Investigation of Fish hematopoiesis

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580236
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nihon University
• Principal Investigator: MORITOMO Tadaaki 日本大学, 生物資源科学部, 准教授 (20239677)
• Project Period (FY): 2009 – 2011
• Keywords: 魚病

Research Abstract

Investigation of Fish hematopoiesis
Co-cultivation of carp hematopoietic cells with supporting cell layer resulted in selective proliferation of CD4 single positive T cells. Using this culture system, several helper T cell-like clones were established. Carp hematopoietic cells were also cultured in semi-solid media in the presence of recombinant erythropoietin, thrombopoietin and stem cell factor. Cells colonies derived from progenitors of erythrocyte and thrombocytes were formed.

Research Products

• [Journal Article] Hematopoietic cell populations in dolphin bone marrow : Analysis of colony formation and differentiation (2011)
  • Author(s): Segawa, T., Itou, T., Suzuki, M., Moritomo, T., Nakanishi, T., and Sakai, T
  • Journal Title: Results in Immunology Volume: 1 Pages: 1-5
  • Peer Reviewed
• [Journal Article] クローンギンブナの再生医学研究への応用-魚類の造血幹/前駆細胞の同定- (2010)
  • Author(s): 森友忠昭, 小林功, 片倉文彦, and山口卓哉
  • Journal Title: 日本血栓止血学会誌 Volume: 21 Pages: 42-44
  • Peer Reviewed
• [Journal Article] Clonal growth of carp(Cyprinus carpio) T cells in vitro (2010)
  • Author(s): Yamaguchi, T., Katakura, F., Shitanda, S., Niida, Y., Toda, H., Ohtani, M., Yabu, T., Suetake, H., Moritomo, T., and Nakanishi, T
  • Journal Title: Developmental & Comparative Immunology Volume: 35 Pages: 193-202
  • Peer Reviewed
• [Journal Article] Bone marrow biopsy from the flipper of a dolphin (2010)
  • Author(s): Itou, T., Koie, H., Segawa, T., Kato, M., Yanagisawa, M., Ueda, K., Kuwano, R., Suzuki, M., Moritomo, T., and Sakai, T
  • Journal Title: The Veterinary Journal Volume: 185 Pages: 216-217
  • Peer Reviewed
• [Journal Article] Demonstration of T cell and macrophage progenitors in carp(Cyprinus carpio) kidney hematopoietic tissues. Development of clonal assay system for carp hematopoietic cells (2010)
  • Author(s): Katakura, F., Yamaguchi, T., Yoshida, M., Moritomo, T., and Nakanishi, T
  • Journal Title: Developmental & Comparative Immunology Volume: 34 Pages: 685-689
  • Peer Reviewed
• [Journal Article] Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoietic stem cells (2010)
  • Author(s): Kobayashi, I., Ono, H., Moritomo, T., Kano, K., Nakanishi, T., and Suda, T
  • Journal Title: Blood Volume: 115 Pages: e1-e9
  • Peer Reviewed
• [Journal Article] Co-culture of carp(Cyprinus carpio) kidney haematopoietic cells with feeder cells resulting in long-term proliferation of T-cell lineages (2009)
  • Author(s): Katakura, F., Takizawa, F., Yoshida, M., Yamaguchi, T., Araki, K., Tomana, M., Nakao, M., Moritomo, T., and Nakanishi, T
  • Journal Title: Veterinary immunology and immunopathology Volume: 131 Pages: 127-136
  • Peer Reviewed
• [Journal Article] 魚類における造血幹細胞の同定 (2009)
  • Author(s): 小林功・森友忠昭
  • Journal Title: 血液・腫瘍科 Volume: 58 Pages: 449-454
  • Peer Reviewed
• [Presentation] コイ栓球造血におけるTPOとkitlaの作用 (2012)
  • Author(s): 杉江勇二・片倉文彦・藪健史・加藤尚志・森友忠昭・中西照幸
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-29
