2011 Fiscal Year Final Research Report
Analysis of regulatory mechanism of bone destruction and inflammation in experimental periodontitis mediated by IL-27 receptor
| Project/Area Number |
21592366
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Saga University |
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Principal Investigator |
KUKITA Akiko 佐賀大学, 医学部, 准教授 (30153266)
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| Co-Investigator(Kenkyū-buntansha) |
KUKITA Toshio 九州大学, 大学院・歯学研究院, 教授 (70150464)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Hiroki 佐賀大学, 医学部, 教授 (40260715)
NAKAYAMA Koji 長崎大学, 医歯薬総合研究科, 教授 (80150473)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 破骨細胞 / 歯周病 / 細菌感染 / 骨破壊 / サイトカイン |
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| Research Abstract |
WSX-1 gene is a subunit of receptor for IL-27 which is involved in the regulation of immunity and inflammation. We analyzed the role of IL-27 in bone destruction of periodontitis using WSX-1 knocked-out mice. Mice were infected with Porphyromonas gingivalis which is one of major pathogens in periodontitis. Alveolar bone loss following P. gingivalis was enhanced in WSX-1 KO mice compared to those in wild-type mice. CD4-positive T cells in spleen of periodontitis mice produced higher amount of IL-17, and the production of IFN-γand IL-17 was further promoted in WSX-1 KO mice. P. gingivalis infection promoted differentiation from macrophages into osteoclasts, and osteoclast formation induced by infection was enhanced in macrophage of WSX-1 KO mice. The data suggest that signals through IL-27 receptor are involved in bone loss of periodontitis by affecting osteoclastogenesis directly and indirectly.
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