Gene transduction of VEGF(vascular endothelial growth factor) receptor2 for intestine ischemia reperfusion induced lung injury.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592311
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Yokohama City University
• Principal Investigator: BABA Yasuko 横浜市立大学, 市民総合医療センター手術部, 講師 (80453041)
• Co-Investigator(Kenkyū-buntansha): KURAHASHI Kiyoyasu 横浜市立大学, 附属市民総合医療センター麻酔科, 准教授 (50234539) ; YAZAWA Takuya 杏林大学, 医学部, 准教授 (50251054)
• Co-Investigator(Renkei-kenkyūsha): KANEGAE Yumi 東京大学, 医科学研究所, 助手 (80251453)
• Project Period (FY): 2009 – 2011
• Keywords: 集中治療学

Research Abstract

The vascular endothelial growth factor(VEGF) plays a role for progressing permeability of lung vessels. We constructed the recombinant adenovirus that expressing the extracellular domain of VEGF receptor II(Ad-VEGFR2), and tried to suppress the effect of the VEGF. The model of lung injury, we used the ICR male mice, clamped the supra mesenteric artery for 30 min's and after that, re-perfused the intestines.
We investigated the effect of VEGF for lung permeability or inflammation, but couldn' t get the enough results than we have expected.