2011 Fiscal Year Final Research Report
Induction of tooth and craniofacial tissue from undifferentiated presumptive ectodermal Xenopus cell by aggregation culture method.
| Project/Area Number |
21592527
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
FUKUI Yasuto 広島大学, 大学院・医歯薬学総合研究科, 助教 (90363085)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Tetsuji 広島大学, 大学院・医歯薬学総合研究科, 教授 (00169153)
TORATANI Shigeaki 広島大学, 病院, 講師 (90172220)
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| Project Period (FY) |
2009 – 2011
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| Keywords | アニマルキャップ / 歯牙誘導 / 顎骨誘導 |
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| Research Abstract |
We used ectodermal Xenopus cell by aggregation culture method. Pas/ Alcian blue stained positive cartilage tissue was observed in the aggregate. Next, we implanted the aggregate into the abdominal region of Xenopus embryo. We observed PAS/ Alcian blue positive and Amelogenin positive tooth germ-like tissue in transplanted tissue after the host transformation. More, we implanted the aggregate to the embryo(St23) was exicised region of lower jaw, we were not able to observe Pas/ Alcian blue stained positive cartilage tissue.
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