2011 Fiscal Year Final Research Report
Identification of target proteins of anti-endothelial cell antibodies in different racial groups with vasculitis by proteomics
| Project/Area Number |
21591273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
KARASAWA Rie 聖マリアンナ医科大学, 医学(系)研究科(研究院), 講師 (50434410)
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| Co-Investigator(Renkei-kenkyūsha) |
YUDOH Kazuo 聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (60272928)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 膠原病 / 医化学 |
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| Research Abstract |
We attempted to identify candidate target proteins for anti-endothelial cell antibodies(AECA) comprehensively by a proteomic approach. Proteomic analyses showed significant different candidate target proteins between Japanese patients and American patients with large vessel vasculitis. More than 85% of Japanese patients with Takayasu arteritis(Tak) were found to have autoantibodies to Prx2 and PPIA, target proteins of AECA, respectively, whereas less than 15% of American patients with Tak were found to have them. Next, candidate target proteins for AECA were detected in American patients with giant cell arteritis(GCA) by two-dimensional electrophoresis-Western blotting. As a result, 9 endothelium-specific proteins and 22 patient-specific proteins were identified in GCA patients. 15 endothelium-specific spots and 4 patient-specific spots were detected in American patients with Tak. This work demonstrated that it is important to assess target proteins of AECA in different racial groups with vasculitis.
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