The effects of hydrogen gas breathing on the lung injury induced by hyperoxia

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Kitasato University
• Principal Investigator: KOBAYASHI Hirosuke 北里大学, 医療衛生学部, 教授 (70153632)
• Co-Investigator(Kenkyū-buntansha): HIROSE Minoru 北里大学, 医療衛生学部, 教授 (50286382) ; KOKUBO Kenichi 北里大学, 医療衛生学部, 准教授 (20287965) ; SHINBO Toshihiro 北里大学, 医療衛生学部, 助教 (10406910) ; KUBOTA Masaru 北里大学, 医学部, 准教授 (00234500) ; HATAISHI Ryuji 北里大学, 医学部, 講師 (20276124)
• Project Period (FY): 2009 – 2011
• Keywords: 非閉塞性肺疾患癌 / 肺線維症 / 呼吸器感染症 / その他・酸素障害

Research Abstract

Survival time of mice exposed to hyperoxia became longer when the mice breathed hydrogen gas, and the leukocytes accumulation in the lungs was reduced. Hydrogen gas protected cultured human alveolar epithelial cells against antimycin A-induced oxidative stress. The level of superoxide produced by mitochondria was significantly low, and the increase in superoxide produced by antimycin A was also lower in the presence of hydrogen gas. Hydrogen gas protected lungs against hyperoxic lung injury.

