Quantification of insulin-like growth factor binding proteins in salmon

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580214
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: SHIMIZU Munetaka 北海道大学, 大学院・水産科学研究院, 講師 (90431337)
• Project Period (FY): 2009 – 2011
• Keywords: 成長 / ホルモン / サケ科魚類 / 免疫測定系 / 発現解析

Research Abstract

The present study established time-resolved fluoroimmunoassay and real-time quantitative PCR for salmon insulin-like growth factor (IGF)-I and an IGF-binding protein (IGFBP-1b). Feeding and fasting experiment using individually-tagged masu salmon revealed that IGF-I and IGFBP-1b generally show positive and negative correlations with growth rate, respectively. The present study suggests that these components are useful as growth indices in salmon.

Research Products

• [Journal Article] Effects of short-term starvation on ghrelin, GH-IGF system, and IGF-binding proteins in Atlantic salmon (2011)
  • Author(s): Hevroy, E. M., Azpeleta, C., Shimizu, M., Lanzen, A., Kaiya, H., Espe, M, and Olsvik, P. A.
  • Journal Title: Fish Physiology and Biochemistry Volume: 37 Pages: 217-232
  • DOI: DOI:10.1007/s10695-010-9434-3
  • Peer Reviewed
• [Journal Article] Circulating salmon 41-kDa insulin-like growth factor binding protein (IGFBP) is not IGFBP-3 but an IGFBP-2 subtype (2011)
  • Author(s): Shimizu, M., Suzuki, S., Horikoshi, M., Hara, A., and Dickhoff, W. W.
  • Journal Title: General and Comparative Endocrinology Volume: 171 Pages: 326-331
  • DOI: DOI:10.1016/j.ygcen.2011.02.013
  • Peer Reviewed
• [Journal Article] Circulating salmon 28-and 22-kDa insulin-like growth factor binding proteins (IGFBPs) are co-orthologs of IGFBP-1 (2011)
  • Author(s): Shimizu, M., Kishimoto, K., Yamaguchi, T., Nakano, Y., Hara, A., and Dickhoff, W. W.
  • Journal Title: General and Comparative Endocrinology Volume: 174 Pages: 97-106
  • DOI: DOI:10.1016/j.ygcen.2011.08.005
  • Peer Reviewed
• [Journal Article] Postprandial changes in plasma growth hormone, insulin, insulin-like growth factor (IGF)-I and IGF-binding proteins in coho salmon fasted for varying periods (2009)
  • Author(s): Shimizu, M., Cooper, K. A., Dickhoff, W. W., and Beckman, B. R.
  • Journal Title: American Journal of Physiology-Regulatory, Integrative, and Comparative Physiology Volume: 297 Pages: R352-R361
  • DOI: DOI:10.1152/ajpregu.90939.2008
  • Peer Reviewed
• [Journal Article] Normalizing for biology : accounting for technical and biological variation in levels of reference gene and insulin-like growth factor 1 (igf1) transcripts in fish livers
  • Author(s): Metzger, D. C., Luckenbach, J. A., Shimizu, M., and Beckman, B. R.
  • Journal Title: Comparative Biochemistry and Physiology Volume: (in press)
  • DOI: DOI:10.1016/j.cbpa.2012.04.014
  • Peer Reviewed
• [Presentation] サクラマスの銀化に伴うインスリン様成長因子-Iの変化と鰓Na+, K+-ATPaseとの関係 (2012)
  • Author(s): 下村考弘・中嶋拓郎・堀越萌李・飯嶋亜内・卜部浩一・水野伸也・平松尚志・原彰彦・清水宗敬
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(品川、東京)
  • Year and Date: 2012-03-28
• [Presentation] 生理学を武器にして動物生態学をおもしろくできないだろうか? (2011)
  • Author(s): 清水宗敬
  • Organizer: 日本生態学会第58回全国大会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
  • Year and Date: 20110308-12
• [Presentation] サクラマスにおけるインスリン様成長因子結合蛋白-1の発現パターンと成長との関係 (2011)
  • Author(s): 下村孝弘・中嶋拓郎・堀越萌李・飯嶋亜内・卜部浩一・水野伸也・平松尚志・原彰彦・清水宗敬
  • Organizer: 第5回サケ学研究会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-12-12
• [Presentation] 1回の給餌に対するサケ科魚類の血中インスリン様成長因子-Iと結合蛋白の反応 (2010)
  • Author(s): 清水宗敬・Kathleen A. Cooper・Walton W. Dickhoff・Brian R. Beckman
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 20100922-25
• [Presentation] サクラマスにおける海水適応能の発達とインスリン様成長因子-Iの発現パターン (2010)
  • Author(s): 下村考弘・堀越萌李・中嶋拓郎・飯嶋亜内・卜部浩一・水野伸也・平松尚志・原彰彦・清水宗敬
  • Organizer: 第4回サケ学研究会
  • Place of Presentation: 北海道大学(函館市)
  • Year and Date: 2010-12-18
• [Presentation] Which IGF-binding proteins should we look at during smoltification? (2009)
  • Author(s): Shimizu, M., Suzuki, S., Horikoshi, M., Hara, A., and Dickhoff, W. W.
  • Organizer: 8th International Workshop on Smoltification
  • Place of Presentation: Oregon State University (Corvallis, Oregon, USA)
  • Year and Date: 20090920-24
• [Presentation] サケ科魚類は成長のための「定期預金」を持っていないか? (2009)
  • Author(s): 清水宗敬・鈴木せいら・堀越萌李・原彰彦・Walton W. Dickhoff
  • Organizer: 第3回サケ学研究会
  • Place of Presentation: 北海道立水産孵化場(恵庭市)
  • Year and Date: 2009-12-05
• [Remarks]
  • URL: http://www.geocities.jp/fishvitellogenin/ms/Welcome.html