Inhibition of P. gingivalis infection by the SLPI from gingival epithelial cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592340
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
• Co-Investigator(Kenkyū-buntansha): NEMOTO Yuko (NEMOTO OHARA Yuko) 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (10164667)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133) ; SHIMOYAMA Yu 岩手医科大学, 歯学部, 助教 (90453331)
• Project Period (FY): 2009 – 2011
• Keywords: SLPI / 歯肉上皮細胞 / P. gingivalis / 感染抑制

Research Abstract

The results indicated that gingival epithelial cells could be a substantial producer of secretory leukocyte protease inhibitor(SLPI) that functions inhibitory to the pathogenic Porphyromonas gingivalis protease in gingival crevices. It was also suggested that the SLPI production could increase in response to P. gingivalis through the stimulation with its pathogenic constituents.

Research Products

• [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper(2+) ions (2011)
  • Author(s): Taira, M., Shimoyama, Y., Kagiya, T., Sasaki, M., Nezu, T., Harada, H. and Kimura, S.
  • Journal Title: Dent. Mater. J. Volume: 30 Pages: 293-299
• [Journal Article] Asp-and Glu-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalis that ensures utilization of proteineous energy sources (2011)
  • Author(s): Ohara-Nemoto, Y., Shimoyama, Y., Kimura, S., Kon, A., Haraga, H., Ono, T. and Nemoto, T. K.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 38115-38127
• [Journal Article] Quantitative real-time RT-PCR analyses of DNA-damage-recovery-related gene expressions of mouse macrophage-like cell line RAW264 when exposed to IC50 nickel ions (2011)
  • Author(s): Taira, M., Kagiya, T., Sasaki, M. and Kimura, S.
  • Journal Title: Nano Biomed. Volume: 3 Pages: 294-299
• [Journal Article] Microscopic observations and inflammatory cytokine productions of human macrophage phagocytising submicron titanium particles (2010)
  • Author(s): Taira, M., Kagiya, T., Harada, H., Sasaki, M., Kimura, S., Narushima T., Nezu, T. and Araki, Y.
  • Journal Title: J. Mater. Sci : Mater. Med. Volume: 21 Pages: 267-275
• [Journal Article] Amino acid residues modulating the activities of staphylococcal glutamyl endopeptidases (2010)
  • Author(s): Ono, T., Ohara-Nemoto, Y., Shimoyama, Y., Okawara, H., Kobayakawa, T., Baba, T. T., Kimura, S. and Nemoto, T. K.
  • Journal Title: Biol. Chem. Volume: 391 Pages: 1221-1232
• [Journal Article] Relationship between oral status and prevalence of periodontopathic bacteria on the tongues of elderly individuals (2010)
  • Author(s): Kishi, M., Ohara-Nemoto, Y., Takahashi, M., Kishi, K., Kimura, S. and Yonemitsu, M.
  • Journal Title: J. Med. Microbiol. Volume: 59 Pages: 1354-1359
• [Journal Article] Histological and TEM observation of subcutaneous tissues exposed to particulate copper, nickel and titanium (2010)
  • Author(s): Saitoh, S., Sasaki, K., Nezu, T., Taira, M., Shimoyama, Y., Sasaki, M., Kimura, S. and Ishizeki, K.
  • Journal Title: J. Oral Tissue Engin. Volume: 8 Pages: 102-106
• [Journal Article] Determination of three amino acids that caused the alteration of proteolytic activities of staphylococcal glutamyl endopeptidases (2009)
  • Author(s): Nemoto, T. K., Ono, T., Shimoyama, Y., Kimura, S. and Ohara-Nemoto, Y.
  • Journal Title: Biol. Chem. Volume: 390 Pages: 277-285
• [Journal Article] Relationship of quantitative salivary levels of Streptococcus mutans and S. sobrinus in mothers to caries status and colonization of mutans streptococci in plaque in their 2.5-year-old children (2009)
  • Author(s): Kishi, M., Abe, A., Kishi, K., Ohara-Nemoto, Y., Kimura, S. and Yonemitsu, M.
  • Journal Title: Community Dent. Oral Epidemiol. Volume: 37 Pages: 241-249
• [Journal Article] Effects of fosfomycin on Shiga toxin-producing Escherichia coli : quantification of copy numbers of Shiga toxin genes and their expression levels using real-time PCR (2009)
  • Author(s): Ichinohe, N., Ohara-Nemoto, Y., Nemoto, T. K., Kimura, S. and Ichinohe, S.
  • Journal Title: J. Med. Microbiol. Volume: 58(Pt 7) Pages: 971-973
• [Journal Article] Gene expression analyses of human macrophage phagocytizing sub-μtitanium particles by allergy DNA chip(Genopal^<TM>) (2009)
  • Author(s): Taira, M., Nezu, T., Sasaki, M., Kimura, S., Kagiya, T., Harada, H., Narushima T. and Araki, Y.
  • Journal Title: Biomed. Mater. Eng Volume: 19 Pages: 63-70
• [Journal Article] DNA microarray analyses of the effects of LPS-stimulation and IC50 nickel ions on gene expressions of mouse macrophage-like cell line RAW264 (2009)