Research Products

• [Journal Article] Ectopic expression of a T-box transcription factor, eomesodermin, renders CD4+Th cells cytotoxic by activating both perforin-and FasLpathways (2012)
  • Author(s): Eshima E, Chiba S, Suzuki H, Kokubo K, Kobayashi H, Iizuka M, Iwabuchia K, Shinohara N
  • Journal Title: Immunology Letters Volume: 144 Pages: 7-15
  • Peer Reviewed
• [Journal Article] Rapid deconjugation of SN. 38 glucuronide and adsorption of released free SN. 38 by intestinal microorganisms in rat (2011)
  • Author(s): Takakura A, Kurita A, Asahara T, Yokoba M, Yamamoto M, Ryuge S, Igawa S, Yasuzawa Y, Sasaki J, Kobayashi H, Masuda N
  • Journal Title: Oncology letters Volume: 3 Pages: 520-524
  • Peer Reviewed
• [Journal Article] Prevalence of topoisomerase I genetic mutations and UGT1A1 polymorphisms associated with irinotecan in individuals of Asian descent (2011)
  • Author(s): Fukui T, Mitsufuji H, Kubota M, Inaoka H, Hirose M, Iwabuchi K, Masuda N, Kobayashi H
  • Journal Title: Oncology letters Volume: 2 Pages: 923-928
  • Peer Reviewed
• [Journal Article] X-ray radiation causes electromagnetic interference in implantable cardiac pacemakers (2010)
  • Author(s): Hirose M, Tachikawa K, Ozaki M, Umezawa N, Shinbo T, Kokubo K, Kobayashi H
  • Journal Title: Pacing Clin Electrophysiol Volume: 33 Pages: 1174-1181
  • Peer Reviewed
• [Journal Article] Potential utility of HOP homeobox gene promoter methylation as a marker of tumor aggressiveness in gastric cancer (2010)
  • Author(s): Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Kokubo K, KobayashiH, Kim M S, Sidransky D, Watanabe M
  • Journal Title: Oncogene Volume: 29 Pages: 3263-327
  • Peer Reviewed
• [Journal Article] Removal of an endotoxin fragment(Lipid A) by an endotoxin-retentive filter (2010)
  • Author(s): Kokubo K, Futatsugi K, Tsukao H, Shinbo T, Hirose M, Kobayashi H
  • Journal Title: Desalination and Water Treatment Volume: 17 Pages: 25-30
  • Peer Reviewed
• [Journal Article] Macrophages that survive hyperoxia exposure have higher superoxide dismutase activities in their mitochondria (2010)
  • Author(s): Kokubo K, Soeda S, Shinbo T, Hirose M, Fuku N, Nishigaki Y, Tanaka M, Kobayashi H
  • Journal Title: Adv Exp Med Biol Volume: 662 Pages: 63-69
  • Peer Reviewed
• [Journal Article] Respiratory Rhythms in Healthy Subjects and Patients (2010)
  • Author(s): Shimane M, Tomita Y, Shinbo T, Kokubo K, Hirose M, Hataishi R, Kubota M, Masuda N, Kobayashi H
  • Journal Title: Advances in Experimental Medicine & Biology Volume: 662 Pages: 133-40
  • Peer Reviewed
• [Journal Article] Effect of gefitinib on warfarin antithrombotic activity (2009)
  • Author(s): Arai S, Mitsufuji H, Nishii Y, Onda S, Ryuge S, Wada M, Katono K, Iwasaki M, Takakura A, Otani S, Yamamoto M, Yanaihara T, Yokoba M, Kubota M, Katagiri M, Fukui T, Kobayashi H. Yanase N, Hataishi R, Masuda N
  • Journal Title: Int J Clin Oncol Volume: 14 Pages: 332-336
  • Peer Reviewed
• [Journal Article] Clinical score and transcript abundance patterns identify Kawasaki disease patients who may benefit from addition of methylprednisolone (2009)
  • Author(s): Ogata S, Ogihara Y, Nomoto K, Akiyama K, Nakahata Y, Sato K, Minoura K, Kokubo K, Kobayashi H, Ishii M
  • Journal Title: Pediatric Research Volume: 66 Pages: 577-584
  • Peer Reviewed
• [Journal Article] Low frequency oscillometry parameters in COPD patients are less variable during inspiration than during expiration (2009)
  • Author(s): Kubota M, Shirai G, Nakamori T, Kokubo K, Masuda N, Kobayashi H
  • Journal Title: Respir Physiol Neurobiol. Volume: 166 Pages: 73-79
  • Peer Reviewed
• [Presentation] Increased surface coverage area of polyvinylpyrrolidone on polysulfone hemofilter membrane improves biocompatibility by suppressing cell adhesion during continuous hemofiltration (2011)
  • Author(s): Kurihara Y, Kokubo K, Tsukao H, Shinbo T, Hirose M, Kobayashi H
  • Organizer: 16th International Conference on Continuous Renal Replacement Therapies CRRT2001
  • Place of Presentation: San Diego, USA
  • Year and Date: 20110222-25
• [Presentation] Possible applications of hydrogen gas and nitric oxide for the therapy of critical illness. Invited lecture in the Symposium III : Applications of the Medical Gases NO, CO, H2S and H2 in Critical Illness (2011)
  • Author(s): Kobayashi H
  • Organizer: Shock Society 24th annual conference
  • Place of Presentation: Norfolk, USA
  • Year and Date: 2011-06-14
• [Presentation] Urea concentrating ability of artificial renal tubule based on countercurrent multiplier system using electrodialysis, dialysis and filtration (2010)
