2011 Fiscal Year Final Research Report
Study on the mechanisms of STAT3-dependent transcriptional elongation
| Project/Area Number |
21590317
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Osaka City University |
|---|
Principal Investigator |
NAKAJIMA Koichi 大阪市立大学, 大学院・医学研究科, 教授 (00227787)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | STAT3 / 転写伸長 / キナーゼ / チロシンフォスファターゼ |
|---|
| Research Abstract |
We studied 1) the mechanisms of STAT3-dependent transcriptional activation of its target genes, especially at the elongation level and 2) the role of the STAT3 Ser727 phosphorylation in the gene regulation. We found that STAT3 recruits CDK9, CDK12 and CDK13 as well as super elongation complex(SEC) to its target genes including socs3 gene promoter and 3' ORF. It is likely that multiple Pol II CTD kinases are involved in the STAT3-regulated gene elongation. The role of SEC seemed to vary depending on the target genes. We found that phosphorylation of Ser727 of STAT3 determines the duration of STAT3 activity largely through nuclear protein tyrosine phosphatase TC45.
|