  • Author(s): Taira, M., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Kimura, S. and Araki, Y.
  • Journal Title: Nano Biomed. Volume: 1 Pages: 59-69
• [Journal Article] First-stage genome-wide gene expression analyses of human mesenchymal stem cells exposed to IC50 Ni(2+) ions. (2009)
  • Author(s): Taira, M., Kagiya, T., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Harada, H., Kimura, S. and Araki, Y.
  • Journal Title: J. Oral Tissue Engin. Volume: 7 Pages: 107-120
• [Journal Article] Characterization of aerosols and fine particles produced in dentistry and their health risk assessments (2009)
  • Author(s): Taira, M., Sasaki, M., Kimura, S. and Araki, Y.
  • Journal Title: Nano Biomed. Volume: 1 Pages: 9-15
• [Presentation] Porphyromonas gingivalis DPP11の酵素活性とAsp/Glu特異性を規定するArg670 (2011)
  • Author(s): 根本孝幸, 小野俊雄, 下山佑, 木村重信, 根本優子
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜
  • Year and Date: 20111000
• [Presentation] Porphyromonas属菌が産生するAsp/Glu特異的新規ジペプチジルペプチダーゼ(DPP) 11 (2011)
  • Author(s): 根本優子, 下山佑, 木村重信, 根本孝幸
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜
  • Year and Date: 20111000
• [Presentation] The pathogenic involvement of the fibronectin binding protein, Fbp62, in Streptococcus anginosus infection. (2011)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Agato, S. and Kimura, S.
  • Organizer: International Union of Microbiological Societies 2011 Congress(IUMS 2011)
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20110900
• [Presentation] Fbp62, fibronectin binding protein of Streptococcus anginosus involves the virulence in mouse infection (2011)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S.
  • Organizer: 10th World Congress of Inflammation
  • Place of Presentation: Paris, France
  • Year and Date: 20110600
• [Presentation] Auto-catalytic propeptide processing facilitates the final hetero-catalytic maturation of glutamyl endopeptidase GluV8 (2011)
  • Author(s): Ohara-Nemoto, Y., Rouf, S. M. A., Shimoyama, Y., Kimura, S., Ono, T. and Nemoto, T. K.
  • Organizer: 111th General Meeting of the American Society for Microbiology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20110500
• [Presentation] Fibronectin binding activity of Streptococcus anginosus promotes the adherence to mucosal epithelial cells (2011)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S.
  • Organizer: 4th International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai, Japan
  • Year and Date: 20110300
• [Presentation] Structural chemistry of Staphylococcus aureus glutamyl endopeptidase (2010)
  • Author(s): Ohara-Nemoto, Y., Rouf, S. M. A., Shimoyama, Y., Kimura, S. and Nemoto, T. K.
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100700
• [Presentation] SLPI production from gingival epithelial cells against Porphyromonas gingivalis infection (2010)
  • Author(s): Ishikawa, T., Ohara-Nemoto, Y., Shimoyama, Y., Sasaki, M. and Kimura, S.
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100700
• [Presentation] Maternal transmission of mutans and other oral streptococcal species (2010)
  • Author(s): Otake-Asakawa, A., Kimura, S., Asakawa, T. and Tanaka, M.
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100700
• [Presentation] Prediction of colonization of periodontopathogens in plaque by VSC measurement (2010)
  • Author(s): Kishi, M., Ohara-Nemoto, Y., Kimura, S., Aizawa, F., Kishi, K., Takahashi, M. and Yonemitsu, M.
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100700
• [Presentation] Adhesion mechanism of Streptococcus anginosus to mucosal epithelial cells (2010)
  • Author(s): Kodama, Y., Sasaki, M., Shimoyama, Y., Tajika, S. and Kimura, S.
  • Organizer: 20th European Congress of Clinical Microbiology and Infectious Diseases
  • Place of Presentation: Vienna, Austria
  • Year and Date: 20100400
• [Presentation] Three amino acid residues define the proteolytic activities of Staphylococcal Glu-specific proteases (2009)
  • Author(s): Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T. K.
  • Organizer: 109th General Meeting of the American Society for Microbiology
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 20090500
• [Book] The production of secretory leukocyte protease inhibitor from gingival epithelial cells in response to Porphyromonas gingivalis lipopolysaccharides. (2010)
  • Author(s): Ishikawa, T., Ohara-Nemoto, Y., Tajika, S., Sasaki, M. and Kimura, S.
  • Total Pages: 275-276
  • Publisher: Springer
• [Remarks]
  • URL: http://hitech-d.iwate-med.ac.jp/micr/index.htm