  • Author(s): Harashima T, Otani Y, Kokubo K, Tsukao H, Shinbo T, Hirose M, Sakai K, Kobayashi H
  • Organizer: World Conference on portable-wearable and miniaturized systems for dialysis and ultrafiltration and 3rd Annual Congress of the International Society for hemodialysis
  • Place of Presentation: Vicenza, Italy
  • Year and Date: 20100930-1002
• [Presentation] Dialysate-based perfusate of low viscosity is suitable for normothermic ex vivo lung perfusion (2010)
  • Author(s): Noda K, Kokubo K, Shinbo T, Hirose M, Kobayashi H
  • Organizer: ERS Annual Congress 2010
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100918-19
• [Presentation] Inhaled hydrogen during mechanical ventilation and hydrogen generated by enterobacterial flora reduce infarct size in the mouse model of myocardial ischemia-reperfusion injury (2010)
  • Author(s): Sato Y, Shinbo T, Hataishi R, Kokubo K, Hirose M, Kobayashi H
  • Organizer: European Respiratory Society Annual Congress 2010
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100918-19
• [Presentation] Hydrogen protects alveolar epithelial cells(A549) against injury induced by reactive oxygen species in vitro (2010)
  • Author(s): Kokubo K, Yamashita K, Shinbo T, Hirose M, Kobayashi H
  • Organizer: European Respiratory Society Annual Congress 2010
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100918-19
• [Presentation] Temporal changes in gene expressions and cytokine productions caused by stretching of normal human pulmonary artery endothelial cells (2010)
  • Author(s): Kobayashi K, Tanaka M, Inaoka H, Nebuya S, Fukuoka Y, Kokubo K, Kobayashi H, Noshiro M
  • Organizer: European Respiratory Society Annual Congress 2010
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100918-19
• [Presentation] Differentiation of side population cells isolated from human amniotic mesenchymal cell into vascular endothelial cells under hypoxic condition (2010)
  • Author(s): Maruyama N, Nishikubo Y, Kokubo K, Shinbo T, Hirose M, Kobayashi M, Sakuragawa N, Kobayashi H
  • Organizer: Tissue Engineering & Regenerative Medicine International Society 2010 Asia Pacific meeting, TERMIS-AP 2010 Annual Conference
  • Place of Presentation: Sydney, Australia
  • Year and Date: 20100915-17
• [Presentation] Differentiation into neural cells from human amniotic mesenchymal cell in serum free medium (2010)
  • Author(s): Nishikubo Y, Maruyama N, Kokubo K, Shinbo T, Hirose M, Kobayashi M, Sakuragawa N, Kobayashi H
  • Organizer: Tissue Engineering & Regenerative Medicine International Society 2010 Asia Pacific meeting, TERMIS-AP 2010 Annual Conference
  • Place of Presentation: Sydney, Australia
  • Year and Date: 20100915-17
• [Presentation] Comparison of the effects of the dilution mode on inflammation and lymphocyte function between predilution and postdilution hemodiafiltration (2010)
  • Author(s): Sakurai K, Saito T, Kokubo K, Asahi D, Yamauchi F, Hosoya H, Maruyama N, Kobayashi H
  • Organizer: 47th ERA-EDTA Congress
  • Place of Presentation: Munich
  • Year and Date: 20100625-28
• [Presentation] Suppression of platelet Activation on the surface of dialysis membrane releasing nitric oxide from the dialysate (2010)
  • Author(s): Urabe S, Kokubo K, Tsukao H, Shinbo T, Hirose M, Kobayashi H
  • Organizer: 47th ERA-EDTA Congress
  • Place of Presentation: Munich
  • Year and Date: 20100625-28
• [Presentation] Activation of platelets in contact with oxidized vitamin E-coated surface (2010)
  • Author(s): Tsukao H, Kokubo K, Nagasato M, Shinbo T, Hirose M, Kobayashi H
  • Organizer: 47th ERA-EDTA Congress
  • Place of Presentation: Munich
  • Year and Date: 20100625-28
• [Presentation] Simultaneous inhalation of nitric oxide and hydrogen reduces infarct size in the mouse model of myocardial ischemia-reperfusion injury (2010)
  • Author(s): Shinbo T, Sato Y, Hataishi R, Kokubo K, Hirose M, Kobayashi H
  • Organizer: 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: Kyoto
  • Year and Date: 20100614-18
• [Presentation] Dialysis membrane releasing nitric oxide from dialysate side increases cyclic guanosine monophosphate in platelets and suppresses the activation of platelets on the surface (2010)
  • Author(s): Kokubo K, Urabe S, Tsukao H, Shinbo T, Hirose M, Kobayashi H
  • Organizer: 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: Kyoto
  • Year and Date: 20100614-18
• [Patent(Industrial Property Rights)] 虚血再潅流障害軽減用治療剤および治療装置 (2010)
  • Inventor(s): 小林弘祐,小久保謙一,新保年弘
  • Industrial Property Rights Holder: 学校法人北里研究所
  • Industrial Property Number: 特許、特願2010-053405,特開2011-184398
  • Filing Date: 2010-03-10