2011 Fiscal Year Final Research Report
Study on development of sugar feeding method that prevents animal botulism
| Project/Area Number |
21580383
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
NIWA Koichi 東京農業大学, 生物産業学部, 教授 (20301012)
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| Co-Investigator(Renkei-kenkyūsha) |
OHYAMA Tohru 東京農業大学, 生物産業学部, 教授 (60318178)
WATANABE Toshihiro 東京農業大学, 生物産業学部, 教授 (80175695)
OGURI Suguru 東京農業大学, 生物産業学部, 教授 (70277250)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ボツリヌス症 / ボツリヌス毒素 / 小腸上皮細胞 / 糖鎖 / 吸収 / 疾病予防 |
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| Research Abstract |
To establish the preventive against bovine botulism, the effects of sugar on the transport of botulinum toxin complex(L-TC) via cultured intestinal epithelial cells were examined. L-TC permeated the cell monolayers, and the permeation were potently inhibited by sialic acid in the cell culture medium, indicating that the binding of botulinum toxins to cell surface sialic acid leads to their transcytosis through intestinal epithelial cells. Present results imply that bovine botulism can be prevented by an addition of sialic acid to feed
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[Journal Article] Characterization of sugar recognition by the toxin complex produced by the Clostridium botulinum serotype C variant strain Yoichi2011
Author(s)
Matsuo, T., Miyata, K., Inui, K., Ito, H., Horiuchi, R., Suzuki, T., Yoneyama, T., Oguma, K., Niwa, K., Watanabe, T. and Ohyama, T.
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Journal Title
FEMS Immunol. Med. Microbiol
Volume: 63
Pages: 35-43
DOI
Peer Reviewed
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[Journal Article] HA-33 facilitates transport of the serotype D botulinum toxin across a rat intestinal epithelial cell monolayer2011
Author(s)
Ito, H., Sagane, Y., Miyata, K., Inui, K., Matsuo, T., Horiuchi, R., Ikeda, T., Suzuki, T., Hasegawa, K., Kouguchi, H., Oguma, K., Niwa, K., Ohyama, T. and Watanabe, T.
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Journal Title
FEMS Immunol. Med. Microbiol
Volume: 61
Pages: 323-331
DOI
Peer Reviewed
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[Journal Article] Involvement of sialic acid in transport of serotype C1 botulinum toxins through rat intestinal cells2010
Author(s)
Inui, K., Ito, H., Miyata, K., Matsuo, T., Horiuchi, R., Ikeda, T., Watanabe, T., Ohyanma, T. and Niwa, K.
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Journal Title
J. Vet. Med. Sci
Volume: 72
Pages: 1251-1255
URL
Peer Reviewed
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[Journal Article] Sialic acid-dependent binding and transcytosis of serotype D botulinum neurotoxin and toxin complex in rat intestinal epithelial cells2010
Author(s)
Niwa, K., Yoneyama, T., Ito, H., Taira, M., Chikai, T., Kouguchi, H., Suzuki, T., Hasegawa, K., Miyata, K., Inui, K., Ikeda, T., Watanabe, T. and Ohyama, T.
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Journal Title
Vet. Microbiol
Volume: 141
Pages: 312-320
DOI
Peer Reviewed
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