• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2011 Fiscal Year Final Research Report

Investigation of the secretion system of pathogenic proteinases in Porphyromonas gingivalis and study of the way to control the system

Research Project

  • PDF
Project/Area Number 21592364
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionNagasaki University

Principal Investigator

TSUKUBA Tomoko (KADOWAKI Tomoko)  長崎大学, 大学院・医歯薬学総合研究科, 助教 (70336080)

Co-Investigator(Renkei-kenkyūsha) NAKAYAMA Koji  長崎大学, 大学院・医歯薬学総合研究科, 教授 (80150473)
Project Period (FY) 2009 – 2011
Keywords細菌 / 歯周病 / 輸送・分泌 / 二成分制御系 / プロテアーゼ
Research Abstract

We investigated the secretion system of pathogenic proteinases/ adhesins in periodontopathogen Porphyromonas gingivalis. The secretion depends on a protein secretion system, Por secretion system(PorSS). We isolated several gingipain secretion-deficient mutants and found that two of them were deficient in a two-component system, which is a prokaryotic signal transduction system. PorY is a sensor histidine kinase and PorX acts as a response regulator. The recombinant PorY was autophosphorylated and the phosphoryl group on PorY was transferred to PorX. The interaction between PorY and PorX was also revealed by Surface plasmon resonance analysis. Gene expression of component proteins of the PorSS was also regulated by one of ECF sigma factors, PGN0274, independently of PorY-PorX system, suggesting that the secretion system is regulated by multiple regulatory mechanisms.

  • Research Products

    (9 results)

All 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Por secretion system-dependent secretion and glycosylation of Porphyromonas gingivalis hemin-binding protein 352011

    • Author(s)
      Shoji M, Sato K, Yukitake H, Kondo Y, Narita Y, Kadowaki T, Naito M, and Nakayama K
    • Journal Title

      PLoS One

      Volume: 6 Pages: e21372

    • Peer Reviewed
  • [Journal Article] Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages2009

    • Author(s)
      Tsukuba T, Yanagawa M, Okamoto K, Okamoto Y, Yasuda Y, Nakayama KI, Kadowaki T, Yamamoto K
    • Journal Title

      J Biochem

      Volume: 145(5) Pages: 565-573

  • [Presentation] Porphyromonas gingivalis HBP35タンパク質の菌体表面局在化機構の解析2011

    • Author(s)
      庄子幹郎、佐藤啓子、雪竹英治、近藤好夫、成田由香、門脇知子、内藤真理子、中山浩次
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      於長良川国際会議場
    • Year and Date
      2011-10-01
  • [Presentation] Characterization of a porK orthologue mutant in Tannerella forsythia2010

    • Author(s)
      Yuka Narita, Mikio Shoji, Keiko Sato, Hideharu Yukitake, Tomoko Kadowaki, Mariko Naito1, Yukitaka Murakami Fuminobu Yoshimura, Koji Nakayama
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      於パシフィコ横浜
    • Year and Date
      20100800
  • [Presentation] Role of the C-terminal domain in a novel secretion system in Porphyromonas gingivalis2010

    • Author(s)
      Mikio Shoji, Keiko Sato, Yuka Narita, Hideharu Yukitake, Tomoko Kadowaki, Mariko Naito, Koji Nakayama
    • Organizer
      Role of the C-terminal domain in a novel secretion system in Porphyromonas gingivalis第83回日本細菌学会総会
    • Place of Presentation
      於パシフィコ横浜
    • Year and Date
      20100800
  • [Presentation] 抗菌物質非鉄金属ポルフィリンの取り込みに関与するP. gingivalis外膜タンパク質2010

    • Author(s)
      雪竹英治、佐藤啓子、庄子幹郎、筑波(門脇)知子、内藤真理子、中山浩次
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      於タワーホール船堀
    • Year and Date
      2010-09-22
  • [Presentation] カテプシンE欠損マクロファージにおけるオートファジーの低下とそれに伴うミトコンドリア機能異常と酸化ストレスの上昇2009

    • Author(s)
      筑波隆幸、門脇知子、坂井詠子、岡元邦彰、山本健二
    • Organizer
      第51回歯科基礎医学会総会
    • Place of Presentation
      於新潟
    • Year and Date
      20090900
  • [Presentation] カテプシンE欠損によるオートファジー低下とそれに伴うミトコンドリア機能低下と酸化ストレスの増大2009

    • Author(s)
      筑波隆幸、柳川三千代、門脇知子、岡本美子、坂井詠子、岡元邦彰、山本健二
    • Organizer
      第14回日本病態プロテアーゼ学会
    • Place of Presentation
      於大阪
    • Year and Date
      20090800
  • [Remarks] ホームページ(長崎大学大学院医歯薬学総合研究科口腔病原微生物学分野)

    • URL

      http://www.med.nagasaki-u.ac.jp/mmi/ob/

URL: 

Published: 2013-07-31  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi