Extracellular matrix as a new therapeutic target for choroidal neovascularization

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592237
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nagoya City University
• Principal Investigator: NOZAKI Miho 名古屋市立大学, 大学院・医学研究科, 講師 (00295601)
• Co-Investigator(Kenkyū-buntansha): OGURA Yuichiro 名古屋市立大学, 大学院・医学研究科, 教授 (70191963) ; YASUKAWA Tsutomu 名古屋市立大学, 大学院・医学研究科, 准教授 (00324632)
• Project Period (FY): 2009 – 2011
• Keywords: 加齢黄斑変性 / 脈絡膜新生血管 / VEGF-A / オステオポンチン / マクロファージ

Research Abstract

Osteopontin(OPN) is a chemokine like phospholyrated glycoprotein and involved in inflammation, tumor genesis and wound healing. The purpose of the study was to clarify a role of OPN in the development of laser-induced choroidal neovascularization(CNV) in mice. OPN neutralizing Abs significantly abolished CNV area. Genetic ablation of OPN also significantly suppressed CNV area compared with wild-type mice. OPN was immunopositive in the CNV lesions and co-localized with infiltrated macrophages. Collectively these findings demonstrate a significant role of OPN in the development of CNV. OPN blockade may be considered further therapeutic potential for age-related macular degeneration.

Research Products

• [Journal Article] Non-targeted siRNA suppresses laser-induced choroidal neovascularization (2010)
  • Author(s): Shikari M, Tokoro M, Itaya M, Nozaki M, Ogura Y
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51巻 Pages: 3820-3824
  • Peer Reviewed
• [Journal Article] CCR3 is a target for age-related macular degeneration diagnosis and therapy (2009)
  • Author(s): Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J
  • Journal Title: Nature Volume: 460巻 Pages: 225-230
  • Peer Reviewed
• [Presentation] Anti-angiogenic effct of IFN-gamma and IL-12 on laser-induced choroidal neovascularizationmodel in mice (2011)
  • Author(s): Masayuki Ashikari, Miho Nozaki, Takeshi Mizutani, Yuichiro Ogura
  • Organizer: ARVO 2011
  • Place of Presentation: フォートローダーデール(アメリカ)
  • Year and Date: 20110501-05
• [Presentation] Anti-angiogenic effect of fidarestat on laser-induced choroidal neovascularizationin mice (2011)
  • Author(s): Miho Nozaki, Atsushi Nakajima, Nobutaka Morimoto, Noriaki Kato, Yuichiro Ogura
  • Organizer: ARVO 2011
  • Place of Presentation: フォートローダーデール(アメリカ)
  • Year and Date: 20110501-05
• [Presentation] CCR3 antagonist suppresses laser-induced choroidal neovascularization in mice (2010)
  • Author(s): Masayuki Ashikari, Mayumi Tokoro, Miho Nozaki, Yuichiro Ogura
  • Organizer: Euretina
  • Place of Presentation: パリ(フランス)
  • Year and Date: 20100900