2011 Fiscal Year Final Research Report
Establishment of tooth regeneration using spheroid culture of neural crest-derived cells in bone marrow
| Project/Area Number |
21592373
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Yoichi 昭和大学, 歯学部, 准教授 (20295132)
TAKAMI Masamichi 昭和大学, 歯学部, 講師 (80307058)
MAYADA Atsushi 昭和大学, 歯学部, 講師 (50407558)
NAKAMURA Masanori 昭和大学, 歯学部, 教授 (50180394)
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| Co-Investigator(Renkei-kenkyūsha) |
OSUMI Noriko 東北大学, 医学系・研究科, 教授 (00220343)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 神経堤細胞 / 毛包 / 口腔粘膜 / 鼻甲介 / 象牙芽細胞 / 骨芽細胞 / 歯胚再生 |
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| Research Abstract |
Neural crest(NC) cells migrate ventro-laterally and populate the branchial arches during vertebrate development. Some NC cells are maintained in an undifferentiated state as NC-derived cells(NCDCs) throughout the life of the animal. We utilized P0-Cre/floxed-EGFP mice(P0 mice), in which NCDCs remain labeled with green fluorescent protein(GFP) even after birth. We observed the distribution of NCDCs in adult P0 mice and isolated them from other cells to form high concentrations of these cells. GFP-positive NCDC cells were found in tissues of the whisker pad, oral mucosa, and nasal conchae of the adult P0 mice. When isolated from other cells in these tissues, they were shown to form spheres under low-adherent conditions. Our findings demonstrate that GFP-positive NCDCs have the potential to differentiate into odontoblast-like cells or osteoblast-like cells. NCDCs from the whisker pad or oral mucosa, which are easily accessible in the oral and maxillofacial regions, can be cultured and may be a useful cell source for regeneration of these regions.
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