2011 Fiscal Year Final Research Report
Molecular mechanisms of joint formation by transcription factor SOX11
| Project/Area Number |
21591931
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWANO Hirotaka 東京大学, 医学部附属病院, 講師 (20345218)
KAWAGUCHI Hiroshi 東京大学, 医学部附属病院, 准教授 (40282660)
IKEDA Toshiyuki 東京大学, 医学部附属病院, 特任助教 (80322759)
OOKUMA Tomotake 東京大学, 医学部附属病院, 助教 (90534909)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 関節病学 |
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| Research Abstract |
We analyzed the expression pattern of SOX11 in development of mouse embryo. Whole mount in situ hybridization revealed that Sox11 was expressed in the mesenchymal condensation and pre-mature chondrocytes in E11. 5-12. 5 embryo. Sox11 expression was maintained in the joint locus, and decreased as the joint matured. Next, we searched the transcriptional target genes of Sox11. Several screening revealed that Sox11 widely induced the cartilage matrix genes like Col2a1 and Acan, as well as the joint formation marker genes like GDF5 and Wnt9a. We further identified the responsive elements of Sox11 on these target genes. Meanwhile, Sox11 inhibited the expression of genes which were related with the endochondral ossification and the cartilage degradation. The molecular mechanisms underlying the inhibition were not elucidated yet.
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