2011 Fiscal Year Final Research Report
Elucidation of antigenic cross-reactivity mechanism in fish roe allergy
| Project/Area Number |
21580245
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAEKI Hiroki 北海道大学, 大学院・水産科学研究院, 教授 (90250505)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Akihiko 北海道大学, 大学院・水産科学研究院, 特任教授 (40091483)
SHIMIZU Yutaka 北海道大学, 大学院・水産科学研究院, 技術職員 (00374629)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 魚卵 / 食物アレルギー / アレルゲン / β'-コンポーネント |
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| Research Abstract |
Allergen analysis of major allergic yolk protein, β'-component (β'-c) was examined in this study and the results were as follows: (1) Five kinds of IgE sequential epitopes in β'-c were identified by overlapped peptide mapping and ELISA. The three kinds of the IgE-binding sites seem to be common epitopes of fish roe, because they were located in high homologous regions of fish β'-cs. (2) Six kinds of β'-c isoforms were prepared from mullet roe and they showed IgE cross-reactivity with salmon β'-c. (3) Vitellogenin subfragment over 1 μg/g were observed in matured salmon muscle and they showed β'-c-specific IgE-binding ability, suggesting food intake of fish meat may induce sensitization to fish roe allergy.
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Research Products
(6 results)
