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2011 Fiscal Year Final Research Report

Developments of scaffolds and processing technology to maximize the osteogenic potential of cultured periosteal sheets

Research Project

  • PDF
Project/Area Number 21592492
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dental engineering/Regenerative dentistry
Research InstitutionNiigata University

Principal Investigator

KAWASE Tomoyuki  新潟大学, 医歯学系, 准教授 (90191999)

Co-Investigator(Kenkyū-buntansha) OKUDA Kazuhiro  新潟大学, 医歯学系, 准教授 (00169228)
Project Period (FY) 2009 – 2011
Keywords培養骨膜シート / 骨再生 / 細胞基材 / 細胞プロセッシング
Research Abstract

We aimed to develop the fast processing methodology and novel scaffolds for human periosteal sheets. In general, scaffolds made of synthetic biodegradable polymers are not suitable for cell adhesion. However, our porous PLLA scaffold was capable of well securing periosteal sheets. This scaffold also functioned to facilitate extracellular matrices and cell penetration into deep pore regions. In this project, we have successfully developed several promising scaffolds with biodegradable polymermaterials.

  • Research Products

    (51 results)

All 2012 2011 2010 2009

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (28 results) Book (2 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 2 results)

  • [Journal Article] Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material2012

    • Author(s)
      Nakajima Y, Kawase T, Okuda K, Wolff LF, Yoshie H
    • Journal Title

      Platelets

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] A short-term preservation of human cultured periosteal sheets, osteogenic grafting materials, using a comer-cial preservation solution containing epigallocatechin-3-gallate(Thelio-keepR) under chilled conditions2012

    • Author(s)
      Kamiya M, Kawase T, Kobayashi M, Sekine Y, Okuda K, Nagata M, Fuse I, Nakata K, Wolff LF, Yoshie H
    • Journal Title

      Biopreserv Biobank

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] (総説)培養骨膜シート移植を応用した歯周組織再生法2012

    • Author(s)
      奥田一博、川瀬知之、中田光、吉江弘正
    • Journal Title

      再生医療(日本再生医療学会雑誌)

      Volume: 11(1) Pages: 51-56

    • Peer Reviewed
  • [Journal Article] A clinical study of the alveolar bone tissue engineering with cultured autogenous periosteal cells : coordinated activation of bone forma-tion and resorption2012

    • Author(s)
      Nagata M, Hoshina H, Li M, Arasawa M, Uematsu K, Ogawa S, Yamada K, Kawase T, Suzuki K, Ogose A, Amizuka N, Fuse I, Okuda K, Uoshima K, Nakata K, Yoshie H, Takagi R
    • Journal Title

      Bone

      Volume: 50(5) Pages: 1123-1129

    • Peer Reviewed
  • [Journal Article] An osteogenic grafting complex composed of human periosteal sheet and a porous poly(L-lactic acid) membrane scaffold : Biocompatibility, bio-degradability, and cell fate in vivo2012

    • Author(s)
      Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
    • Journal Title

      J Bioact Compat Polym

      Volume: 27(2) Pages: 107-121

    • Peer Reviewed
  • [Journal Article] Improved adhesion of human cultured periosteal sheets to a porous poly(L-lactic acid) membrane scaffold without the aid of exogenous adhesion biomolecules2011

    • Author(s)
      Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
    • Journal Title

      J Biomed Mater Res A

      Volume: 98(7) Pages: 100-113

    • Peer Reviewed
  • [Journal Article] Nondestructive microstructural analysis of porous bioceramics by microfocus X-ray computed tomography(μCT): A proposed protocol for standardized evaluation of porosity and interconnectivity between macro-pores2011

    • Author(s)
      Nakayama H, Burns DM, Kawase T
    • Journal Title

      J Nondest Eval

      Volume: 30(2) Pages: 71-80

    • Peer Reviewed
  • [Journal Article] Manual cryopreservation of human alveolar periosteal tissue segments : Effects of pre-culture on recovery rate2011

    • Author(s)
      Kawase T, Kogami H, Nagata M, Uematsu K, Okuda K, Burns DM, Yoshie H
    • Journal Title

