Effects of fat in diets on heterozygous mutants of carnitine-deficient mice

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590704
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Kagoshima University
• Principal Investigator: HORIUCHI Masahisa 鹿児島大学, 医歯学総合研究科, 准教授 (50264403)
• Co-Investigator(Kenkyū-buntansha): TAKEUCHI Toru 鹿児島大学, 医歯学総合研究科, 教授 (00188161) ; ASAKAWA Akihiro 鹿児島大学, 医歯学総合研究科, 准教授 (10452947)
• Project Period (FY): 2009 – 2011
• Keywords: カルニチン / ヘテロ接合体 / 脂肪酸代謝異常 / 肥満 / レプチン抵抗性 / 高脂肪食

Research Abstract

JVS mice, an animal model for systemic carnitine deficiency, were examined in effects of high-fat diets on weight. Male heterozygous JVS mice showed higher body weight due to higher food intake, in association with higher leptin in blood, compared with wild type of mice. Additionally, male heterozygous JVS mice showed higher AGRP and lower POMC mRNAs in the hypothalamus, indicating that heterozygotes may have leptin resistant state in central nervous system. The low carnitine levels in brain may be involved in the central leptin resistance, because carnitine ingestion from periphery was not effective.

Research Products

• [Journal Article] Low-protein diet improves blood and urinary glucose levels and renal manifestations of diabetes in C57BLKS-db/db mice (2012)
  • Author(s): Arimura E, Horiuchi M, Kawaguchi H, Miyoshi N, Aoyama K, Takeuchi T
  • Journal Title: Eur J Nutr Volume: (in press)
  • Peer Reviewed
• [Journal Article] Acupuncture improves sleep conditions of minipigs representing diurnal animals through an anatomically similar point to the acupoint(GV20) effective for humans (2012)
  • Author(s): Takeishi K-I, Horiuchi M, Kawaguchi H, Deguchi Y, Izumi H, Arimura E, Kuchiiwa S, Tanimoto A, Takeuchi T
  • Journal Title: Evid Based Complement Alternat Med Volume: 2012 Pages: 472982
  • Peer Reviewed
• [Journal Article] Benzene metabolite, 1, 2, 4-benzenetriol, induced halogenated DNA and tyrosine representing halogenative stress in human myeloid cell line HL-60 (2012)
  • Author(s): Nishikawa T, Miyahara E, Horiuchi M, Izumo K, Okamoto Y, Kawai Y, Kawano Y, Takeuchi T.
  • Journal Title: Environ Health Perspect Volume: 120 Pages: 62-67
  • Peer Reviewed
• [Journal Article] Long-term voluntary exercise, representing habitual exercise, lowers visceral fat and alters plasma amino acid levels in mice (2011)
  • Author(s): Takeshita H, Horiuchi M, Izumo K, Kawaguchi H, Arimura E, Aoyama K, Takeuchi T
  • Journal Title: Environ Health Prev Med Volume: 4
  • Peer Reviewed
• [Journal Article] Effect of exercise and high-fat diet on plasma adiponectin and nesfatin levels in mice (2011)
  • Author(s): Chaolu H, Asakawa H, Ushikai M, Li YX, Cheng KC, Li JB, Zoshiki T, Terashi M, Tanaka C, Atsuchi K, Sakoguchi T, Tsai M, Amitani H, Horiuchi M, Takeuchi T, Inui A
  • Journal Title: Exp Ther Med Volume: 2 Pages: 369-373
  • Peer Reviewed
• [Journal Article] Effect of habitual exercise on renal carcinogenesis by ferric nitrilotriacetate (2011)
  • Author(s): Kato T, Kawaguchi H, Miyoshi N, Aoyama K, Komatsu M, Horiuchi M, Yoshida H, Takeuchi T.
  • Journal Title: Environ Health Prev Med Volume: 16 Pages: 232-238
