Development of cancer stem cell specific therapy using gannma-delta T cells and dendritic cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591644
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyushu University
• Principal Investigator: TANAKA Fumiaki 九州大学, 大学病院, 講師 (30332836)
• Co-Investigator(Kenkyū-buntansha): SUDO Tomoya 九州大学, 大学病院, 助教 (50309803)
• Project Period (FY): 2009 – 2011
• Keywords: 癌幹細胞 / 細胞周期 / 肝細胞 / 癌特異的免疫療法 / ガンマデルタT細胞

Research Abstract

We reported the presence of cancer stem cells(CSS), showing the characters of self-renew and multi-potential deviation, in gastrointestinal cancer. Toward the cancer therapy, complete control of cancer cells would be obtained targeting cancer stem cells. We reported in this study that CD13 as cancer stem cell marker. Using MH129 cells(C3H derived HCC), we achieved that antitumor effect. A prevention method, such as injection of CSS before inoculation of MH129 cells, antitumor effect was observed.

Research Products

• [Journal Article] STC2 : A Predictive Marker for Lymph Node Metastasis in Esophageal Squamous-Cell Carcinoma (2011)
  • Author(s): Kita Y, Mimori K, Iwatsuki M, Yokobori T, Ieta K, Tanaka F, Ishii H, Okumura H, Natsugoe S, Mori M.
  • Journal Title: Ann Surg Oncol Volume: 18(1) Pages: 261-72
  • DOI: DOI:10.1245/s10434-010-1271-1
• [Journal Article] FOXC2 is a Novel Prognostic Factor in Human Esophageal SquamousCell Carcinoma (2011)
  • Author(s): Nishida N, Mimori K, Yokobori T, Sudo T, Tanaka F, Shibata K, Ishii H, Doki Y, Mori M.'
  • Journal Title: Ann Surg Oncol Volume: 18(2) Pages: 535-42
  • DOI: DOI:10.1245/s10434-010-1274-y
• [Journal Article] MicroRNA miR-125b is a prognostic marker in human colorectal cancer' (2011)
  • Author(s): Nishida N, Yokobori T, Mimori K, Sudo T, Tanaka F, Shibata K, Ishii H, Doki Y, Kuwano H, Mori M.
  • Journal Title: Int J Oncol Volume: 38(5) Pages: 1437-43
  • DOI: doi:10.3892/ijo.2011.969.
• [Journal Article] MicroRNA-125a-5p is an independent prognostic factor in gastric cancer, and inhibits the proliferation of human gastric cancer cells in combination with trastuzumab (2011)
  • Author(s): Nishida N, Mimori K, Fabbri M, Yokobori T, Sudo T, Tanaka F, Shibata K, Ishii H, Doki Y, Mori M.]
  • Journal Title: Clin Cancer Res Volume: 17(9) Pages: 2725-33
  • DOI: doi:10.1158/1078-0432.CCR-10-2132doi:10.1158/1078-0432.CCR-10-2132
• [Journal Article] Kinesin 18A expression clinical relevance to colorectal cancer progression (2011)
  • Author(s): Nagahara M, Nishida N, Iwatsuki M, Ishimaru S, Mimori K, Tanaka F, Nakagawa T, Sato T, Sugihara K, Hoon DS, Mori M.
  • Journal Title: Int J Cancer Volume: 129(11) Pages: 2543-52
  • DOI: doi:10.1002/ijc.25916.
• [Journal Article] Identification of HLA-A* 0201/-A* 2402-restricted CTL epitope-peptides derived from a novel cancer/testis antigen, MCAK, and induction of a specific antitumor immune response (2011)
  • Author(s): Kawamoto M, Tanaka F, Mimori K, Inoue H, Kamohara Y, Mori M.
  • Journal Title: Oncol Rep Volume: 25(2) Pages: 469-76
  • DOI: doi:10.3892/or.2010.1101.
• [Journal Article] TGM2 Is a Novel Marker for Prognosis and Therapeutic Target in Colorectal Cancer (2010)
  • Author(s): Miyoshi N, Ishii H, Mimori K, Tanaka F, Hitora T, Tei M, Sekimoto M, Doki Y, Mori M.
