2012 Fiscal Year Final Research Report

Study of SP cells under hypoxic conditions and molecular targeted therapy

Research Project

Project/Area Number	21592558
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Surgical dentistry
Research Institution	Nagasaki University
Principal Investigator	KAWASAKI Goro 長崎大学, 医歯薬学総合研究科, 准教授 (60195071)
Project Period (FY)	2009 – 2012
Keywords	SP細胞 / 低酸素環境 / 扁平上皮癌細胞
Research Abstract	Side population (SP) cells were isolated from oral cancer cell lines. SP cells possessed high malignant ability and demonstrated more drug resistance to 5-FU, as compared with non-SP cells. Under hypoxic conditions, HIF-1 expressions was associated with pathological malignancy grades, particularly activation of mTOR-HIF-1α-VEGF pathway was associated with pathological malignancy grades. Oral squamous cell carcinoma cell lines were treated with mTOR inhibitor, thereafter carcinoma cells revealed cell growth inhibition and reduced invasion. Under hypoxic conditions, HIF-1α was activated. The expression of mTOR-HIF-1α-VEGF pathway under hypoxic conditions was decreased by the treatment with mTOR inhibitor.