2011 Fiscal Year Final Research Report
Fundamental Evaluation of taste dysfunction in the aging society as a New Rapid Diagnostic Method using Carbonic Anhydrase VI in Parotid Saliva
| Project/Area Number |
21592465
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 味覚 / 唾液 / イムノクロマト法 / ELISA法 / 炭酸脱水酵素 |
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| Research Abstract |
It is known that more than 70% of patients with taste dysfunction have zinc deficiency. Carbonic anhydrase(CA) VI, zinc metallo-protein found in human saliva and is involved in taste dysfunction. Therefore, we developed a new quick and simple method of diagnosis using an immunochromatography assay to be used as an on-site test in substitution for a blood test. First, reactivity with a serum antibody was evaluated and compared to a control and detected with colloidal gold. A difference in color development was observed between samples of PBS(-) and the positive control(synthetic peptide). Furthermore, it was density dependent, and in serially diluted parotid gland saliva. The results suggest that an immunochromatography assay using this novel antibody can be used to quantitatively measure CA VI, which may be useful in the diagnosis of taste dysfunction caused by zinc deficiency.
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