Novel oncolytic HSV-1 for treatment of slowly-growing tumors such as prostate carcinoma or renal cell carcinoma

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: The University of Tokyo
• Principal Investigator: FUKUHARA Hiroshi 東京大学, 医学部附属病院, 講師 (20292948)
• Co-Investigator(Kenkyū-buntansha): NOMIYA Akira 東京大学, 医学部附属病院, 助教 (30372379)
• Project Period (FY): 2009 – 2011
• Keywords: 前立腺癌 / 腎癌 / ウイルス療法 / 遺伝子治療

Research Abstract

We constructed novel oncolytic HSV-1 using G47Δ's structure, with mutations in the ICP6, g34. 5, and a47 genes, as the basic backbone. The new virus, T-hTERT, contains a functioning ICP6 gene(encoding the large subunit of viral RR) controlled by tumor specific promoter. The in vitro replication capability of T-hTERT was higher than the control virus T-01, with an ICP6 deletion, in some and comparable to T-01 in other cell lines tested. In athymic mice bearing subcutaneous tumors, intraneoplastic injections with T-hTERT demonstrated higher efficacy than T-01 for renal cell carcinoma or prostate cancer.

Research Products

• [Journal Article] Leukopenia as arisk factor for osteonecrosis of the jaw in metastatic prostate cancer receiving zoledronic acid and docetaxel (2012)
  • Author(s): Miyazaki H, Kume H, Suzuki M, Fujimura T, Fukuhara H, Enomoto Y, Nishimatsu H, Homma Y
  • Journal Title: BJU Int Volume: (in press)
• [Journal Article] Association between lower urinary tract symptoms and sexual dysfunction assessed using the core lower urinary tract symptom score and International index of erectile function-5 questionnaires (2012)
  • Author(s): Nakamura M, Fujimura T, Nagata M, Hosoda C, Suzuki M, Fukuhara H, Enomoto Y, Nishimatsu H, Kume H, Igawa Y, Homma Y
  • Journal Title: Aging Male Volume: (in press)
• [Journal Article] Management trends, angioembolization performance and multiorgan injury indicators of renal trauma from Japanese administrative claims database (2012)
  • Author(s): Sugihara T, Yasunaga H, Horiguchi H, Nishimatsu H, Fukuhara H, Enomoto Y, Kume H, Ohe K, Matsuda S, Homma Y
  • Journal Title: Int J Urol Volume: (in press)
• [Journal Article] Assessment of lower urinary tract symptoms in men by international prostate symptom score and core lower urinary tract symptom score (2011)
  • Author(s): Fujimura T, Kume H, Nishimatsu H, Sugihara T, Nomiya A, Tsurumaki Y, Miyazaki H, Suzuki M, Fukuhara H, Enomoto Y, Homma Y
  • Journal Title: BJU Int Volume: (in press)
• [Journal Article] 17β-Estradiol-Mediated Elevation of Peripheral White Blood Cell Count During Estramustine Phosphate Therapy for Prostate Cancer (2011)
  • Author(s): Suzuki M, Fujimura T, Fukuhara H, Enomoto Y, Nishimatsu H, Ishikawa A, Kume H, Homma Y, Kitamura T
  • Journal Title: Int J Endocrinol Metab Volume: 9 Pages: 347-351
• [Journal Article] Core lower urinary tract symptom score(CLSS) for the assessment of female lower urinary tract symptoms : acomparative study (2011)
  • Author(s): Fujimura T, Kume H, Tsurumaki Y, Yoshimura Y, Hosoda C, Suzuki M, Fukuhara H, Enomoto Y, Nishimatsu H, Homma Y
  • Journal Title: Int J Urol Volume: 18 Pages: 778-84
• [Journal Article] Association of rs6983561 Polymorphism at 8q24 With Prostate Cancer Mortality in a Japanese Population (2011)