      Cryobiol

      Volume: 62(3) Pages: 202-209

    • Peer Reviewed
  • [Journal Article] In-vivo near-infrared optical imaging of growing osteosar-coma cell lesions xenografted in mice : Dual-channel quantitative evaluation of volume and mineralization2011

    • Author(s)
      Nakayama H, Kawase T, Okuda K, Wolff LF, Yoshie H
    • Journal Title

      Acta Radiol

      Volume: 52(9) Pages: 978-988

    • Peer Reviewed
  • [Journal Article] Colla-gen-coated poly(L-lactide-co-ε-caprolactone) film : A promising scaffold for cultured periosteal sheets2010

    • Author(s)
      Kawase T, Yamanaka K, Suda Y, Kaneko T, Okuda K, Kogami H, Nakayama H, Nagata M, Wolff LF, Yoshie H
    • Journal Title

      J Periodontol

      Volume: 81 Pages: 1653-1662

    • Peer Reviewed
  • [Journal Article] (総説)歯周組織再生を促す生理活性物質と細胞療法に関するトランスレーショナルリサーチ2010

    • Author(s)
      川瀬知之
    • Journal Title

      日本歯周病学会誌

      Volume: 52(1) Pages: 3-11

    • Peer Reviewed
  • [Journal Article] Human periosteum-derived cells combined with superporous hydroxyl-apatite blocks used as an osteogenic bone substitute for periodontal regenerative therapy : Animal implant-tation study using nude mice2010

    • Author(s)
      Kawase T, Okuda K, Kogami H, Nakayama H, Nagata M, Sato T, Wolff LF, Yoshie H
    • Journal Title

      J Periodontol

      Volume: 81(3) Pages: 420-427

    • Peer Reviewed
  • [Journal Article] Osteogenic activity of human periosteal sheets cultured on salmon collagen-coated ePTFE meshes2010

    • Author(s)
      Kawase T, Okuda K, Kogami H, Nakayama H, Nagata M, Yoshie H
    • Journal Title

      J Mater Sci Mater Med

      Volume: 21(2) Pages: 731-739

    • Peer Reviewed
  • [Journal Article] Evaluation by bone scinti-graphy of osteogenic activity of commercial bioceramics(porousβ-TCP and HAp particles) subcutane-ously implanted in rats2010

    • Author(s)
      Nakayama H, Kawase T, Okuda K, Kogami H, Inoue H, Oda T, Hayama K, Tsuchimo-chi M, Wolff LW
    • Journal Title

      J Biomater Appl

      Volume: 24(8) Pages: 751-768

    • Peer Reviewed
  • [Journal Article] Characterization of human cultured periosteal sheets expressing bone-forming potential : in vitro and in vivo animal studies2009

    • Author(s)
      Kawase T, Okuda K, Kogami H, Nakayama H, Nagata M, Nakata K, Yoshie H
    • Journal Title

      J Tissue Eng Reg Med

      Volume: 3 Pages: 218-229

    • Peer Reviewed
  • [Journal Article] (総説)歯肉細胞シート・骨膜シートを用いた歯周再生治療2009

    • Author(s)
      吉江弘正、奥田一博、川瀬知之
    • Journal Title

      日本口腔外科学会雑誌

      Volume: 55(9) Pages: 432-439

    • Peer Reviewed
  • [Journal Article] X線マイクロCTによる生体活性セラミックス多孔体の微小構造解析2009

    • Author(s)
      中山均、川瀬知之
    • Journal Title

      歯科放射線

      Volume: 49(3) Pages: 33-40

    • Peer Reviewed
  • [Presentation] Tissue engineered cultured periosteal sheet application to periodontal regeneration : three year results2011

    • Author(s)
      Okuda K, Kawase T, Nagata M, Nakata K, Wolff LF, Yoshie H
    • Organizer
      The 97th Annual Meeting of the Amer Acad Periodontol
    • Place of Presentation
      Miami Beach, FL, USA
    • Year and Date
      20111112-15
  • [Presentation] Freeze-dried platelet-rich plasma(PRP)-coated polyglactin mesh : a promising alternative for wound care and periodontal regenerative therapy2011

