Exploration of pathogenetic mechanism for hair diseases through dermal fibroblasts

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591427
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hirosaki University
• Principal Investigator: NAKANO Hajime 弘前大学, 大学院・医学研究科, 准教授 (90281922)
• Co-Investigator(Kenkyū-buntansha): MATSUZAKI Yasushi 弘前大学, 医学部附属病院, 講師 (50322946) ; SAWAMUERA Daisuke 弘前大学, 大学院・医学研究科, 教授 (60196334)
• Project Period (FY): 2009 – 2011
• Keywords: ヒロサキヘアレスラット / 乏毛 / 繊維芽細胞 / 細胞周期 / 脱毛疾患

Research Abstract

Primary culture of the dermal fibroblasts derived from Hirosaki hairless rat (HHR-DF) develops multinuclear large fibroblasts after multiple culture passages. These cells have low proliferation ability. Several growth stimuli including calf serum, TGF-beta, and bFGF failed to promote cell proliferation of HHR-DF. Artificial skin defect on the dorsal skin of HHR showed retarded wound healing. These results suggest that HHR-DF has an impaired sensitivity to growth stimuli.

Research Products

• [Journal Article] Buschke-Ollendorff syndrome associated with hypertrophic scar formation : a possible role for LEMD3 mutation (2012)
  • Author(s): Korekawa A, Nakano H, Toyomaki Y, Takiyoshi N, Rokunohe D, Akasaka E, Nakajima K, Sawamura D
  • Journal Title: Br J Dermatol Volume: 166 Pages: 900-903
  • DOI: DOI:10.1111/j.1365-2133.2011.10691.x
  • Peer Reviewed
• [Journal Article] Diffuse and focal palmoplantar keratoderma can be caused by a keratin 6c mutation (2011)
  • Author(s): Akasaka E, Nakano H, Nakano A, Toyomaki Y, Takiyoshi N, Rokunohe D, Nishikawa Y, Korekawa A, Matsuzaki Y, Mitsuhashi Y, Sawamura D
  • Journal Title: Br J Dermatol Volume: 165(6) Pages: 1290-1292
  • DOI: DOI:10.1111/j.1365-2133.2011.10552.x
  • Peer Reviewed
• [Journal Article] Vorner type palmoplantar keratoderma : novel KRT9 mutation associated with knuckle pad-like lesions and recurrent mutation causing digital mutilation (2011)
  • Author(s): Umegaki N, Nakano H, Tamai K, Mitsuhashi Y, Akasaka E, Sawamura D, Katayama I
  • Journal Title: Br J Dermatol Volume: 165(1) Pages: 199-201
  • DOI: DOI:10.1111/j.1365-2133.2011.10317.x
  • Peer Reviewed
• [Journal Article] Leukemia cutis is an early sign of blast transformation that may mimic a common skin disease : sycosis-like eruptions in chronic myelomonocytic leukemia (2010)
  • Author(s): Takeuchi S, Akasaka E, Rokunohe D, Kayo SJ, Matsuzaki Y, Ooshima H, Nakano H, Sawamura D
  • Journal Title: J Dermatol Volume: 37 Pages: 916-918
  • DOI: DOI:10.1111/j.1346-8138.2010.00901.x
  • Peer Reviewed
• [Journal Article] Characterization of Hair Follicles in Hirosaki Hairless Rats with Deletion of Basic Hair Keratin Genes. Enlarged Medulla, Loss of Cuticle and Long Catagen (2009)
  • Author(s): Akita M, Nanashima N, Yamada T, Nakano H, Shimizu T, Fan Y, Tsuchida S
  • Journal Title: Hirosaki Medical Journal Volume: 60 Pages: 1-11
  • URL: http://ci.nii.ac.jp/naid/110007079174
  • Peer Reviewed
• [Presentation] ヘアケラチン遺伝子の欠損がある弘前ヘアレスラット(HHR)の毛構造の変化、毛周期の変化 (2009)
  • Author(s): 秋田美季
  • Organizer: 第108回日本皮膚科学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-04-25