  • Author(s): Suzuki M, Liu M, Kurosaki T, Suzuki M, Arai T, Sawabe M, Kasuya Y, Kato M, Fujimura T, Fukuhara H, Enomoto Y, Nishimatsu H, Ishikawa A, Kume H, Homma Y, Kitamura T
  • Journal Title: Clin Genitourin Cancer Volume: 9 Pages: 46-52
• [Journal Article] Aberrations of a cell adhesion molecule CADM4 in renal clear cell carcinoma (2011)
  • Author(s): Nagata M, Sakurai-Yageta M, Yamada D, Goto A, Ito A, Fukuhara H, Kume H, Morikawa T, Fukayama M, Homma Y, Murakami Y
  • Journal Title: Int J Cancer Volume: 130 Pages: 1329-1337
• [Journal Article] Docetaxel as a vital option for corticosteroid-refractory prostate cancer (2011)
  • Author(s): Kume H, Suzuki M, Fujimura T, FukuharaH, Enomoto Y, Nishimatsu H, Ishikawa A, Homma Y
  • Journal Title: Int Urol Nephrol Volume: 185 Pages: 1611-1614
• [Journal Article] Prognostic Factors for Renal Cell Carcinoma With Bone Metastasis : Who Are the Long-Term Survivors? (2011)
  • Author(s): Kume H, Kakutani S, Yamada Y, Shinohara M, Tominaga T, Suzuki M, Fujimura T, Fukuhara H, Enomoto Y, Nishimatsu H, Homma Y
  • Journal Title: J Urol Volume: 185 Pages: 1611-1614
• [Journal Article] Lower incidence of inguinal hernia after radical prostatectomy using open gasless endoscopic single-site surgery (2011)
  • Author(s): Fukuhara H, Nishimatsu H, Suzuki M, Fujimura T, Enomoto Y, Ishikawa A, Kume H, Homma Y
  • Journal Title: Prostate Cancer Prostatic Dis Volume: 14 Pages: 162-167
• [Journal Article] Maximum tumor diameter : a simple independent predictor for biochemical recurrence after radical prostatectomy (2010)
  • Author(s): Fukuhara H, Kume H, Suzuki M, Fujimura T, Enomoto Y, Nishimatsu H, Ishikawa A, Homma Y
  • Journal Title: Prostate Cancer Prostatic Dis Volume: 13 Pages: 244-247
• [Journal Article] Distant metastasis of renal cell carcinoma with a diameter of 3 cm or less-which is aggressive cancer? (2010)
  • Author(s): Kume H, Suzuki M, Fujimura T, Fukuhara H, Enomoto Y, Nishimatsu H, Homma Y
  • Journal Title: J Urol Volume: 184 Pages: 64-68
• [Journal Article] Final report on low-dose estramustine phosphate(EMP) monotherapy and very low-dose EMP therapy combined with LH-RH agonist for previously untreated advanced prostate cancer (2010)
  • Author(s): Kitamura T, Suzuki M, Nishimatsu H, Kurosaki T, Enomoto Y, Fukuhara H, Kume H, Takeuchi T, Miao L, Jiangang H, Xiaoqiang L
  • Journal Title: Aktuelle Urol Volume: 41 Pages: S34-40
• [Journal Article] Identification of DBC1 as a transcriptional repressor for BRCA1 (2010)
  • Author(s): Hiraike H, Wada-Hiraike O, Nakagawa S, Koyama S, Miyamoto Y, Sone K, Tanikawa M, Tsuruga T, Nagasaka K, Matsumoto Y, Oda K, Shoji K, Fukuhara H, Saji S, Nakagawa K, Kato S, Yano T, Taketani Y
  • Journal Title: Br JCancer Volume: 102 Pages: 1061-1064
• [Journal Article] Repression of estrogen receptor beta function by putative tumor suppressor DBC1 (2010)
  • Author(s): Koyama S, Wada-Hiraike O, NakagawaS, Tanikawa M, Hiraike H, Miyamoto Y, Sone K, Oda K, Fukuhara H, Nakagawa K, Kato S, Yano T, Taketani Y
  • Journal Title: Biochem Biophys Res Commun Volume: 392 Pages: 357-362
• [Journal Article] Oncolytic virus therapy for prostate cancer (2010)
  • Author(s): Fukuhara H, Homma Y and Todo T
  • Journal Title: Int J Urol Volume: 17 Pages: 20-30