2011 Fiscal Year Final Research Report
Study of Shiga toxin productions us ing in vivo analysis
| Project/Area Number |
21590478
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
SHIMIZU Takeshi 千葉大学, 大学院・医学研究院, 准教授 (70312840)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 腸管出血性大腸菌 / 志賀毒素 / 毒素産生 / 病原性 |
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| Research Abstract |
A novel chromosome-plasmid hybrid bioluminescent reporter system (C-P reporter system) utilizing Photorhabdus luminescens luxCDABE genes has been constructed to monitor the expression of Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2) in enterohaemorrhagic Escherichia coli (EHEC) in real time. Using these reporter strains, we examined the profiles of Stx1 and Stx2 expression in EHEC. We found that production of both Stx1 and Stx2 in EHEC was enhanced upon contact with intestinal epithelial cells and within macrophages.
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