  • Journal Title: Ann Surg Oncol Volume: 17(4) Pages: 967-72
  • DOI: DOI:10.1245/s10434-009-0865-y
• [Journal Article] ATP11A is a novel predictive marker for metachronous metastasis of colorectal cancer (2010)
  • Author(s): Miyoshi N, Ishii H, Mimori K, Tanaka F, Nagai K, Uemura M, Sekimoto M, Doki Y, Mori M.
  • Journal Title: Oncol Rep Volume: 23(2) Pages: 505-10
  • DOI: Doi:10.3892/or_00000662
• [Journal Article] Overexpression of SUGT1 in human colorectal cancer and its clinicopathological significance (2010)
  • Author(s): Iwatsuki M, Mimori K, Sato T, Toh H, Yokobori T, Tanaka F, Ishikawa K, Baba H, Mori M.
  • Journal Title: Int J Oncol. 2010 Mar Volume: 36(3) Pages: 569-75
  • DOI: Doi:10.3892/ijo_00000531
• [Journal Article] Clinicopathological and prognostic significance of PDCD4 and microRNA-21 in human gastric cancer (2010)
  • Author(s): Motoyama K, Inoue H, Mimori K, Tanaka F, Kojima K, Uetake H, Sugihara K, Mori M.
  • Journal Title: Int J Oncol Volume: 36(5) Pages: 1089-95
  • DOI: Doi:10.3892/ijo_00000590
• [Journal Article] Saline replacement after local resection of the breast : a simple technique with good control and improved patient satisfaction (2010)
  • Author(s): Tanaka F, Mimori K, Tahara K, Inoue H, Mori M.
  • Journal Title: Breast Cancer Volume: 17(4) Pages: 261-4
  • DOI: DOI:10.1007/s12282-009-0145-5
• [Journal Article] Clinical Significance of Stanniocalcin 2 as a Prognostic Marker in Gastric Cancer (2010)
  • Author(s): Yokobori T, Mimori K, Ishii H, Iwatsuki M, Tanaka F, Kamohara Y, Ieta K, Kita Y, Doki Y, Kuwano H, Mori M.'
  • Journal Title: Ann Surg Oncol Volume: 17(10) Pages: 2601-7
  • DOI: DOI:10.1245/s10434-010-1086-0
• [Journal Article] CD13 is a therapeutic target in human liver cancer stem cells (2010)
  • Author(s): Haraguchi N, Ishii H, Mimori K, Tanaka F, Ohkuma M, Kim HM, Akita H, Takiuchi D, Hatano H, Nagano H, Barnard GF, Doki Y, Mori M.
  • Journal Title: J Clin Invest Volume: 120(9) Pages: 3326-39
  • DOI: doi:10.1172/JCI42550.
• [Journal Article] Correlated expression of CD47 and SIRPA in bone marrow and in peripheral blood predicts recurrence in breast cancer patients' (2010)
  • Author(s): Nagahara M, Mimori K, Kataoka A, Ishii H, Tanaka F, Nakagawa T, Sato T, Ono S, Sugihara K, Mori M.
  • Journal Title: Clin Cancer Res Volume: 16(18 Pages: 4625-35
  • DOI: doi:10.1158/1078-0432.CCR-10-0349
• [Journal Article] Clinical significance of ASB9 in human colorectal cancer (2010)
  • Author(s): Tokuoka M, Miyoshi N, Hitora T, Mimori K, Tanaka F, Shibata K, Ishii H, Sekimoto M, Doki Y, Mori M.
  • Journal Title: Int J Oncol Volume: 37(5) Pages: 1105-11
  • DOI: Doi:10.3892/ijo_00000762
• [Journal Article] Strong interaction between the effects of alcohol consumption and smoking on oesophageal squamous cell carcinoma among individuals with ADH1B and/or ALDH2 risk alleles (2010)
  • Author(s): Tanaka F, Yamamoto K, Suzuki S, Inoue H, Tsurumaru M, Kajiyama Y, Kato H, Igaki H, Furuta K, Fujita H, Tanaka T, Tanaka Y, Kawashima Y, Natsugoe S, Setoyama T, Tokudome S, Mimori K, Haraguchi N, Ishii H, Mori M.
