Analysis of molecules involved in neuronal polarity formation : a study using neuronal specific knockout mice

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka University
• Principal Investigator: HARADA Reiko 大阪大学, 医学系・研究科, 特任講師 (40230718)
• Co-Investigator(Renkei-kenkyūsha): HARADA Akihiro 大阪大学, 大学院・医学系研究科, 教授 (40251441)
• Project Period (FY): 2009 – 2011
• Keywords: 神経細胞 / 極性輸送 / 軸索伸長 / ノックアウトマウス / VAMP7

Research Abstract

Various proteins are required in the establishment of neuronal polarity. In this study, we analyzed mice deficient in VAMP7, a protein proposed to be involved in axonal transport. Contrary to expectations, VAMP7 knockout mice displayed similar localization of axonal proteins in the nervous system. However, neurite outgrowth of cultured mutant hippocampal neurons was slightly affected, suggesting that VAMP7 have some role in polarized transport of axonal proteins. This work was published in Traffic, an international journal of intracellular transport.

Research Products

• [Journal Article] The role of VAMP7/ TI-VAMP in cell polarity and lysosomal exocytosis in vivo (2011)
  • Author(s): Sato M, Yoshimura S, Hirai R, Goto A, Kunii M, Atik N, Sato T, Sato K, Harada R, Shimada J, Hatabu T, Yorifuji H, Harada A.
  • Journal Title: Traffic Volume: 12 Pages: 1383-1393
  • Peer Reviewed
• [Journal Article] p31 deficiency influences endoplasmic reticulum tubular morphology and cell survival (2009)
  • Author(s): Uemura T, Sato T, Aoki T, Yamamoto A, Okada T, Hirai R, Harada R, Mori K, Tagaya M, Harada A
  • Journal Title: Mol Cell Biol Volume: 29 Pages: 1869-1881
  • Peer Reviewed
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/acb/