2011 Fiscal Year Final Research Report
Identification of hepatic encephalopathy induced proteins using by a bio-artificial liver and toxin receptor knock out hepatitis model mice
| Project/Area Number |
21590862
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Naofumi 東京慈恵会医科大学, 医学部, 教授 (80138742)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 肝臓学 / 肝性脳症 / バイオ人工肝臓 / 肝炎モデルマウス |
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| Research Abstract |
Identification of unknown hepatic encephalopathy induced substances to cause the "rapid cerebral edema" in fulminant hepatitis is required in development of the treatment and diagnosis of fatal hepatic encephalopathy. The main purpose of this study is identification of brain astrocytes damage inducing plasma proteins. A candidate of fatal hepatic encephalopathy inducing proteins, temporary named protein A, was able to identify by proteomic analysis. Protein A induced the cytotoxicity to cultured astrocytes. Hepatic encephalopathy was induced in the TRECK hepatitis model mice. In addition, we could observe diffuse astrocyte swelling in acute hepatic encephalopathy by DWI on MRI, as a decrease in the ADC. We will study whether protein A induce hepatic encephalopathy in vivo model and clinical cases in next step, and promote the treatment for fatal encephalopathy.
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