Bio-medical function of GPR91 in renal and urinary tumors

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: KAWAKAMI Takahiro 滋賀医科大学, 医学部, 非常勤講師 (90346023)
• Co-Investigator(Renkei-kenkyūsha): CYANO Tokuhiro 滋賀医科大学, 医学部, 准教授 (40346028)
• Project Period (FY): 2009 – 2011
• Keywords: 腎細胞癌 / 精巣腫瘍 / エピジェネティクス / 胎児性癌 / 遺伝子治療

Research Abstract

We have analyzed various epigenetic profiles in renal and urinary tumors, and identified that the underlying mechanisms to undergo or maintain demethylation of DNA repetitive sequences(LINE1 and Alu repeats) differ between testicular germ cell tumors(TGCTs) and cancer cells of somatic tissue origin. These methylation patterns most likely reflect the origin of TGCTs. Further elucidation of the mechanisms behind the maintenance of DNA hypomethylation in TGCTs is necessary in order to clarify the basic biology of this unique neoplasm.
Otherwise, we have established the potential of SOX2 silencing therapy for embryonal carcinomas, although further improvements are needed in SOX2-siRNA delivery to the tumor.

Research Products

• [Journal Article] Potential of SOX2 Inhibition for Embryonal Carcinoma (2012)
  • Author(s): Ushida H, Chano T, Minami K, Kita H, Kawakami T, Okabe H, Okada Y, Okamoto K.
  • Journal Title: J Urol Volume: 187 Pages: 1876-1881
  • DOI: DOI:10.1016/j.juro.2011.12.058
  • Peer Reviewed
• [Journal Article] Methylation profile of DNA repetitive elements in human testicular germ cell tumor (2011)
  • Author(s): Ushida H, Kawakami T, Minami K, Chano T, Okabe H, Okada Y, Okamoto K.
  • Journal Title: Mol Carcinog
  • DOI: DOI:10.1002/mc.20831
  • Peer Reviewed
• [Journal Article] DMNT3L is a novel marker and is essential for the growth of human embryonal carcinoma (2010)
  • Author(s): Minami K, Chano T, Kawakami T, Ushida H, Kushima R, Okabe H, Okada Y, Okamoto K.
  • Journal Title: Clin Cancer Res Volume: 16 Pages: 2751-2759
  • DOI: DOI:10.1158/1078-0432.CCR-09-3338
  • Peer Reviewed
• [Presentation] DMNT3L is a novel marker and is essential for the growth of human embryonal carcinoma (2010)
  • Author(s): Minami K, Chano T, Kawakami T, Ushida H, Kushima R, Okabe H, Okada Y, Okamoto K.
  • Organizer: 平成22年度日本癌学会総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 20100922-24
• [Presentation] DNMT3L is a novel marker for human embryonal carcinoma of the testis (2009)
  • Author(s): Minami K, Chano T, Kawakami T, Ushida H, Kushima R, Okabe H, Okada Y, Okamoto K.
  • Organizer: 平成21年度分子生物学学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 20091209-12
• [Presentation] DNMT3L is a novel marker for human embryonal carcinoma of the testis (2009)
  • Author(s): Minami K, Chano T, Kawakami T, Ushida H, Kushima R, Okabe H, Okada Y, Okamoto K.
  • Organizer: 平成21年度日本癌学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 20091001-03