The investigation of the effect of transplantation of neural crest derived cells to murine focal cerebral ischemia model

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Keio University
• Principal Investigator: TOMITA Yutaka 慶應義塾大学, 医学部, 講師(非常勤) (60276251)
• Project Period (FY): 2009 – 2011
• Keywords: 神経堤細胞 / 脳梗塞 / Confocal顕微鏡 / Matlab software

Research Abstract

The aim of the study was to monitor in vivo and noninvasively the fate of neural crest cells transplanted into the ischemic brain of unirradiated mice. In vivo imaging was performed through a closed cranial window following cell transplantation, using laser-scanning confocal fluorescence microscopy. Repeated in vivo imaging revealed that transplanted cells survived within the ischemic and peri-ischemic cortex, migrated significantly towards the lesion, proliferated. This study indicates that confocal fluorescence microscopy is a reliable and unique tool to repeatedly assess with cellular resolution the in vivo dynamic fate of fluorescent cells transplanted into a mouse brain.

Research Products

• [Journal Article] Repeated longitudinal in vivo imaging of neuro-glio-vascular unit at the peripheral boundary of ischemia in mouse cerebral cortex (2012)
  • Author(s): Masamoto K, Tomita Y, Toriumi H, Unekawa M, Takuwa H, Itoh Y, Suzuki N, Kanno I
  • Journal Title: Neuroscience Volume: (in press)
  • Peer Reviewed
• [Journal Article] Suppressive effect of chronic peroral topiramate on potassium-induced cortical spreading depression in rats (2012)
  • Author(s): Unekawa M, Tomita Y, Toriumi H, Suzuki N
  • Journal Title: Cephalalgia Volume: (in press)
  • Peer Reviewed
• [Journal Article] Sustained decrease and remarkable increase in red blood cell velocity in intraparenchymal capillaries associated with potassium-induced cortical spreading depression (2012)
  • Author(s): Unekawa M, Tomita M, Tomita Y, Toriumi H, Suzuki N
  • Journal Title: Microcirculation Volume: 19(2) Pages: 166-174
  • Peer Reviewed
• [Journal Article] Long-term, repeated measurements of mouse cortical microflow at the same region of interest with high spatial resolution (2011)
  • Author(s): Tomita Y, Pinard E, Tran-Dinh A, Schiszler I, Kubis N, Tomita M, Suzuki N, Seylaz J
  • Journal Title: Brain Research Volume: 1372 Pages: 59-69
  • Peer Reviewed
• [Journal Article] Developmental and circulatory profile of the diploic veins (2011)
  • Author(s): Toriumi H, Shimizu T, Shibata M, Unekawa M, Tomita Y, Tomita M, Suzuki N
  • Journal Title: Microvascular Research Volume: 81 Pages: 97-102
  • Peer Reviewed
• [Journal Article] Oscillating neuro-capillary coupling during cortical spreading depression as observed by tracking of FITC-labeled RBCs in single capillaries (2011)
  • Author(s): Tomita M, Tomita Y, Unekawa M, Toriumi H, Suzuki N
  • Journal Title: NeuroImage Volume: 56(3) Pages: 1001-1010
  • Peer Reviewed
• [Journal Article] Capillary remodeling and collateral growth without angiogenesis after unilateral common carotid artery occlusion in mice (2011)
  • Author(s): Guo H, Itoh Y, Toriumi H, Yamada S, Tomita Y, Hoshino H, Suzuki N
  • Journal Title: Microcirculation Volume: 18(3) Pages: 221-227
  • Peer Reviewed
• [Journal Article] RBC velocities in single capillaries of mouse and rat brains are the same, despite 10-fold difference in body size (2010)
  • Author(s): Unekawa M, Tomita M, Tomita Y, Toriumi H, Miyaki K, Suzuki N
  • Journal Title: Brain Res Volume: 1320 Pages: 69-73
  • Peer Reviewed
• [Journal Article] Exogenous nitric oxide increases microflow but decreases RBC attendance in single capillaries in rat cerebral cortex (2009)
  • Author(s): Tomita M, Osada T, Unekawa M, Tomita Y, Toriumi H, Suzuki N
  • Journal Title: Microvasc Rev Commun Volume: 3 Pages: 11-16
  • Peer Reviewed
• [Journal Article] Dually supplied T-junctions in arteriolo-arteriolar anastomosis in mice : key to local hemodynamic homeostasis in normal and ischemic states (2009)
