The role of Aquaporins in normal gall bladder epithelium and gall bladder cancer

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591770
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: University of Toyama
• Principal Investigator: TSUKADA Kazuhiro 富山大学, 大学院・医学薬学研究部(医学), 教授 (90171967)
• Co-Investigator(Kenkyū-buntansha): SHIMADA Yutaka 富山大学, 大学院・医学薬学研究部(医学), 准教授 (30216072) ; OKUMURA Tomoyuki 富山大学, 大学院・医学薬学研究部(医学), 助教 (10533523) ; YOSHIOKA Isaku 富山大学, 大学病院, 助教 (30436430)
• Project Period (FY): 2009 – 2011
• Keywords: 胆道外科学

Research Abstract

We examined AQP expression of gall bladder cancer by tissue microarray. In gall bladder cancer, expression of AQP-1 and AQP-5 was observed in 41% and 59% respectively. AQP-5 expression had inverse association with depth of tumor and positive association with gemcitabine sensitivity. Thus, the patients with positive AQP-5 expression had favorable prognosis. SiRNA treatment for AQP-5 positive gall bladder cancer cell line(NOZ-1) inhibited the growth, invasion and mobility of the cells. We identified several miRNAs that can predict Gemcitabine sensitivity of NOZ-1 by microarray analysis. We also identified the target proteins of detected miRNA. Our results suggest that AQP-5 is promising target of gall bladder cancer treatment.

Research Products

• [Journal Article] Prognostic significance of aquaporins inhuman biliary tract carcinoma (2012)
  • Author(s): Sekine S、Shimada Y, Nagata T, Moriyama M, Omura T, Watanabe T, Hori R, Yoshioka I, Okumura T, Sawada S, Fukuoka J, Tsukada K
  • Journal Title: Oncology Reports
  • DOI: doi:10.3892/or.2012.1747
  • Peer Reviewed
• [Journal Article] Involvement of aquaporin-5 in differentiation of human gastric cancerCells (2009)
  • Author(s): Watanabe T, Fujii T, Oya T, Horikawa N, Tabuchi Y, Takahashi Y, Mori M, Takeguchi N, Tsukada K, Sakai H
  • Journal Title: J Physiol Sci Volume: 59 Pages: 113-122
  • Peer Reviewed
• [Journal Article] 手術の王道II-肝・胆・膵・脾の手術胆嚢癌手術-必要最小限手術の選択 (2009)
  • Author(s): 塚田一博嶋田裕, 坂東正
  • Journal Title: 消化器外科 Volume: 31 Pages: 1985-1991
• [Journal Article] 関根慎一消化器癌の診断・治療胆嚢癌診断と外科治療の選択 (2009)
  • Author(s): 塚田一博、.嶋田裕, 長田拓哉, 澤田成朗, 森田誠市, 奥村知之, 吉岡伊作, 松井恒志, 大澤宗士
  • Journal Title: 消化器外科 Volume: 32 Pages: 869-873
• [Presentation] 胆嚢癌におけるAquaporin発現と腫瘍進展について (2011)
  • Author(s): 関根慎一、澤田成朗、森山亮仁、大村哲也、大澤宗士、橋本伊佐也、北條荘三、渡邊智子、吉岡伊作、松井恒志、堀亮太、吉田徹、長田拓哉、魚谷英之、嶋田裕、福岡順也、塚田一博
  • Organizer: 第9回日本消化器外科学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20111020-23
• [Presentation] The effects of gemcitabine in human gallbladder carcinoma cell lines (2011)
  • Author(s): Sekine S、Sawada S. Moriyama M. Omura T. Yoshioka I. Matsui K. Okumura T. Nagata T. Shimada Y. Tsukada K
  • Organizer: Asian Pacific HPBA Congress 2011
  • Place of Presentation: メルボルン
  • Year and Date: 20110927-30
• [Presentation] ヒト胆嚢癌におけるAquaporinの発現解析 (2010)
  • Author(s): 関根慎一、嶋田裕,大澤宗士,北條荘三,奥村知之,澤田成朗,長田拓哉,塚田一博
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 20100900
• [Presentation] 胆嚢疾患におけるAquaporin5の発現について (2010)
  • Author(s): 関根慎一、嶋田裕,森山亮仁,小島博文,大澤宗士,北條荘三,渡邊智子,吉岡伊作,松井恒志,堀亮太,奥村知之,澤田成朗,吉田徹,長田拓哉,魚谷英之,塚田一博
  • Organizer: 第8回日本消化器外科学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-15
• [Presentation] 組織アレイによる消化器癌における網羅的Aauaporin発現解析 (2010)
  • Author(s): 嶋田裕、奥村知之,大澤宗士,関根慎一,森山亮仁,小島博文,北條荘三,山口哲也,渡邊智子,松井恒志,吉岡伊作,澤田成朗,吉田徹,長田拓哉,魚谷英之,塚田一博
  • Organizer: 第96回日本消化器病学会
  • Place of Presentation: 新潟
  • Year and Date: 2010-04-22
• [Presentation] Prognostic significance of Aquapolin(AQP) expression in gastric and colorectal cancer (2010)
  • Author(s): Osawa S、Shimada Y., Sekine S., Sawada S., Kashima S., Fukuoka J., Okumura T., Tsukada K
  • Organizer: American Association of Cancer Research
  • Place of Presentation: Washington
  • Year and Date: 2010-04-19
• [Presentation] ヒト胃癌におけるAquaporin-5の発現及び臨床経過についての検討 (2009)
  • Author(s): 渡邊智子、酒井秀紀,塚田一博,嶋田裕,廣川慎一郎,魚谷英之,長田拓哉,田澤賢一,吉田徹,澤田成朗,森田誠市
  • Organizer: 第109回日本外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2009-04-03
• [Book] Digestive Surgery胆・膵外科手術 (2010)
  • Author(s): 塚田一博、奥村知之,吉岡伊作
  • Total Pages: 48-64
  • Publisher: メディカルビュー社