proteins into cypovirus polyhedra and application to immobilized-cytokines for mammalian cell culture (2009)
  • Author(s): Ijiri, H., Coulibaly, F., Nishimura, G., Nakai, D., Chiu, E., Takenaka, C., Ikeda, K., Nakazawa, H., Hamada, N., Kotani, E., Metcalf, P., Kawamata, S., Mori, H
  • Journal Title: Biomaterials Volume: 30 Pages: 4297-4308
  • Peer Reviewed
• [Journal Article] Molecular Characterization of a TIA-1 like RNA-binding protein in the cells derived from the fall armyworm (2009)
  • Author(s): Muto, S., Tanabe, T., Matsumoto, E., Mori, H., Kotani, E
  • Journal Title: Spodoptera frugiperda(Lepidoptera : Noctuidae). Biosci. Biotechnol. Biochem Volume: 73 Pages: 648-656
  • Peer Reviewed
• [Journal Article] Functional analysis of the gene of BmTRN-1, an RNA-binding protein homologous to mammalian TIA-1from the silkworm (2009)
  • Author(s): Muto, S., Matsumoto, E., Tanabe, T., Mori, H., Kotani, E.
  • Journal Title: Bombyx mori ; a study of it's over expression and sub-cellular distribution during the baculovirus infection process. J. Insect Biotech. Sericol Volume: 78 Pages: 39-51
  • Peer Reviewed
• [Journal Article] Pret porter, a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells (2009)
  • Author(s): Kuraishi, T., Nakagawa, Y., Nagaosa, K., Hashimoto, Y., Ishimoto, T., Moki, T., Fujita, Y., Nakayama, H., Dohmae, N., Shiratsuchi, A., Yamamoto, N., Ueda, K., Yamaguchi, M., Awasaki, T. and Nakanishi, Y.
  • Journal Title: EMBO J Volume: 28 Pages: 3868-3878
  • Peer Reviewed
• [Journal Article] A novel Drosophila Girdin-like protein is involved in Akt pathway control of cell size (2009)
  • Author(s): Puseenam A., Yoshioka Y., Nagai, R., Hashimoto R., Suyari, O., Itoh, M., Enomoto, A., Takahashi, M. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res Volume: 315 Pages: 3370-3380
  • Peer Reviewed
• [Journal Article] Identification of the Drosophila Mes4 gene as a novel target of the transcription factor DREF (2009)
  • Author(s): Suyari, O., Ida, H., Yoshioka, Y., Kato, Y., Hashimoto, R. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res Volume: 315 Pages: 1403-1414
  • Peer Reviewed
• [Journal Article] Genetic screening for modifiers of the DREF pathway in Drosophila melanogaster : Identification and characterization of HP6 as a novel target of DREF (2009)
  • Author(s): Ida, H., Suzusho, N., Suyari, O., Yoshida, H., Ohno, K., Hirose, F., Itoh, M. and Yamaguchi, M.
  • Journal Title: Nucleic Acids Res Volume: 37 Pages: 1423-1437
  • Peer Reviewed
• [Presentation] キイロショウジョウバエDOCK family CG31048遺伝子のR7光受容細胞分化への関与 (2011)
  • Author(s): 江口晃一、吉岡泰秀、吉田英樹、宮田清司、日合弘、山口政光
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] Involvement of dMLF in JNK signaling pathway (2011)
  • Author(s): Yanai, H., Nakao, Y., Yoshioka, Y., Yoshida, H. and Yamaguchi, M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] ショウジョウバエ骨髄性白血病因子dMLFのJNKシグナル伝達経路への関与 (2010)
  • Author(s): 梁井啓史、中尾祐美子、吉岡泰秀、吉田英樹、山口政光
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸市・神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 遺伝性神経変性疾患ALS(筋萎縮性側索硬化症Amyotrophic Lateral Sclerosis)モデルショウジョウバエを用いた病態解析 (2010)
  • Author(s): 笹山博司、島村真依、徳田隆彦、山口政光、中川正法
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸市・神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Genetic screen for Drosophila myeloid leukemia factor(dMLF) interactants and analysis of dMLF in JNK pathway (2009)
  • Author(s): Yanai, H., Nakao, Y., Yoshioka, Y. and Yamaguchi, M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] DREF, a novel transcriptional regulatory factor of the Drosophila p53gene (2009)
  • Author(s): Tue, N. T., Thao, D. T. P. and Yamaguchi, M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] In vivo function of Drosophila Syntrophin, a component of the DGC (2009)
  • Author(s): Nagai, R., Hashimoto, R., Tanaka, Y., Sato, M., Matsukage, A. and Yamaguchi, M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] Cypovirus polyhedra : structure and application (2009)
  • Author(s): Mori, H.
  • Organizer: International Symposium on Recent Bio-Research Works in KIT of Japan and PNU of Korea
  • Place of Presentation: 韓国、釜山市・釜山大学
  • Year and Date: 2009-09-11
• [Remarks] 京都工芸繊維大学・応用生物学部門・染色体工学研究室ホームページ
  • URL: http://www.bio.kit.ac.jp/chromosome/PUBLICATION.html