  • Author(s): Toriumi H, Tatarishvili J, Tomita M, Tomita Y, Unekawa M, Suzuki N
  • Journal Title: Stroke Volume: 40 Pages: 3378-3383
  • Peer Reviewed
• [Presentation] K^+による大脳皮質性拡延性抑制誘発時の局所脳血流と軟膜動脈血管口径の変化 (2011)
  • Author(s): 畝川美悠紀, 冨田裕, 鳥海春樹, 正本和人, 伊藤義彰, 菅野巌, 鈴木則宏
  • Organizer: 第23回日本脳循環代謝学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-04
• [Presentation] マウス一側総頸動脈閉塞後に観察された血管新生を伴わない毛細血管の動脈化と側副血行路の発達: Capillary arterialization and collateral growth without angiogenesis after carotid artery occlusion in mice (2011)
  • Author(s): Guo H, Itoh Y, Toriumi H, Yamada S, Tomita Y, Hoshino H, Suzuki N
  • Organizer: 第36回日本脳卒中学会総会
  • Place of Presentation: 京都
  • Year and Date: 2011-07-30
• [Presentation] Effect of injection of artificial RBCs on murine hemorrhagic hypotension model (2011)
  • Author(s): Tomita Y, Unekawa M, Toriumi H, Masamoto K, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Kanno I, Suzuki N
  • Organizer: XIII International Symposium on Blood Substitutes and Oxygen Therapeutics
  • Place of Presentation: Boston
  • Year and Date: 2011-07-29
• [Presentation] Prolonged moderate hypoxia impairs neuro-glia-vascular coupling in the somatosensory cortex accompanied by parenchymal microvessel dilation and astroglia swelling in mice (2011)
  • Author(s): Kanno I, Masamoto K, Takuwa H, Kawaguchi H, Tomita Y, Suzuki N, Obata T
  • Organizer: Brain 11
  • Place of Presentation: Barcelona
  • Year and Date: 2011-05-26
• [Presentation] Cerebral blood flow restoration mechanisms after unilateral common carotid artery occlusion in mice (2011)
  • Author(s): Yamada S, Guo H, Itoh Y, Toriumi H, Tomita Y, Hoshino H, Suzuki N
  • Organizer: Brain 11
  • Place of Presentation: Barcelona
  • Year and Date: 2011-05-26
• [Presentation] Resident endothelial cells surrounding damaged arterial endothelium reendothelialize the lesion without any involvement of foreign progenitor cells (2011)
  • Author(s): Itoh Y, Toriumi H, Yamada S, Tomita Y, Hoshino H, Suzuki N
  • Organizer: Brain 11
  • Place of Presentation: Barcelona
  • Year and Date: 2011-05-26
• [Presentation] Transhemispheric effect of cortical spreading depression : electroencephalogram was suppressed but erythrocyte velocity in intraparenchymal capillaries was not affected in rat (2011)
  • Author(s): Unekawa M, Tomita Y, Toriumi H, Masamoto K, Kanno I, Suzuki N
  • Organizer: Brain 11
  • Place of Presentation: Barcelona
  • Year and Date: 2011-05-26
• [Presentation] The effect of Artificial RBCs injection for hemorrhagic hypotension in mice (2011)
  • Author(s): Tomita Y, Unekawa M, Toriumi H, Masamoto K, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Kanno I, Suzuki N
  • Organizer: Brain 11
  • Place of Presentation: Barcelona
  • Year and Date: 2011-05-26
• [Presentation] Longitudinal imaging of glio-vascular remodeling induced by chronic hypoxia in mouse somatosensory cortex (2011)
  • Author(s): Masamoto K, Tomita Y, Takuwa H, Unekawa M, Toriumi H, Itoh Y, Suzuki N. Kanno I
  • Organizer: Brain 11
  • Place of Presentation: Barcelona
  • Year and Date: 2011-05-24
• [Presentation] Potassium-induced cortical spreading depression suppressed transhemispheric electroencephalogram but did not affect on red blood cell velocity in intraparenchymal capillaries in rats (2011)
  • Author(s): 畝川美悠紀, 冨田裕, 鳥海春樹, 正本和人, 伊藤義彰, 菅野巖, 鈴木則宏
  • Organizer: 第36回日本微小循環学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-02-11
• [Presentation] Neuro-capillary coupling during cortical spreading depression (2010)
  • Author(s): 冨田稔, 冨田裕, 畝川美悠紀, 鳥海春樹, 鈴木則宏
  • Organizer: 第35回日本微小循環学会総会
  • Place of Presentation: 埼玉
  • Year and Date: 20100226-27
• [Presentation] In vivo visualization of mouse cerebral microcirculation during middle cerebral artery occlusion induced by the suture method (2010)
  • Author(s): Tomita Y, Abe T, Unekawa M, Toriumi H, Masamoto K, Kanno I, Suzuki N
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Korea
  • Year and Date: 2010-10-13
