Effect of oral malodorous compound on the osteoclast differentiation and alveolar bone loss.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592667
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: IMAI Toshio 日本歯科大学, 生命歯学部, 准教授 (90120617)
• Co-Investigator(Kenkyū-buntansha): YAEGAKI Ken 日本歯科大学, 生命歯学部, 教授 (40166468) ; MURATA Takatoshi 日本歯科大学, 生命歯学部, 講師 (10313529)
• Project Period (FY): 2009 – 2011
• Keywords: 予防歯科学

Research Abstract

The objective of this study was to elucidate the signal transduction mechanisms of hydrogen sulfide-induced osteoclast differentiation in primary cultued osteoclast precursor cells and RAW264 cells. Hydrogen sulfide at physiologic concentrations in mouth air induced the differentiation of mouse bone marrow-derived osteoclast precursor cells in the absence of RANKL. Moreover, hydrogen sulfide activated ERK1/2 phosphorylation in the osteoclast precursor cells. A low concentrations of NaHS induced PKC phosphorylation in RAW 264 cells. When pretreatment with PKC inhibitor(GF109203x), NaHS-induced the activation of PKC, c-Raf and ERK1/2 in RAW264 cells were diminished. These results suggested that hydrogen sulfide at physiologic concentrations stimulate the osteoclast differentiation through the PKC, cRaf, ERK1/2 pathway.