Development of the therapy for the renal diseases using the cells derived from adipose tissue

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Nagoya University
• Principal Investigator: MARUYAMA Shoich 名古屋大学, 大学院・医学系研究科, 准教授 (10362253)
• Co-Investigator(Kenkyū-buntansha): OZAKI Takenori 名古屋大学, 医学部附属病院, 病院助教 (10452195) ; YUZAWA Yukio 藤田保健衛生大学, 医学部, 教授 (00191479) ; MATSUO Seiichi 名古屋大学, 大学院・医学系研究科, 教授 (70190410)
• Project Period (FY): 2009 – 2011
• Keywords: 幹細胞 / 脂肪 / 間葉系細胞 / 免疫抑制 / 異種移植

Research Abstract

Our final goal is to develop a new cellular therapy for the renal disease by using the regenerative and immunosuppressive action of adipose tissue-derived stem/stromal cells(ASC). This study is a basic study for this purpose. We successfully established an in vitro model to evaluate the T cell suppression as well as in vivo model of suppression of anti-xenoantibody production. These systems to assay immunosuppressive actions of LASC will accelerate our future study to clarify the molecular mechanisms of LASC action.

Research Products

• [Journal Article] Increased urethral resistance by periurethral in jection of low serum cultured adipose-derived mesenchymal stromal cells in rats (2011)
  • Author(s): Watanabe T, Maruyama S, Yamamoto T, Kamo I, Yasuda K, Saka Y, Ozaki T, Yuzawa Y, Matsuo S, Gotoh M.
  • Journal Title: Int J Urol Volume: 18(9) Pages: 659-66
  • Peer Reviewed
• [Journal Article] Tunneling nanotube smediate rescue of prematurely senescent endo the lial cells by endo the lial progenitors : exchange of lysosomal pool (2011)
  • Author(s): Yasuda K, Khandare A, Burianovskyy L, Maruyama S, Zhang F, Nasjletti A, Goligorsky MS.
  • Journal Title: Aging(Albany NY) Volume: 3(6) Pages: 597-608
  • Peer Reviewed
• [Journal Article] Adipose-derived stromal cells cultured in a low-serum medium, but not bone marrow-derived stromal cells, impede xenoantibody production (2011)
  • Author(s): Saka Y, Furuhashi K, Katsuno T, Kim H, Ozaki T, Iwasaki K, Haneda M, Sato W, Tsuboi N, Ito Y, Matsuo S, Kobayashi T, Maruyama S.
  • Journal Title: Xenotransplantation Volume: 18(3) Pages: 196-208
  • Peer Reviewed
• [Journal Article] Peritonitis is still an important factor for withdrawal from peritoneal dialysis therapy in the Tokai area of Japan (2011)
  • Author(s): Mizuno M, Ito Y, Tanaka A, Suzuki Y, Hiramatsu H, Watanabe M, Tsuruta Y, Matsuoka T, Ito I, Tamai H, Kasuga H, Shimizu H, Kurata H, Inaguma D, Hiramatsu T, Horie M, Naruse T, Maruyama S, Imai E, Yuzawa Y, Matsuo S.
  • Journal Title: Clin Exp Nephrol Volume: 15(5) Pages: 727-37
  • Peer Reviewed
• [Journal Article] Atrial natriuretic peptide ameliorates peritoneal fibrosis in rat peritonitis model (2011)
  • Author(s): Kato H, Mizuno T, Mizuno M, Sawai A, Suzuki Y, Kinashi H, Nagura F, Maruyama S, Noda Y, Yamada K, Matsuo S, Ito Y.
  • Journal Title: Nephrol Dial Transplant Volume: 27(2) Pages: 526-36
  • Peer Reviewed
• [Journal Article] Analysis of glialcell line-derived neurotrophic factor-inducible zinc finger protein 1expression in human diseased kidney (2011)
  • Author(s): Saito S, Murakumo Y, Tsuzuki T, Dambara A, Kato T, Enomoto A, Asai N, Maruyama S, Matsuo S, Takahashi M.
