Identification and function analysis of micro RNAs in progression of heart failure

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590914
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Fujita Health University
• Principal Investigator: IZAWA Hideo 藤田保健衛生大学, 医学部, 教授 (80402569)
• Co-Investigator(Kenkyū-buntansha): OZAKI Yukio 藤田保健衛生大学, 医学部, 教授 (50298569) ; CHENG Xian wu 名古屋大学, 医学部, 講師 (30378228)
• Co-Investigator(Renkei-kenkyūsha): CHENG Xian wu 名古屋大学, 医学部, 講師 (30378228) ; NOMURA Masanori 藤田保健衛生大学, 医学部, 教授 ; MORIMOTO Shinichiro 藤田保健衛生大学, 医学部, 教授 ; MUROHARA Toyoaki 名古屋大学, 大学院・医学系研究科, 教授 (90299503) ; HIRASHIKI Akihiro 名古屋大学, 医学部, 助教 (10418741)
• Project Period (FY): 2009 – 2011
• Keywords: 心臓病態学 / 心不全 / マイクロRNA / 心筋線維化

Research Abstract

Both myocardial fibrosis and reduce catecholamine sensitivity had been fully demonstrated to be involved in pathogenesis of heart failure. However, the molecular mechanisms that underlie myocardial fibrosis remain incompletely understood. First, we performed microarray analysis for a total of 485 miRNAs using endomyocardial biopsy specimen from 14 patients with idiopathic dilated cardiomyopathy(DCM). Out of 485 miRNAs, 7 were differentially expressed in the myocardium with reduced catecholamine sensitivity. Second, 7 miRNAs were differentially expressed significantly in 4 patients with severe myocardial fibrosis compared with 4 patients with less severe myocardial fibrosis. We demonstrated that 4 specific miRNAs could play key roles in myocardial fibrosis in patients with DCM.

Research Products

• [Journal Article] Altered microRNA expression associated with reduced catecholamine sensitivity in patients with chronic heart failure (2011)
  • Author(s): Funahashi H, Izawa H, Hirashiki A, Cheng XW, Inden Y, Nomura M, Murohara T
  • Journal Title: J Cardiol Volume: 57(3) Pages: 338-44
  • Peer Reviewed
• [Presentation] Micro RNA Could Play Key Roles in Progression of Myocardial Fibrosis in Patients with Heart Failure (2011)
  • Author(s): Kinoshita K, Izawa H, Kato Y, Yokoi H, Fujiwara W, Cheng XW, Hirashiki A, Mizoguchi Y, Ohtsuki M, Ishii J, Morimoto S, Murohara T, Ozaki Y, Nomura M
  • Organizer: American Heart Association, Scientific Sessions 2011
  • Place of Presentation: オーランド(米国)
  • Year and Date: 2011-11-14
• [Presentation] Micro RNAs could play key roles in progression of left ventricular diastolic dysfunction involved in myocardial fibrosis in patients with heart failure (2010)
  • Author(s): Izawa H, Kato Y, Yokoi H, Fujiwara W, Cheng XW, Mizoguchi Y, Morimoto S, Murohara T, Ozaki Y, Nomura M
  • Organizer: European Society of Cardiology, Congress 2010
  • Place of Presentation: ストックホルム(スエーデン)
  • Year and Date: 2010-08-29
• [Presentation] Micro RNAs Could Play a Critical Role in Progression of Diastolic Dysfunction in Mildly Symptomatic Patients with Chronic Heart Failure (2009)
  • Author(s): Izawa H, Hirashiki A, Funahashi H, Cheng XW, Kobayashi M, Harada K, Murase Y, Unno K, Shintani S, Nagata K, Ozaki Y, Yokota M, Nomura M, Murohara T
  • Organizer: American Heart Association, Scientific Sessions 2009
  • Place of Presentation: オーランド(米国)
  • Year and Date: 2009-11-17