2011 Fiscal Year Final Research Report
Motional linkage between voltage sensor and ion gate in voltage-dependent ion channels
| Project/Area Number |
21590040
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OSAWA Masanori 東京大学, 大学院・薬学系研究科, 助教 (60361606)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Ichio 東京大学, 大学院・薬学系研究科, 教授 (70196476)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 構造生物学 |
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| Research Abstract |
Voltage-dependent ion channels consist of a voltage-sensor domain(VSD) and a pore domain possessing the ion permeation pathway. This study demonstrated how VSD changes its conformation in a voltage-dependent manner, by a disulfide-locking method using a series of double cysteine mutants. In addition, the binding mode between VSD and its inhibiting toxin, analyzed by NMR, revealed the inhibition mechanism of the toxin.
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