    • Author(s)
      Kawase T, Nakajima Y, Okuda K, Wolff LF, Yoshie H
    • Organizer
      The 97th Annual Meeting of the Amer Acad Periodontol
    • Place of Presentation
      Miami Beach, FL, USA
    • Year and Date
      20111112-15
  • [Presentation] 生体吸収性ポリエステル多孔質膜を足場とする骨膜細胞シートの培養2011

    • Author(s)
      多田晋一郎、高屋朋彰、川瀬知之、田中孝明
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋工業大学、名古屋
    • Year and Date
      20110914-16
  • [Presentation] A wound-dressing coated with freeze-dried platelet-rich plasma : Its potent positive effects on wound-healing2011

    • Author(s)
      Nakajima Y, Kawase T, Okuda K, Wolff LF, Yoshie H
    • Organizer
      Tissue Eng Regen Med Int Soc-Asia Pacific Chap 2011
    • Place of Presentation
      Singapore, Singapore
    • Year and Date
      20110803-05
  • [Presentation] Angiogenic effects of human platelet-rich fibrin(PRF) in chicken chorioallantoic membranes2011

    • Author(s)
      Kobayashi M, Kawase T, Okuda K, Wolff LF, Yoshie H
    • Organizer
      Tissue Eng Regen Med Int Soc-Asia Pacific Chap 2011
    • Place of Presentation
      Singapore, Singapore
    • Year and Date
      20110803-05
  • [Presentation] An osteogenic grafting complex composed of human periosteal sheet and its scaffolding material, a porous poly(L-lactic acid) membrane : biocompatibility, biodegradation, and cell fate in vivo2011

    • Author(s)
      Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
    • Organizer
      Tissue Eng Regen Med Int Soc-Asia Pacific Chap 2011
    • Place of Presentation
      Singapore, Singapore
    • Year and Date
      20110803-05
  • [Presentation] 培養骨膜シートを用いた歯周組織再生療法の3年予後2011

    • Author(s)
      奥田一博、川瀬知之、山宮かの子、吉江弘正
    • Organizer
      第54回春季日本歯周病学会学術大会
    • Place of Presentation
      福岡歯科大、福岡
    • Year and Date
      20110527-28
  • [Presentation] 治療用ヒト培養骨膜シートの凍結保存法2011

    • Author(s)
      川瀬知之、小神浩幸、永田昌毅、上松晃也、奥田一博、吉江弘正
    • Organizer
      第54回春季日本歯周病学会学術大会
    • Place of Presentation
      福岡歯科大、福岡
    • Year and Date
      20110527-28
  • [Presentation] 骨膜シートによる歯周組織・顎骨の再生療法2011

    • Author(s)
      吉江弘正、奥田一博、川瀬知之、永田昌毅
    • Organizer
      シンポジウム「再生と再建」第28回日本医学会総会
    • Place of Presentation
      東京
    • Year and Date
      20110408-10
  • [Presentation] 骨再生のための生体吸収性ポリマーブレンド多孔質膜の作製および特性評価2011

    • Author(s)
      高屋朋彰、川瀬知之、田中孝明
    • Organizer
      化学工学会第76年会
    • Place of Presentation
      東京農工大、東京
    • Year and Date
      20110322-24
  • [Presentation] 培養骨膜シートを用いた歯周組織再生療法の3年予後2011

    • Author(s)
      奥田一博、川瀬知之、梶昌美、中田光、吉江弘正
    • Organizer
      第10回日本再生医療学会
    • Place of Presentation
      東京女子医大、東京
    • Year and Date
      20110301-02
  • [Presentation] 骨膜シートによる歯周組織・顎骨の再生療法.シンポジウム「歯周組織はどこまで再生するか」2011