• [Presentation] Cilostazol inhibits leukocyte rolling/adhesion onto the cerebral vessel wall after recirculation (2010)
  • Author(s): Itoh Y, Toriumi H, Koizumi K, Yamada S, Guo H, Tomita Y, Hoshino H, Suzuki N
  • Organizer: 7th World Stroke Congress
  • Place of Presentation: Korea
  • Year and Date: 2010-10-13
• [Presentation] Long-term in vivo investigation of mouse cerebral microcirculation after middle cerebral artery ischemia-reperfusion induced by the suture method (2010)
  • Author(s): Tomita Y, Abe T, Unekawa M, Toriumi H, Masamoto K, Kanno I, Suzuki N
  • Organizer: The 9th World Congress for Microcirculation
  • Place of Presentation: Paris
  • Year and Date: 2010-09-28
• [Presentation] Sustained decrease of red blood cell velocity in intraparenchymal capillaries against potassium-induced cortical spreading depression in rats (2010)
  • Author(s): Unekawa M, Tomita M, Tomita Y, Toriumi H, Kanno I, Suzuki N
  • Organizer: The 9th World Congress for Microcirculation
  • Place of Presentation: Paris
  • Year and Date: 2010-09-28
• [Presentation] Significance of arterio-venous shunt in the diploic veins in mice (2010)
  • Author(s): Toriumi H, Shimizu T, Shibata M, Tomita M, Tomita Y, Unekawa M, Kanno I, Suzuki N
  • Organizer: The 9th World Congress for Microcirculation
  • Place of Presentation: Paris
  • Year and Date: 2010-09-28
• [Presentation] Brain microcirculation during cortical spreading depression (2010)
  • Author(s): 冨田裕
  • Organizer: 第35回日本微小循環学会総会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-02-27
• [Presentation] Variation of red blood cell velocities in intraparenchymal capillaries on potassium-induced cortical spreading depression in anesthetized rats (2010)
  • Author(s): 畝川美悠紀, 冨田稔, 冨田裕, 鳥海春樹, 鈴木則宏
  • Organizer: 第35回日本微小循環学会総会
  • Place of Presentation: 埼玉
  • Year and Date: 2010-02-26
• [Presentation] 麻酔下および覚醒時のK^+誘発性皮質性拡延性抑制によるDC電位の変化および片麻痺 (2009)
  • Author(s): 畝川美悠紀, 冨田稔, 冨田裕, 鳥海春樹, 鈴木則宏
  • Organizer: 第37回日本頭痛学会総会
  • Place of Presentation: 宇都宮
  • Year and Date: 20091128-29
• [Presentation] 片頭痛発作時の血流変化に関する検討-硬膜動脈と板間静脈の血管構造とA-Vシャント (2009)
  • Author(s): 鳥海春樹, 冨田稔, 冨田裕, 清水利彦, 畝川美悠紀, 鈴木則宏
  • Organizer: 第37回日本頭痛学会総会
  • Place of Presentation: 宇都宮
  • Year and Date: 20091128-29
• [Presentation] ラットK^+による脳皮質性拡延性抑制伝播の際の赤血球速度、DC電位、脳波、酸素分圧、脳血流の相互関係 (2009)
  • Author(s): 畝川美悠紀, 冨田稔, 冨田裕, 鳥海春樹, 鈴木則宏
  • Organizer: 第21回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20091119-20
• [Presentation] When does angiogenesis accompanied with RBC flow develop in ischemic tissue after permanent MCA occlusion in C57BL/6J mice (2009)
  • Author(s): Tomita M, Tatarishvili J, Toriumi H, Tomita Y, Unekawa M, Hattori H, Suzuki N
  • Organizer: Brain 09
  • Place of Presentation: Chicago
  • Year and Date: 20090702-03
• [Presentation] マウス局所脳虚血モデルにおける長期皮質微小循環の高空間分解能in vivo定量的測定 (2009)
  • Author(s): 冨田裕
  • Organizer: 第46回日本臨床生理学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2009-10-22
• [Presentation] Artificial RBCs Transiently improved microcirculatory derangement produced by MCA occlusion or hemorrhagic hypotension in mice (2009)
  • Author(s): Tomita Y, Toriumi H, Hattori H, Unekawa M, Tomita M, Itoh Y, Hoshino H, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Suzuki N
  • Organizer: Brain 09
  • Place of Presentation: Chicago
  • Year and Date: 2009-07-02
• [Presentation] FITC-Labeled RBC tracking in arteriolo-arteriolar anastomoses in control state and during ischemia after MCA occlusion in mice (2009)
  • Author(s): Toriumi H, Tatarishvili J, Tomita M, Tomita Y, Hattori H, Unekawa M, Suzuki N
  • Organizer: Brain 09
  • Place of Presentation: Chicago
  • Year and Date: 2009-07-02
• [Presentation] A hypothesis : Virchow-Robin space as an integrating cleft of cerebrocortical local arteriolar responses (2009)
  • Author(s): Tomita M, Tomita Y, Unekawa M, Toriumi H, Hattori H, Suzuki N
  • Organizer: The 10^<th> International Symposium on Vasodilatation
  • Place of Presentation: 松島
  • Year and Date: 2009-06-02