Induction mechanism of imposex in marine gastropods by retinoids

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590143
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Mukogawa Women's University
• Principal Investigator: NISHIKAWA Jun-ichi 武庫川女子大学, 薬学部, 教授 (90218131)
• Project Period (FY): 2009 – 2011
• Keywords: 環境化学

Research Abstract

Organotin compounds have been widely used as antifouling pints for ships and fishing nets since the 1960s and have thus released into marine environments. Aquatic invertebrates, particularly marine gastropods, are extremely sensitive to organotin compounds such as tributyltin(TBT) and triphenyltin(TPT). So far, very low-concentartion of TBT or TPT have been shown to induce imposex(superimposition of male genitalia on female) in marine gastropods. Although the inposex induction mechanism has been controversial for many years, we have reported that TBT and TPT are potent and efficacious activators of retinoid X receptor(RXR), and the natural ligand of RXR, 9-cis retinoic acid, induces imposex. However, in vertebrates, all-trans retinoic acid is well-known morphogen important for axial patterning and organ formation in early development. In this study, we analyze the molluscan receptor for all-trans retinoic acid in the view of the evolutionary history of retinoid machinery.

Research Products

• [Journal Article] Effect of lipopoly saccharide on the xenobiotic nduced expression and activity of hepatic cytochrome P450 in mice (2012)
  • Author(s): Moriya N, Kataoka H, Fujino H, Nishikawa J, Kugawa F
  • Journal Title: Biol. Pharm. Bull Volume: 35 Pages: 473-480
  • Peer Reviewed
• [Journal Article] Inositol phosphate kinase Vip1p interacts with histone chaperone Asf1p in Sacchromyces cerevisiae (2012)
  • Author(s): Osada S, Kageyama K, Ohnishi Y, Nishikawa J, Nishihara T, Imagawa M
  • Journal Title: Mol. Biol. Rep. Volume: 39 Pages: 4989-4996
  • Peer Reviewed
• [Journal Article] Induction of integrinβ3 in PGE2-stimulated adhesion of mastocytoma P-815 cells to the Arg-Gly-Asp-enriched fragment of fibronectin (2011)
  • Author(s): Okada Y, Nishikawa J, Semma M, Ichikawa A
  • Journal Title: Biochem. Pharmacol Volume: 81 Pages: 866-872
  • Peer Reviewed
• [Journal Article] Contamination with retinoic acid receptor agonists in two rivers in the Kinki region of Japan (2010)
  • Author(s): Inoue D, Nakama K, Sawada K, Watanabe T, Takagi M, Sei K, Yang M, Hirotsuji J, Hu J, Nishikawa J, Nakanishi T, Ike M
  • Journal Title: Water Res Volume: 44 Pages: 2409-2418
  • Peer Reviewed
• [Journal Article] Site-directed mutagenesis of rat thioltransferase : effects of essential cysteine residues for the protection against oxidative stress (2010)
  • Author(s): Terada T, Okamoto K, Nishikawa J, Miura T, Nishinaka T, Nishihara T
  • Journal Title: J. Biochem. Mol. Toxicol Volume: 24 Pages: 60-65
  • Peer Reviewed
• [Journal Article] Identification of retinoic acid receptor agonists in sewage treatment plants (2009)
  • Author(s): Zhen H, Wu X, Hu J, Xiao Y, Yang M, Hirotsuji J, Nishikawa J, Nakanishi T, Ike M
  • Journal Title: Environ. Sci. Technol Volume: 43 Pages: 6611-6616
  • Peer Reviewed
• [Journal Article] Structuredependent activation of peroxisome proliferator-activated receptor gamma by orgnotin compounds (2009)
  • Author(s): Hiromori Y, Nishikawa J, Yoshida I, Nagase H, Nakanishi T
  • Journal Title: Chemical. Biol. Interact Volume: 180 Pages: 238-244
  • Peer Reviewed