  • Journal Title: Hum Pathol Volume: 42(6) Pages: 848-58
  • Peer Reviewed
• [Journal Article] Basigin/CD147 promotes renal fibrosis after unilateral ureteral obstruction (2011)
  • Author(s): Kato N, Kosugi T, Sato W, Ishimoto T, Kojima H, Sato Y, Sakamoto K, Maruyama S, Yuzawa Y, Matsuo S, Kadomatsu K.
  • Journal Title: Am J Pathol Volume: 178(2) Pages: 572-9
  • Peer Reviewed
• [Journal Article] Circulatin gprogenitor cell count for cardiovascular risk stratification : a pooled analysis (2010)
  • Author(s): Fadini GP, Maruyama S, Ozaki T, Taguchi A, Meigs J, Dimmeler S, Zeiher AM, deKreutzenberg S, Avogaro A, Nickenig G, Schmidt-Lucke C, Werner N.
  • Journal Title: PLoS One Volume: 5(7) Pages: e11488
  • Peer Reviewed
• [Journal Article] Prognostic value of reduced left ventricular ejection fraction at start of hemodialysis the rapy on cardiovascular and all-cause mortality in end-stage renal disease patients (2010)
  • Author(s): Yamada S, Ishii H, Takahashi H, Aoyama T, Morita Y, Kasuga H, Kimura K, Ito Y, Takahashi R, Toriyama T, Yasuda Y, Hayashi M, Kamiya H, Yuzawa Y, Maruyama S, MatsuoS, Matsubara T, Murohara T.
  • Journal Title: Clin J Am Soc Nephrol Volume: 5(10) Pages: 1793-8
  • Peer Reviewed
• [Journal Article] Effects of oral cilostazol 100mg BID on long-term patency after percutaneous transluminal angioplasty inpatients with femoropopliteal disease undergoing hemodialysis : a retrospective chart review in Japanese patients (2010)
  • Author(s): Ishii H, Kumada Y, Toriyama T, Aoyama T, Takahashi H, Tanaka M, Kamoi D, Kawamura Y, Yamada S, Hayashi M, Yasuda Y, Yuzawa Y, Maruyama S, Matsuo S, Matsubara T, Murohara T.
  • Journal Title: Clin Ther Volume: 32(1) Pages: 24-33
  • Peer Reviewed
• [Journal Article] Successful cross-breeding of cloned pigs expressing endo-beta-galactosidase C and human decay accelerating factor (2009)
  • Author(s): Yazaki S, Iwamoto M, Onishi A, Miwa Y, Suzuki S, Fuchimoto D, Sembon S, Furusawa T, Hashimoto M, Oishi T, Liu D, Nagasaka T, Kuzuya T, Maruyama S, Ogawa H, Kadomatsu K, Uchida K, Nakao A, Kobayashi T.
  • Journal Title: Xenotransplantation Volume: 16(6) Pages: 511-21
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Kurata K, Maruyama S, Kato S, Sato W, Yamamoto J, Ozaki T, Nitta A, Nabeshima T, Morita Y, Mizuno M, Ito Y, Yuzawa Y, Matsuo S.
  • Journal Title: Am J Physiol Renal Physiol Volume: 297(6) Pages: F1510-7
  • Peer Reviewed
• [Journal Article] A murine model of neonatal diabetes mellitus in Glis3-deficient mice (2009)
  • Author(s): Watanabe N, Hiramatsu K, Miyamoto R, Yasuda K, Suzuki N, Oshima N, Kiyonari H, Shiba D, Nishio S, Mochizuki T, Yokoyama T, Maruyama S, Matsuo S, Wakamatsu Y, Hashimoto H.
  • Journal Title: Am J Physiol Renal Physiol Volume: 583(12) Pages: 2108-13
  • Peer Reviewed
• [Journal Article] The growth factor midkine regulates the renin-angiotensin system in mice (2009)
  • Author(s): Hobo A, Yuzawa Y, Kosugi T, Kato N, AsaiN, Sato W, Maruyama S, Ito Y, Kobori H, Ikematsu S, Nishiyama A, Matsuo S, Kadomatsu K.
  • Journal Title: J Clin Invest Volume: 119(6) Pages: 1616-25
  • Peer Reviewed
• [Journal Article] Kidney regeneration through nephron neogenesis in medaka (2009)
  • Author(s): Watanabe N, Kato M, Suzuki N, Inoue C, Fedorova S, Hashimoto H, Maruyama S, Matsuo S, Wakamatsu Y.