• [Presentation] コイTh2様細胞のクローン性増殖 (2012)
  • Author(s): 山口卓哉・片倉文彦・Johannes Martinus Dijkstra・森友忠昭・中西照幸
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-29
• [Presentation] コイIL-4/13BのB細胞培養に対する効果 (2012)
  • Author(s): 宮田俊輔・山口卓哉・片倉文彦・柴崎康宏・藪健史・森友忠昭・中西照幸
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-29
• [Presentation] Clonal growth of Carp(Cyprinus carpio) helper T cells-Identification of Th2-like cells (2012)
  • Author(s): Tadaaki Moritomo
  • Organizer: 2012 Taiwan. Japan Symposium on Emerging Trends in Aquatic Animal Immunity and Aquaculture Biotechnology
  • Place of Presentation: National Cheng Kung University
  • Year and Date: 2012-02-22
• [Presentation] コイTPOの遺伝子クローニングおよび性状解析 (2011)
  • Author(s): 林潔高・片倉文彦・藪健史・森友忠昭・中西照幸
  • Organizer: 第23回日本比較免疫学会
  • Place of Presentation: 海洋開発研究機構
  • Year and Date: 2011-08-21
• [Presentation] 魚類の造血機構解明を目的としたコイkit ligand Aの機能解析 (2011)
  • Author(s): 尻無浜由希・片倉文彦・藪健史・森友忠昭・中西照幸
  • Organizer: 第23回日本比較免疫学会
  • Place of Presentation: 海洋開発研究機構
  • Year and Date: 2011-08-21
• [Presentation] In vitroにおけるコイCD4+T細胞のクローン性増殖 (2011)
  • Author(s): 山口卓哉・片倉文彦・森友忠昭・中西照幸
  • Organizer: 日本生体防御学会
  • Place of Presentation: 那覇市ぶんかテンブス館
  • Year and Date: 2011-06-29
• [Presentation] 魚類の造血幹/前駆細胞の分離と同定 (2011)
  • Author(s): 森友忠昭
  • Organizer: 日本大学幹細胞研究フォーラム
  • Place of Presentation: 日本大学会館
  • Year and Date: 2011-01-22
• [Presentation] Clonal growth of carp(Cyprinus carpio) CD4 single-positive αβT cells in vitro (2010)
  • Author(s): Takuya Yamaguchi, Fumihiko Katakura, Tadaaki Moritomo
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, Florida, USA
  • Year and Date: 2010-09-05
• [Presentation] コイ末梢血白血球の培養におけるBリンパ球の増殖 (2010)
  • Author(s): 仁井田祥光・片倉文彦・山口卓哉・森友忠昭・中西照幸
  • Place of Presentation: 九州大学西新プラザ第会議室
  • Year and Date: 2010-08-02
• [Presentation] クローンギンブナを用いたエピジェネティクス研究モデルの構築 (2010)
  • Author(s): 森友忠昭・伊佐拓也・大野早苗
  • Organizer: 日本大学先端バイオフォーラム
  • Place of Presentation: 日本大学会館
  • Year and Date: 2010-02-23
• [Presentation] コイ造血細胞の培養-増殖したT細胞のクローン性と分化段階の解析- (2009)
  • Author(s): 山口卓哉、片倉文彦、四反田聡、吉田美幸、森友忠昭、中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-08-03
• [Presentation] 魚類の造血幹/前駆細胞の分離と同定 (2009)
  • Author(s): 森友忠昭・関谷麻優子・小林功・片倉文彦・吉田美幸・山口卓哉・高野晃一
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-08-03
• [Presentation] LONG-TERM PROLIFERATION OF COMMON CARP(Cyprinus carpio) CD4+αβT-CELLS IN CULTURE (2009)
  • Author(s): T. Yamaguchi, F. Katakura, M. Yoshida, M. Ohtani, T. Moritomo and T. Nakanishi
  • Organizer: 11^<th> International Society of Developmental and Comparative Immunology
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2009-06-27
• [Presentation] DEVELOPMENT OF THE SINGLE-CELL COLONY-FORMING ASSAY SYSTEM : CHARACTERIZATION OF CARP(CYPRINUS CARPIO) KIDNEY HEMATOPOIETIC PROGENITOR CELLS (2009)
  • Author(s): F. Katakura, M. Yoshida, T. Yamaguchi, T. Moritomo, and T. Nakanishi
  • Organizer: 11th International Society of Developmental and Comparative Immunology
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2009-06-27
• [Book] 魚病学 (2012)
  • Author(s): 児玉洋・川本恵子・森友忠昭・和田新平
  • Total Pages: 150
  • Publisher: 緑書房
• [Remarks]
  • URL: http://hp.brs.nihon-u.ac.jp/～moritomo/index.htm