    • Author(s)
      吉江弘正、奥田一博、川瀬知之、永田昌毅、中田光
    • Organizer
      第10回日本再生医療学会
    • Place of Presentation
      東京女子医大、東京
    • Year and Date
      20110301-02
  • [Presentation] 培養骨膜シートと基材の複合化による厚みのある骨原性移植物の開発.シンポジウム「細胞3次元構築を目指した組織工学」2011

    • Author(s)
      川瀬知之
    • Organizer
      第10回日本再生医療学会
    • Place of Presentation
      東京女子医大、東京
    • Year and Date
      20110301-02
  • [Presentation] 幹細胞用培地は骨膜シートのポテンシャル向上に貢献するか?2011

    • Author(s)
      上松晃也、川瀬知之、永田昌毅、奥田一博、吉江弘正、高木律夫
    • Organizer
      第54回秋季日本歯周病学会学術大会
    • Place of Presentation
      広島大、下関
    • Year and Date
      2011-09-24
  • [Presentation] Clinical and histologic evaluation of tissue-engineered cultured periosteum application for bone regeneration2010

    • Author(s)
      Okuda K, Yoshie H, Kawase T, Kogami H, Nakayama H, Nagata M, Takagi R
    • Organizer
      The 96th Annual Meeting of the Amer Acad Periodontol
    • Place of Presentation
      Honolulu, HI, USA
    • Year and Date
      20101030-1102
  • [Presentation] Non-invasive evaluation of the osteogenic activity of alveolar bone-derived human periosteal sheets in animal implantation models by in vivo near-infrared(NIR) fluorescence optical imaging2010

    • Author(s)
      Kawase T, Nakayama H, Okuda K, Kogami H, Nagata M, Wolff LF, Yoshie H
    • Organizer
      The 96th Annual Meeting of the Amer Acad Periodontol
    • Place of Presentation
      Honolulu, HI, USA
    • Year and Date
      20101030-1102
  • [Presentation] 培養細胞による非晶質水酸アパタイト骨充填材の製造法2010

    • Author(s)
      川瀬知之
    • Organizer
      イノベーション・ジャパン2010-大学見本市
    • Place of Presentation
      JST, NEDO、東京
    • Year and Date
      20100929-1001
  • [Presentation] A porous poly(L-lactic acid) membrane designed for culturing human perios-teal sheets as an osteogenic grafting material2010

    • Author(s)
      Kawase T, Tanaka T, Nishimoto T, Okuda K, Nagata M, Burns DM, Yoshie H
    • Organizer
      Tissue Eng Regen Med Int Soc-Asia Pacific Chap 2010
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      20100915-17
  • [Presentation] 骨膜片採取から移植治療用培養骨膜シート形成に至る過程での細胞動態の分析2010

    • Author(s)
      奥田一博、川瀬知之、小神浩幸、永田昌毅、吉江弘正
    • Organizer
      第53回春季日本歯周病学会学術大会
    • Place of Presentation
      岩手医大、盛岡
    • Year and Date
      20100513-15
  • [Presentation] 歯周組織再生を促す生理活性物質と細胞療法に関するトランスレーショナルリサーチ2010

    • Author(s)
      川瀬知之
    • Place of Presentation
      岩手医大、盛岡
    • Year and Date
      20100513-15
  • [Presentation] 異所性骨形成モデルにおけるNIR(近赤外線)検査の応用の試み2010

    • Author(s)
      中山均、川瀬知之、小神浩幸、奥田一博、吉江弘正
    • Organizer
      第9回日本再生医療学会
    • Place of Presentation
      広島大、広島
    • Year and Date
      20100318-19
  • [Presentation] 培養自家骨膜シートをもちいた歯周組織再生療法に関する基礎的研究.シンポジウム「歯科領域の再生医療」2010

    • Author(s)
      川瀬知之
    • Organizer
      第9回日本再生医療学会
    • Place of Presentation
      広島大、広島
    • Year and Date
      20100318-19
  • [Presentation] 多血小板フィブリン(PRF)の創傷治癒に及ぼす効果2010