  • Journal Title: Dev Growth Differ Volume: 51(2) Pages: 135-43
  • Peer Reviewed
• [Journal Article] Lymphatic vessels develop during tubulointer stitial fibrosis (2009)
  • Author(s): Sakamoto I, Ito Y, Mizuno M, Suzuki Y, Sawai A, Tanaka A, Maruyama S, Takei Y, Yuzawa Y, Matsuo S
  • Journal Title: Kidney Int Volume: 75(8) Pages: 828-38
  • Peer Reviewed
• [Journal Article] Novel culture system of mesenchymal stromal cells from human subcutaneous adipose tissue (2009)
  • Author(s): Iwashima S, Ozaki T, Maruyama S, Saka Y, Kobori M, Omae K, Yamaguchi H, Niimi T, Toriyama K, Kamei Y, Torii S, Murohara T, Yuzawa Y, Kitagawa Y, Matsuo S
  • Journal Title: Stem Cells Dev Volume: 18(4) Pages: 533-43
  • Peer Reviewed
• [Journal Article] Removal of blood group A/Bantigen in organs by ex vivo and in vivo administration of endo-beta-galactosidase(ABase) for ABO-incompatible transplantation (2009)
  • Author(s): Kobayashi T, Liu D, Ogawa H, Miwa Y, Nagasaka T, Maruyama S, Li YT, Onishi A, Iwamoto M, Kuzuya T, Kadomatsu K, Uchida K, Nakao A.
  • Journal Title: Transpl Immunol Volume: 20(3) Pages: 132-8
  • Peer Reviewed
• [Journal Article] Asymp to matic diverticulosis identified by computed tomography is not a risk factor for enteric peritonitis
  • Author(s): Toda S, Ito Y, Mizuno M, Suzuki Y, Ito I, Hiramatsu H, Ozaki T, Tsuboi N, Sato W, Maruyama S, Imai E, Matsuo S.
  • Journal Title: Nephrol Dial Transplant Volume: (in press)
  • Peer Reviewed
• [Journal Article] Functional Consequences of Inhibiting Exocytosis of Weibel-Palade Bodies in Acute Renal Ischemia
  • Author(s): Yasuda K, Vasko R, Hayek P, Ratliff BB, Bicer H, Mares J, Maruyama S, Bertuglia S, Mascagni P, Goligorsky MS.
  • Journal Title: Am J Physiol Renal Physiol Volume: (in press)
  • Peer Reviewed
• [Presentation] Low Serum Cultured Adipose-Derived Mesenchymal Stem Cells, but Not Bone-Marrow Derived Mesenchymal Stem Cells Ameliorate Rat Crescentic Glomerulonephritis by Functional Polarization of Macrophages into Immunoregulatory Phenotype (2011)
  • Author(s): Kazuhiro Furuhashi
  • Organizer: ASN
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 2011-11-10
• [Presentation] Prognostic Value of Geriatric Nutritional Risk Index for Cardiovascular and All-Cause Mortality in End-Stage Renal Disease Patients on Chronic He modialysis (2011)
  • Author(s): Kaoru Yasuda
  • Organizer: ASN
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 2011-11-10
• [Presentation] Association of Adipokines with Cardiovascular Mortality in Patients on Hem dialysis (2011)
  • Author(s): Shoichi Maruyama
  • Organizer: ASN
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 2011-11-10
• [Presentation] The Pivotal Role of Midkine on the Development of Hypertension in Endothelial Dysfunction (2011)
  • Author(s): Yuka Sato
  • Organizer: ASN
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 2011-11-10
• [Presentation] Low Serum Cultured Adipose Derived Stem Cells Ameliorate Zymosan Induced Severe Rat Peritonitis Model (2011)
  • Author(s): Hangsoo Kim
  • Organizer: ASN
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 2011-11-10
• [Presentation] Clinical Impact of the Examination of the Ocular Fundus for CKD Patients without Atherosclerosis Risk Factors (2011)
  • Author(s): Yoshinari Yasuda
  • Organizer: ASN
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 2011-11-10
• [Presentation] The rapeutic Potential of Adipose. Derived Stem Cells for Anti (2010)
  • Author(s): Kazuhiro Furuhashi
  • Organizer: 42rd Annual Meeting of The American Society of Nephrology
