Development of the cell-responsive DDS carrier suitable for bone formation and anti-cancer effects

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592489
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hokkaido University
• Principal Investigator: KASHIWAZAKI Haruhiko 北海道大学, 大学院・歯学研究科, 助教 (10344516)
• Co-Investigator(Kenkyū-buntansha): HAMADA Junichi 北海道大学, 遺伝子病制御研究所, 准教授 (50192703)
• Project Period (FY): 2009 – 2011
• Keywords: 顎骨再生 / DDS担体 / 歯学

Research Abstract

The objective of this study was to develop chitosan/hydroxyapatite(HAp) composite suitable for jaw regeneration, develop DDS technology for growth factors, and estimate bone formation and anti-cancer effects of the composites. By using the co-precipitation and porogen-leaching method, we developed a novel chitosan/HAp composite with various porosities, which was found to be mechanically flexible and easily formed into any desired shape. The composites with rhBMP-2 resulted in active bone formation. These results suggested that the composite was an effective material as a DDS carrier. Misexpressions of PRL-3 genes are implicated in the development of oral SCC. We hypothesized that the misexpression of HOX genes was associated with carcinogenesis and malignant progression. We plan to develop DDS materials which will act in the response with HOX genes expressions.

Research Products

• [Journal Article] ラット頭頂骨骨膜下におけるrhBMP-2添加多孔性キトサン/ハイドロキシアパタイト複合体による骨形成 (2012)
  • Author(s): 原田尚樹, 柏崎晴彦, 赤澤敏之, 村田勝, 相沢智康, 出村誠, 田中順三, 飯塚正, 井上農夫男
  • Journal Title: 北海道歯誌 Volume: 32 Pages: 166-176
  • Peer Reviewed
• [Journal Article] In vivo Evaluation of a Novel Chitosan/HAp Composite Biomaterial as a Carrier of rhBMP-2. (2010)
  • Author(s): Kashiwazaki H, Yamaguchi K, Harada N, Akazawa T, Murata M, Iizuka T, Ikoma T, Tanaka J, Inoue N
  • Journal Title: J. Hard Tissue Biology Volume: 19(3) Pages: 181-186
  • Peer Reviewed
• [Journal Article] In vitro and in vivo characteristics of chitosan fiber modified by an alternate soaking process (2009)
  • Author(s): Yoshioka T, Onomoto H, Kashiwazaki H, Inoue N, Koyama Y, Takakuda K, Tanaka J.
  • Journal Title: Mater. Trans Volume: 50(6) Pages: 1269-1272
  • Peer Reviewed
• [Journal Article] Fabrication of porous chitosan/hydroxyapatite nanocomposites : their mechanical and biological properties (2009)
  • Author(s): Kashiwazaki H, Kishiya Y, Matsuda A, Yamaguchi K, Iizuka T, Tanaka J, Inoue N
  • Journal Title: Biomed. Mater. Eng Volume: 19(2) Pages: 133-140
  • Peer Reviewed
• [Journal Article] Gene Expression Profile Changes Correlated with Lymph Node Metastasis in Oral Squamous Cell Carcinoma (2008)
  • Author(s): Kashiwazaki H, Hassan NMM, Hamada J, Moriuchi T, Yamazaki Y, Tei K, Totsuka Y, Inoue N
  • Journal Title: Odontology Volume: 96 Pages: 38-43
  • Peer Reviewed
• [Journal Article] PAX4 has the potential to function as a tumor suppressor in human melanoma (2008)
  • Author(s): Hata, S., Hamada, J., Maeda, K., Murai, T., Tada, M., Furukawa, H., Tsutsumida, A., Saito, A., Yamamoto Y. and Moriuchi, T
  • Journal Title: Int. J. Oncol Volume: 33 Pages: 1065-1071
  • Peer Reviewed
• [Presentation] キトサン/HAp複合体の骨形成および吸収性に関する検討 (2011)
  • Author(s): 柏崎晴彦,原田尚樹,赤澤敏之,村田勝,飯塚正,生駒俊之,田中順三,井上農夫男
  • Organizer: 第23回北海道骨粗鬆症研究会
  • Place of Presentation: 札幌市教育文化会館(札幌市)
  • Year and Date: 2011-01-15
• [Presentation] 多孔性キトサン/HAp複合体の機能設計とラット頭頂骨骨膜下の骨伝導 (2010)
  • Author(s): 原田尚樹,柏崎晴彦,飯塚正,村田勝,赤澤敏之,田中順三,井上農夫男
  • Organizer: 無機リン学会
  • Place of Presentation: 京都国際会議場(京都市)
  • Year and Date: 2010-10-07
• [Presentation] Bone augmentation by onlay implant using porous chitosan/HAp nanocomposites with rhBMP-2 on rat parietal bone (2009)
  • Author(s): Kashiwazaki H, Harada N
  • Organizer: 3rd Hiroshima Conference on Education and Science in Dentistry
  • Place of Presentation: Hiroshima
  • Year and Date: 20091107-08