    • Author(s)
      中島悠、川瀬知之、奥田一博、吉江弘正
    • Organizer
      第53回秋季日本歯周病学会学術大会
    • Place of Presentation
      徳島大、高松
    • Year and Date
      2010-09-19
  • [Presentation] 創傷治癒・組織再生に有効な生体活性成分を含む基材2010

    • Author(s)
      川瀬知之
    • Organizer
      新技術説明会
    • Place of Presentation
      新潟大学、東京
    • Year and Date
      2010-07-30
  • [Presentation] The human cultured periosteal sheet for peri-odontal regeneration : A salmon collagen-coated mesh, a functional potent scaffold, to upregulate the osteogenic potential2009

    • Author(s)
      Okuda K, Yoshie H, Kawase T, Kogami H, Nakayama H, Nagata M
    • Organizer
      The 95th Annual Meeting of the Amer Acad Periodontol
    • Place of Presentation
      Boston, MA, USA
    • Year and Date
      20090912-15
  • [Presentation] 歯周組織再生を促す生理活性物質と細胞療法に関するトランスレーショナルリサーチ2009

    • Author(s)
      川瀬知之
    • Organizer
      第52回日本歯周病学会秋季学術大会
    • Place of Presentation
      九州大、宮崎
    • Year and Date
      2009-10-11
  • [Presentation] インプラント症例を対象とした培養自家骨膜による歯槽骨再生2009

    • Author(s)
      永田昌毅、川瀬知之、吉江弘正、奥田一博、中田光、高木律男
    • Organizer
      第52回日本歯周病学会秋季学術大会
    • Place of Presentation
      九州大、宮崎
    • Year and Date
      2009-10-11
  • [Presentation] 歯科インプラントを目的とした培養自家骨膜併用による歯槽骨再生2009

    • Author(s)
      永田昌毅、星名秀行、川瀬知之、荒澤恵、山田一穂、嵐山貴徳、中田光、高木律男
    • Organizer
      日本形成外科学会関東支部第80回新潟地方会
    • Place of Presentation
      新潟大歯、新潟
    • Year and Date
      2009-07-13
  • [Book] 凍結乾燥PRPコート創傷被覆材の開発.第6章医薬品応用,『化粧品・医薬品における次世代経皮吸収製剤』2011

    • Author(s)
      川瀬知之,杉林堅次
    • Total Pages
      214-223
    • Publisher
      株式会社シーエムシー
  • [Book] 第3章細胞の利用2.前駆細胞、幹細胞10)培養骨膜シート移植を応用した歯周組織再生治療.遺伝子医学MOOK13患者までとどいている再生誘導治療-バイオマテリアル、生体シグナル因子、細胞を利用した患者のための再生医療の実際-2009

    • Author(s)
      奥田一博、川瀬知之、山宮かの子、吉江弘正、田畑泰彦
    • Total Pages
      225-229
    • Publisher
      株式会社メディカルドゥ
  • [Patent(Industrial Property Rights)] フィブリンゲル圧延装置2012

    • Inventor(s)
      山内慶次郎、阿部和幸、川瀬知之
    • Industrial Property Rights Holder
      YSEC株式会社、新潟大学
    • Industrial Property Number
      特許、PCT/JP2012/050545
    • Filing Date
      2012-01-13
    • Overseas
  • [Patent(Industrial Property Rights)] フィブリンゲル圧延装置2011

    • Inventor(s)
      山内慶次郎、阿部和幸、川瀬知之
    • Industrial Property Rights Holder
      YSEC株式会社、新潟大学
    • Industrial Property Number
      意匠、登録第1419943号
    • Acquisition Date
      2011-07-01
  • [Patent(Industrial Property Rights)] 培養細胞が産生する石灰化物による骨形成・再生2010

    • Inventor(s)
      川瀬知之
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Number
      特許、PCT/JP2010/53661
    • Filing Date
      2010-03-05
    • Overseas
  • [Patent(Industrial Property Rights)] 再生治療用材料2009

    • Inventor(s)
      川瀬知之、奥田一博、中島悠
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Number
      特許、特願2009-281382
    • Filing Date
      2009-12-11

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Published: 2013-07-31  

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