2011 Fiscal Year Final Research Report

Elucidation of intracellular signaling on cell death and reversible perioperative platelet dysfunction in cardiopulmonary bypass surgery.

Research Project

Project/Area Number	21592021
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Anesthesiology/Resuscitation studies
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	KAGEYAMA Kyoko 京都府立医科大学, 医学研究科, 助教 (80347468)
Co-Investigator(Kenkyū-buntansha)	NAKAJIMA Yasufumi 京都府立医科大学, 医学研究科, 助教 (70326239) MIZOBE Toshiki 京都府立医科大学, 医学研究科, 講師 (50239266)
Project Period (FY)	2009 – 2011
Keywords	遺伝子治療 / 人工心肺 / 周術期
Research Abstract	In the perioperative period of open-heart surgery under cardiopulmonary bypass, hemostasis may be difficult due to decreased platelet function. This study was conducted to study changes over time of intracellular signal transduction system and its role with a focus on p38MAPK pathway in platelet cells. As a result, we observed decreased expression of thrombin receptor, such as GPIb and PAR-1 after withdrawal from cardiopulmonary bypass. Also, we obserbed elevated levels of Bax in the platelet and the decreased levels of cytochrome C in mitochondria, the migration of Bax to mitochondria has been associated with decreased platelet function has been suggested. We thought to maintain the function of platelets, it is important to control of Bax signaling in the platelet.