  • Peer Reviewed
• [Journal Article] Benzene induces cytotoxicity without metabolic activation (2011)
  • Author(s): Nishikawa T, Izumo K, Miyahara E, Horiuchi M, Okamoto Y, Kawano Y, Takeuchi T.
  • Journal Title: J Occup Health Volume: 53 Pages: 84-92
  • Peer Reviewed
• [Journal Article] Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms (2011)
  • Author(s): Miyahara E, Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda H, Takeuchi M, Komatsu M, Aoyama K, Horiuchi M, Takeuchi T
  • Journal Title: FEMS Microbiol Lett Volume: 317 Pages: 109-116
  • Peer Reviewed
• [Journal Article] Induction of PDK4 in the heart muscle of JVS mice, an animal model of systemic carnitine deficiency, does not appear to reduce glucose utilization by the heart (2011)
  • Author(s): Ushikai M, Horiuchi M, Kobayashi K, Matuda S, Inui A, Takeuchi T, Saheki T
  • Journal Title: Mol Genet Metab Volume: 102 Pages: 349-355
  • Peer Reviewed
• [Journal Article] Novel microminipig model of atherosclerosis by high fat and high cholesterol diet,established in Japan (2010)
  • Author(s): Miyoshi N, Horiuchi M, Inokuchi Y, Miyamoto Y, Miura N, Tokunaga S, Fujiki M, Izumi Y, Miyajima H, Nagata R, Misumi K, Takeuchi T, Tanimoto A, Yasuda N, Yoshida H, Kawaguchi H.
  • Journal Title: in vivo Volume: 24 Pages: 671-680
  • Peer Reviewed
• [Journal Article] Early neonatal onset carbamoyl-phosphate synthase 1 deficiency treated with continuous hemodiafiltration and early living-related liver transplantation (2009)
  • Author(s): Ishida T, Hiroma T, Hashikura Y, Horiuchi M, Kobayashi K, Nakamura T.
  • Journal Title: Pediatr Int Volume: 51 Pages: 409-410
  • Peer Reviewed
• [Journal Article] Failure of the feeding response to fasting in carnitine-deficient juvenile visceral steatosis(JVS) mice : involvement of defective acyl-ghrelin secretion and enhanced corticotropin-releasing factor signaling in the hypothalamus (2009)
  • Author(s): Sakoguchi T, Horiuchi M, Asakawa A, Ushikai M, Yoshida G, Fujimiya M, Kato I, Nakazato M, Takeuchi T, Saheki T, Inui A.
  • Journal Title: Biochim Biophys Acta(Molecular Basis of Disease) Volume: 1792 Pages: 1087-1093
  • Peer Reviewed
• [Journal Article] Dehyderoepiandrosterone increased oxidative stress in a human cell line during differentiation (2009)
  • Author(s): Izumo K, Horiuchi M, Komatsu M, Aoyama K, Bandow K, Matsuguchi T, Takeuchi M, Takeuchi T.
  • Journal Title: Free Radic Res Volume: 43 Pages: 922-931
  • Peer Reviewed
• [Presentation] 世界最小ミニブタの産業医学研究への展開 (2011)
  • Author(s): 堀内正久
  • Organizer: 日本産業衛生学会九州地方会
  • Place of Presentation: 佐賀市
  • Year and Date: 2011-07-01
• [Presentation] 世界最小ミニブタの栄養学実験への応用 (2011)
  • Author(s): 堀内正久、川口博明
  • Organizer: 日本病態栄養学会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-01-16
• [Presentation] 習慣的な自発運動の肺炎予防効果に関する実験的研究:日の遺伝子発現変化からの考察 (2009)
  • Author(s): 堀内正久、出雲公子、竹下温子、川口博明、青山公治、竹内亨
  • Organizer: 第7回日本予防医学会学術総会
  • Place of Presentation: 千葉市
  • Year and Date: 2009-12-12
• [Presentation] 大学における労働衛生管理の現状 (2009)
  • Author(s): 堀内正久
  • Organizer: 日本産業衛生学会九州地方会
  • Place of Presentation: 那覇市
  • Year and Date: 2009-06-27