  • Journal Title: Gut Volume: 59(11) Pages: 1457-64
• [Journal Article] Defined factors induce reprogramming of gastrointestinal cancer cells (2010)
  • Author(s): Miyoshi N, Ishii H, Nagai KI, Hoshino H, Mimori K, Tanaka F, Nagano H, Sekimoto M, Doki Y, Mori M.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107(1) Pages: 40-5
  • DOI: doi:10.1073/pnas.0912407107
• [Journal Article] Clinicopathological significance of stanniocalcin 2 gene expression in colorectal cancer (2009)
  • Author(s): Ieta K, Tanaka F, Yokobori T, Kita Y, Haraguchi N, Mimori K, Kato H, Asao T, Inoue H, Kuwano H, Mori M.
  • Journal Title: Int J Cancer Volume: 125(4) Pages: 926-31
  • DOI: DOI:10.1002/ijc.24453
• [Journal Article] A platinum agent resistance gene, POLB, is a prognostic indicator in colorectal cancer (2009)
  • Author(s): Iwatsuki M, Mimori K, Yokobori T, Tanaka F, Tahara K, Inoue H, Baba H, Mori M.
  • Journal Title: J Surg Oncol Volume: 100(3) Pages: 261-6
  • DOI: DOI:10.1002/jso.21275
• [Journal Article] Overand under-expressed microRNAs in human colorectal cancer (2009)
  • Author(s): Motoyama K, Inoue H, Takatsuno Y, Tanaka F, Mimori K, Uetake H, Sugihara K, Mori M.
  • Journal Title: Int J Oncol Volume: 34(4) Pages: 1069-75
  • DOI: Doi:10.3892/ijo_00000233
• [Journal Article] Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamous cell carcinoma (2009)
  • Author(s): Kita Y, Mimori K, Tanaka F, Matsumoto T, Haraguchi N, Ishikawa K, Matsuzaki S, Fukuyoshi Y, Inoue H, Natsugoe S, Aikou T, Mori M.
  • Journal Title: Eur J Surg Oncol Volume: 35(1) Pages: 52-8
  • DOI: Doi:10.3892/ijo_00000233
• [Presentation] ゲノム解析から選択されたADH1BまたはALDH2遺伝子多型と食道扁平上皮癌罹患:飲酒と喫煙との相互関係 (2011)
  • Author(s): 田中文明、井上裕、石丸神矢、西田尚弘、岩谷岳、主藤朝也、柴田浩平、三森功士、石井秀始、森正樹
  • Organizer: 第66回日本消化器外科学会
  • Place of Presentation: 名古屋
  • Year and Date: 20110700
• [Presentation] 新規癌精巣抗原MCAKからのCTLエピトープの同定とヒト抗腫瘍効果の誘導 (2011)
  • Author(s): 田中文明、川本恵、主藤朝也、柴田浩平、三森功士、森正樹
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Presentation] ゲノム解析から選択されたADH1BまたはALDH2遺伝子多型は、飲酒と喫煙により食道扁平上皮癌罹患に強く相関する (2011)
  • Author(s): 田中文明、井上裕、石丸神矢、西田尚弘、岩谷岳、古後龍之介、主藤朝也、柴田浩平、三森功士、石井秀始、森正樹
  • Organizer: 第111回日本外科学会
  • Place of Presentation: 紙上開催
  • Year and Date: 2011-05-26
• [Presentation] 新規癌特異遺伝子MCAK癌抗原ペプチド同定と癌特異的免疫治療への応用 (2011)
  • Author(s): 田中文明、川本恵、主藤朝也、柴田浩平、三森功士、森正樹
  • Place of Presentation: 和歌山
  • Year and Date: 2011-05-19
• [Presentation] Strong interaction between alcohol drinking and smoking habits on esophageal squamouse cell carcinoma was observed among only individuals having ADH1B and/or ALDH2 variants (2010)
  • Author(s): Tanaka F, Yamamoto K, Suzuki S, Inoue H, Koshi Mimori, Ishii H, Mori M.
