2011 Fiscal Year Final Research Report
Mechanism of EBV induced salivary gland disorder via TLR
| Project/Area Number |
21592347
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
INOUE Hiroko 鶴見大学, 歯学部, 准教授 (50367306)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Ichiro 鶴見大学, 歯学部, 教授 (60147634)
MISHIMA Kenji 昭和大学, 歯学部, 教授 (50275343)
RYO Kofuchi 鶴見大学, 歯学部, 講師 (10298268)
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| Project Period (FY) |
2009 – 2011
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| Keywords | EBウイルス / シェーグレン症候群 / TLR |
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| Research Abstract |
The salivary gland epithelial cells, HSY cells, were transfected with CD21-HA plasmid. EBV positive-B cell line, Akata, was cocultured with CD21-HSY cells for establishment of EBV positive salivary gland epithelial cells. The expression plasmids, TLR3, TLR8, and TLR9-HA, were prepared. To study of BAFF transcription, BAFF promoter region was flanked with luciferase coding region, BAFF-Luc. BALB/c mice were immunized with nuclear extract from Akata, and will be evaluated with ELISA for SSB/La, which was established with recombinant SSB/La-GST protein.
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[Journal Article] The role of fractalkine as accelerating factor on the autoimmune exocrinopathy in mice.2009
Author(s)
Tsubota, K., T. Nishiyama, K. Mishima, H. Inoue, T. Doi, Y. Hattori, T. Kodama, A. Higuchi, Y. Hayashi, and I. Saito
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Journal Title
Invest Ophthalmol Vis Sci
Volume: 50
Pages: 4753-4760
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