Microbiological study on fermented mare's milk

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580331
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Okayama University
• Principal Investigator: MIYAMOTO Taku 岡山大学, 大学院・自然科学研究科, 教授 (00093708)
• Project Period (FY): 2009 – 2011
• Keywords: 馬乳酒 / 微生物フローラ / DGGE法 / 乳酸菌 / 酵母 / 共生作用 / 拮抗作用 / モンゴル地域

Research Abstract

We investigated the microbiota of lactic acid bacteria(LAB) and yeasts using conventional plate method and DGGE(denaturing gradient gel electrophoresis) analysis from fermented mare's milk, airag that was collected from different nomadic families in Mongolia and Inner Mongolia, China. Mutual symbiosis between LAB and yeast strains isolated from airag was observed. These possible positive interactions may be important for the development of LAB-yeast strains as starter cultures. We also isolated a bacteriocin-producing Leuconostoc strain from airag.

Research Products

• [Journal Article] Interaction between lactic acid bacteria and yeasts in airag, an alcoholic fermented milk (2012)
  • Author(s): Sudan, Wulijideligen, Arakawa, K., Miyamoto, M. and Miyamoto, T.
  • Journal Title: Animal Science Journal Volume: 83(in press)
  • Peer Reviewed
• [Journal Article] Production of bacteriocin by Leucono-stoc mesenteroides 406 isolated from Mongolian fermented mare's milk, airag (2012)
  • Author(s): Wulijideligen, Asahina, T., Hara, K., Arakawa, K., Nakano, H. and Miyamoto, T.
  • Journal Title: Animal Science Journal Volume: 83
  • DOI: doi:10.1111/j.1740-0929.2012.01010
  • Peer Reviewed
• [Journal Article] Screening and identification of lactic acid bacteria from airag for antifungal activity (2011)
  • Author(s): Sudan, Wulijideligen and Miyamoto, T.
  • Journal Title: Journal of Animal and Veterinary Advances Volume: 10 Pages: 2751-2757
  • Peer Reviewed
• [Journal Article] Isolation and identifi-cation of yeasts in chigee, fermented mare's milk, a traditional drink of Inner Mongolia, China (2010)
  • Author(s): Sudan, Baiyu, Shuangquan, Wulijideligen and Miyamoto, T.
  • Journal Title: Milk Science Volume: 59 Pages: 231-236
  • Peer Reviewed
• [Journal Article] Denaturing gradientgel electrophoresis analysis of lactic acid bacteria and yeasts in traditional Mongolian fermented milk (2010)
  • Author(s): Miyamoto, M., Seto, Y., Nakajima, H., Burenjargal, S., Gombojav, A., Demberel, S. and Miyamoto, T.
  • Journal Title: Food Science and Technology Research Volume: 16 Pages: 319-326
  • Peer Reviewed
• [Presentation] モンゴル国アイラグの微生物フローラについて (2012)
  • Author(s): Bolormaa, T., 他4名
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋大学東山キャンパス(名古屋市)
  • Year and Date: 2012-03-29
• [Presentation] 乳酸菌の機能性探索と利用に関する研究 (2011)
  • Author(s): 宮本拓
  • Organizer: 中国四国農林水産・食品先進技術研究会
  • Place of Presentation: 岡山コンベンションセンター(岡山市)
  • Year and Date: 2011-12-16
• [Presentation] 馬乳酒起源乳酸菌における生理機能の探索について (2011)
  • Author(s): 池田裕美, 他5名
  • Organizer: 平成23年度酪農科学シンポジウム
  • Place of Presentation: フォレスト仙台(仙台市)
  • Year and Date: 2011-09-22
• [Presentation] アルコール発酵乳アイラグを構成する乳酸菌と酵母の共生作用について (2011)
  • Author(s): 蘇敦, 他3名
  • Organizer: 平成23年度酪農科学シンポジウム
  • Place of Presentation: フォレスト仙台(仙台市)
  • Year and Date: 2011-09-22
• [Presentation] モンゴル国の馬乳酒~とくに乳酸菌フローラについて～~ (2009)
  • Author(s): 宮本真理, 他7名
  • Organizer: 日本モンゴル学会
  • Place of Presentation: 岡山大学50周年記念館(岡山市)
  • Year and Date: 2009-11-21
• [Book] ミルクの事典 (2009)
  • Author(s): 宮本拓
  • Total Pages: 282-283
  • Publisher: 朝倉書店