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2011 Fiscal Year Final Research Report

Functional analysis on EphB and ephrin-B expressing in gastric mucosa and their expression behavior in gastric ulcer and cancer

Research Project

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Project/Area Number 21580367
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionOsaka Prefecture University

Principal Investigator

OGAWA Kazushige  大阪府立大学, 生命環境科学研究科, 教授 (60231221)

Co-Investigator(Renkei-kenkyūsha) NAKAJIMA Takayuki  大阪府立大学, 生命環境科学研究科, 准教授 (30333644)
ISHII Maki  大阪府立大学, 生命環境科学研究科, 助教 (50415535)
Research Collaborator ELENA Pasquale  Sanford-Burnham Medical Research Institute(La Jolla, USA), 教授
Project Period (FY) 2009 – 2011
KeywordsEphB / ephrin-B / 胃粘膜上皮 / 細胞構築 / 境界形成
Research Abstract

Several EphB receptors and ephrin-Bs were detected in the gastric mucosa of the adult rodent stomach by RT-PCR amplification. Immunostaining showed complementary expression patterns, with EphB receptors preferentially expressed in the deeper regions and ephrin-Bs in the superficial regions of the gastric units. In a mouse ulcer model EphB2 expression was upregulated in the regenerating epithelium. EphB signaling in primary gastric epithelial cells promoted cell retraction and repulsion at least in part through RhoA activation. Based on these findings the author propose that the EphB-positive progeny of gastric stem cells migrates from the isthmus towards the bottom of the gastric glands due to repulsive signals arising from contact with ephrin-Bs, which are preferentially expressed in the more superficial regions of the isthmus and gastric pits. In a rat gastric cancer model EphB2 and ephrin-B were co-localized in Ki67-positive proliferative cells forming aberrant gastric units and gastric cancer cells were simultaneously expressed EphB receptors and ephrin-B ligands. These findings suggest that simultaneous expressions of both EphB receptors and ephrin-B indicates an characteristic of gastric cancer cells.

  • Research Products

    (13 results)

All 2012 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 2 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] マウスとラットの胃の無腺部と噴門部粘膜上皮の境界領域に発現するEphB2とephrin-B12012

    • Author(s)
      居蔵泰隆, 石井万幾, 中島崇行, 小川和重
    • Journal Title

      解剖学雑誌

      Volume: 87巻(印刷中)

  • [Journal Article] Complementary expression and repulsive signaling suggest that EphB receptors and ephrin-B ligands control cell positioning in the gastric epithelium2011

    • Author(s)
      Ogawa K, Takemoto N, Ishii M, Mueller I, Pasquale E B, Nakajima T.
    • Journal Title

      Histochem Cell Biol

      Volume: 136 Pages: 617-636

    • Peer Reviewed
  • [Journal Article] Complementary expression of EphB receptors and ephrin-B ligands in the pyloric and duodenal epithelium of adult mice2011

    • Author(s)
      Ishii M, Nakajima T, Ogawa K.
    • Journal Title

      Histochem Cell Biol

      Volume: 136 Pages: 345-356

    • Peer Reviewed
  • [Journal Article] 再生および異常増殖胃粘膜上皮のEphBとephrin-B2010

    • Author(s)
      内山慎太郎, 石井万幾, 中島崇行, 小川和重
    • Journal Title

      解剖学雑誌

      Volume: 85(2) Pages: 74

  • [Journal Article] EphBはRhoAを活性化して初代培養胃腺細胞の膜退縮を誘導する2009

    • Author(s)
      小川和重, 内山慎太郎, ミュラー樹, 中島崇行
    • Journal Title

      日本解剖学雑誌

      Volume: 84(2) Pages: 63

  • [Presentation] EphB2 and ephrin-B1 expression in the boundary between the glandular and nonglandular epithelium of the rodent stomach2012

    • Author(s)
      Kazushige Ogawa, Yasutaka Igura, Yuta Hayashi, Noritaka Saeki, Maki Ishii and Takayuki Nakajima
    • Organizer
      14th International Congress on Histochemistry and Cytochemistry
    • Place of Presentation
      Kyoto, Japan(Kyoto International Conference Center)
    • Year and Date
      20120826-29
  • [Presentation] マウスとラットの胃の無腺部と噴門部粘膜上皮の境界領域に発現するEphB2とephrin-B12011

    • Author(s)
      居蔵泰隆, 石井万幾, 中島崇行, 小川和重
    • Organizer
      第87回日本解剖学会近畿支部学術集会
    • Place of Presentation
      西宮市(兵庫医科大学)
    • Year and Date
      2011-12-03
  • [Presentation] 無腺部と胃腺部の境界領域の胃粘膜上皮に発現するEphB2とephrin-B12011

    • Author(s)
      居蔵泰隆, 石井万幾, 中島崇行, 小川和重
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      堺市(大阪府立大学)
    • Year and Date
      2011-09-20
  • [Presentation] 再生および異常増殖胃粘膜上皮のEphBとephrin-B発現2009

    • Author(s)
      内山慎太郎, 石井万幾, 中島崇行, 小川和重
    • Organizer
      日本解剖学会第85回近畿支部学術集会
    • Place of Presentation
      橿原市(奈良県立医科大学)
    • Year and Date
      2009-11-28
  • [Presentation] EphB/ephrin-B may regulate cell positioning in the gastric epithelium2009

    • Author(s)
      Kazushige Ogawa, Natsuki Takemoto, Maki Ishii, Takayuki Nakajima
    • Organizer
      第50回日本組織細胞化学会
    • Place of Presentation
      大津市(ピアザ淡海滋賀県立県民交流センター)
    • Year and Date
      2009-09-27
  • [Presentation] 胃潰瘍および胃癌モデルラットのEphBとephrin-B発現2009

    • Author(s)
      内山慎太郎, 石井万幾, 中島崇行, 小川和重
    • Organizer
      第148回日本獣医学会総会
    • Place of Presentation
      鳥取市(とりぎん文化会館)
    • Year and Date
      2009-09-25
  • [Presentation] 組織の細胞構築を制御する分子Ephとephrin : EphB/ephrin-Bは胃粘膜上皮のポジショニングを制御する分子システムであるか?2009

    • Author(s)
      小川和重
    • Organizer
      第147回日本獣医学会総会生理・生化学シンポジウム
    • Place of Presentation
      宇都宮市(栃木県総合文化センター)
    • Year and Date
      2009-04-04
  • [Remarks]

    • URL

      http://www.vet.osakafu-u.ac.jp/anat/anat.html

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Published: 2013-07-31  

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