  • Organizer: 第69回日本癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] ADH1BまたはALDH2遺伝子多型を有すると、飲酒と喫煙が食道扁平上皮癌罹患により強く相関する (2010)
  • Author(s): 田中文明、山本健、鈴木貞夫、井上裕、主藤朝也、三森功士、柴田浩平、石井秀始、森正樹
  • Organizer: 第64回日本食道学会学術総会
  • Place of Presentation: 久留米
  • Year and Date: 2010-09-01
• [Presentation] 樹状細胞と癌抗原を用いた消化器癌ワクチン治療:癌幹細胞を標的として (2010)
  • Author(s): 田中文明、古後竜之介、石川健二、主藤朝也、三森功士、柴田浩平、森正樹
  • Organizer: 65回日本消化器外科学会総会
  • Place of Presentation: 下関
  • Year and Date: 2010-07-14
• [Presentation] 樹状細胞と癌抗原を用いた消化器癌ワクチン治療:癌幹細胞を標的として (2010)
  • Author(s): 田中文明、古後竜之介、石川健二、主藤朝也、三森功士、柴田浩平、森正樹
  • Organizer: 第65回日本消化器外科学会総会、シンポジウム
  • Place of Presentation: 下関
  • Year and Date: 2010-07-14
• [Presentation] 固形癌に対する樹状細胞を用いた癌特異的免疫治療の現状と今後の工夫 (2010)
  • Author(s): 田中文明、古後龍之介、主藤朝也、三森功士、柴田浩平、森正樹
  • Organizer: 第31回癌免疫外科研究会、シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-21
• [Presentation] 癌幹細胞を標的とした癌治療開発:癌ワクチン治療への応用 (2010)
  • Author(s): 田中文明、石川健二、主藤朝也、三森功士、柴田浩平、森正樹
  • Organizer: 第110回日本外科学会定期学術集会、ワークショップ1
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-08
• [Presentation] 樹状細胞と癌抗原を用いた進行癌に対する癌ワクチン治療.これまでとこれから. (2010)
  • Author(s): 田中文明、石川健二、主藤朝也、三森功士、柴田浩平、森正樹
  • Organizer: 第7回日本免疫治療学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-20
• [Presentation] 樹状細胞と癌抗原を用いた消化器癌ワクチン治療:未来への展望 (2009)
  • Author(s): 田中文明、石川健二、主藤朝也、三森功士、石川浩一、森正樹
  • Organizer: 第64回日本消化器外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-16
• [Presentation] 樹状細胞と癌抗原を用いた消化器癌ワクチン治療:未来への展望 (2009)
  • Author(s): 田中文明、石川健二、主藤朝也、三森功士、石川浩一、森正樹
  • Organizer: 第64回日本消化器外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-16
• [Presentation] 3ステップ・マイクロアレイ法を用いた新規癌抗原遺伝子の同定 (2009)
  • Author(s): 田中文明、横江毅、石川健二、三森功士、石川浩一、森正樹
  • Organizer: 第13回日本がん免疫学会
  • Place of Presentation: 北九州
  • Year and Date: 2009-06-24
• [Presentation] Cell threrapyの新たなアプローチ-3ステップ・マイクロアレイ法を用いた新規癌抗原遺伝子の同定- (2009)
  • Author(s): 田中文明、横江毅、石川健二、三森功士、石川浩一、森正樹
  • Organizer: 第13回日本がん免疫学会
  • Place of Presentation: 小倉
  • Year and Date: 2009-06-24
• [Presentation] 固形癌に対する樹状細胞を用いた癌特異的免疫治療 (2009)
  • Author(s): 田中文明、石川健二、主藤朝也、三森功士、石川浩一、森正樹
  • Organizer: 30回癌免疫外科研究会、シンポジウム
  • Place of Presentation: 久留米
  • Year and Date: 2009-05-21
• [Presentation] 固形癌に対する樹状細胞を用いた癌特異的免疫治療 (2009)
  • Author(s): 田中文明、石川健二、主藤朝也、三森功士、石川浩一、森正樹
  • Organizer: 第30回癌免疫外科研究会
  • Place of Presentation: 久留米
  • Year and Date: 2009-05-21
• [Presentation] 癌肝細胞を標的とした癌治療開発:癌ワクチン治療への応用 (2009)
  • Author(s): 田中文明、石川健二、三森功士、田原光一郎、井上裕、森正樹
  • Organizer: 第109回日本外科学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-04-04