  • Place of Presentation: Colorado Convention Center Denver, CO
  • Year and Date: 2010-11-20
• [Presentation] Analysis of GDNF. Inducible Zinc Finger Protein 1 Expression in Human Diseased Kidney (2010)
  • Author(s): Shoji Saito
  • Organizer: 42rd Annual Meeting of The American Society of Nephrology
  • Place of Presentation: Colorado Convention Center Denver, CO
  • Year and Date: 2010-11-20
• [Presentation] Knock. Down of HGFand VEGF Production Reduces the Renoprotective Effects of Adipose (2010)
  • Author(s): Takayuki Katsuno
  • Organizer: 42rd Annual Meeting of The American Society of Nephrology
  • Place of Presentation: Colorado Convention Center Denver, CO
  • Year and Date: 2010-11-19
• [Presentation] Reactive Protein with Cardiovascular and All Cause Mortality in Hemodialysis Patients (2010)
  • Author(s): Shoichi Maruyama
  • Organizer: 42rd Annual Meeting of The American Society of Nephrology
  • Place of Presentation: Colorado Convention Center Denver, CO
  • Year and Date: 2010-11-18
• [Presentation] Therapeutic potential of adipose-derived stem cells for anti-GBM glomerulonephritis (2010)
  • Author(s): Kazuhiro Furuhashi
  • Organizer: 7^<th> International Conference on Innate Immunity
  • Place of Presentation: ギリシャ
  • Year and Date: 2010-07-04
• [Presentation] Adipose-derived stem cells the rapy is effective in the treatment of anti-GBM glomerulonephritis (2010)
  • Author(s): Kazuhiro Furuhashi
  • Organizer: APCN2010
  • Place of Presentation: Convention and Exhibition Center(COEX), Seoul, Korea
  • Year and Date: 2010-06-06
• [Presentation] RENO PROTECTIVE EFFECTS AND MECHANISMS OF HUMAN ADIPOSE TISSUE-DERIVED S TROMALCELLS CULTURED IN LOW SERUM MEDIA ON ACUTE KIDNEY INJURY (2010)
  • Author(s): T KATSUNO
  • Organizer: APCN2010
  • Place of Presentation: Convention and Exhibition Center(COEX), Seoul, Korea
  • Year and Date: 2010-06-06
• [Presentation] Adipose-derived mesenchymal stem cells suppress antibody production against graft antigens (2010)
  • Author(s): Yosuke-Saka
  • Organizer: ISN nexus
  • Place of Presentation: 京都国際会議センター(ICC京都府)
  • Year and Date: 2010-04-17
• [Presentation] Reno protective effects of human adiposetissue-derived stromal cells cultured in low serum mediaon acute kidney injury (2010)
  • Author(s): Takayuki Katsuno
  • Organizer: ISN nexus
  • Place of Presentation: 京都国際会議センター(ICC京都府)
  • Year and Date: 2010-04-17
• [Presentation] Adipose-derivedstem cells the rapy is effective in the treatment of anti-GBM glomerulonephritis (2010)
  • Author(s): Kazuhiro Furuhashi
  • Organizer: ISN nexus
  • Place of Presentation: 京都国際会議センター(ICC京都府)
  • Year and Date: 2010-04-17
• [Presentation] Reduced number of circulating CD34-positivecells is associated with poor cardiovascular outcomes in chronic hemodialysis patients (2010)
  • Author(s): Shoichi Maruyama
  • Organizer: ISN nexus
  • Place of Presentation: 京都国際会議センター(ICC京都府)
  • Year and Date: 2010-04-17
• [Presentation] Renoprotective effect of human adipose tissue-derived stromal cells in acute kidney injury-Comparison between low and high serum cell culture condition (2009)
  • Author(s): Takayuki Katsuno
  • Organizer: 42rd Annual Meeting of The American Society of Nephrology
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 20091027-1101
• [Patent(Industrial Property Rights)] 脂肪組織由来間葉系肝細胞を含有する免疫抑制剤及びその用途 (2012)
  • Inventor(s): 丸山彰一、尾崎武徳、坂洋祐、古橋和拡、坪井直毅
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Number: 特許、2011-535314
  • Filing Date: 2012-02-17