2011 Fiscal Year Final Research Report
Neuroprotective effects of CV-159 viewed from energy metabolism and radical scavenge capacity
| Project/Area Number |
21591976
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Oita University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUROKI Chihiro 大分大学, 医学部, 助教 (30392715)
KITANO Takaaki 大分大学, 医学部, 教授 (20211196)
YOKOI Isao 大分大学, 医学部, 教授 (80150366)
KOGA Hironori 大分大学, 医学部, 助教 (50468013)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 病態生理学 / 核磁気共鳴法 / 脳虚血 / 脳保護 / 電子スピン共鳴法 / CV-159 / クレアチンリン酸 / ATP |
|---|
| Research Abstract |
CV-159 is a selective blocker of Ca^<2+>/calmodulin and a free radical scavenger. Neuroprotective effects of CV-159 on energy metabolism of rat brain were investigated by phosphorous nuclear magnetic resonance spectroscopy. CV-159 was added to superfusate(10 nM-10μM, in vitro application), or was administered intraperitoneally(120μg/kg) to male Wistar rats 1 hr before decapitation(in vivo application). The recovery level of phosphocreatine, energetic buffer of ATP in living cells, 1 h after the reperfusion was significantly higher in intraperitoneally CV-159-administered rats than in the control. But with in vitro application, no neuroprotective effect was observed when brain slices were superfused with ACSF containing CV-159.
|
