2011 Fiscal Year Final Research Report
The research on the usefulness of stem cells derrived adipose tissue for reconering renal funcion after partail nephrectomy
| Project/Area Number |
21592071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Nagoya University |
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Principal Investigator |
SASSA Naoto 名古屋大学, 医学部附属病院, 助教 (50437026)
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| Co-Investigator(Kenkyū-buntansha) |
HATTORI Ryohei 名古屋大学, 医学系研究科, 准教授 (20324410)
YAMAMOTO Tokunori 名古屋大学, 医学部附属病院, 講師 (20182636)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 脂肪由来幹細胞 / 腎部分切除術 / 尿細管周囲毛細血管血流 / 時空間画像処理 |
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| Research Abstract |
Background.
Acute renal ischemic injury(AKI) represents a major clinical problem with renal arterial clamp at parial nephrectony. The use of therapy using adipose tissue-derived stem cells(ASCs) has been suggested as a potential modality to attenutate the ischemic renal damage. Methods. We investigated the possible renoprotection of pretreatment of ASCs before and after in a rat ischemia. reperfusion(I. R) model of AKI. Twenty-four hours postischemia, blood flow in peritubular capillaries(PTC) was measured using intravital videomicroscopy.
Results.
We demonstrated that ADRC therapy significantly reduced serum creatinine and BUN. Histological analysis further validated a significantly attenuated tubular damage. Intravital videomicroscopy and measurement of red blood cell velocity in peritubular capillaries showed ASCs-injected kidneys displayed significant hemodynamic improvement.
Conclusions.
The subscapular administration of ASCs to the kidney attenuates I/R renal injury though antiinflammation, antiapotoyic effect and peritubular capillary microcirculation. The present study suggests that ASCs would be a useful tool in preventing ishemic kidney damage in the clinical setting.
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[Journal Article] Low serum cultured adipose tissue-derived stromal cells ameliorate acute kidney injury in rats2012
Author(s)
Katsuno T, Ozaki T, Furuhashi K, Kim H, Yasuda K, Yamamoto T, Sato W, Tsuboi N, Mizuno M, Ito Y, Imai E, Matsuo S, Matuyama S
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