Modulation of nociceptive genes by in vivo RNA interference in rats and application to pain treatment

2011 Fiscal Year Final Research Report

• Project/Area Number: 21600006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 疼痛学
• Research Institution: Nagasaki University
• Principal Investigator: TODA Kazuo 長崎大学, 医歯薬学総合研究科, 教授 (80134708)
• Co-Investigator(Kenkyū-buntansha): KIMOTO Mari 日本女子大学, 家政学部, 准教授 (60101565)
• Project Period (FY): 2009 – 2011
• Keywords: 眼窩下神経結紮モデル / 三叉神経脊髄路核 / トランスフェクション / RNA干渉 / フォンフライ毛 / 外界電位 / 逃避反射 / ラット

Research Abstract

Neuropathic pain can be very difficult to treat. It may be associated with abnormal sensations called dysesthesia, and pain is produced by normally non-noxious stimuli(allodynia). Recently, it is reported that nociceptive-genes are strongly concerned with producing neuropathic pain and inhibition of nocicptive-gene expression is one of the tools to treat neuropathic pain. This study revealed that inhibition of nocicptive-genes by in vivo RNA interference elevated threshold value to mechanical noxious stimuli.

Research Products

• [Journal Article] Hypergravity conditioning on ileal movements in rats (2012)
  • Author(s): Kimoto M, Zeredo JL, Toda K
  • Journal Title: Aviation, Space and Environmental Medicine Volume: (in press)
• [Journal Article] Irritant-drinking Behaviour can be Modifi ed by gravity-stress loaded in developing but not in adult rats (2011)
  • Author(s): Kimoto M, Zeredo JL, Toda K
  • Journal Title: Stress and Health Volume: 27 Pages: 34-41
• [Journal Article] Response properties of nucleus reticularis lateralis neurons after electroacupuncture stimulation in rats (2010)
  • Author(s): Moritaka K, Zeredo JL, Kimoto M, Nasution FH, Hirano T, Toda K
  • Journal Title: Am J Chin Med Volume: 38(5) Pages: 869-80
• [Presentation] Antinociceptive effects induced by gravity stress in rats : Sex differences (2011)
  • Author(s): Toda K, Zeredo JL, Moritaka K, Yamashita H, Kaida K, Kimoto M
  • Organizer: 43rd Meeting of the European Brain and Behavior Society(EBBS)
  • Place of Presentation: Seville, Spain
  • Year and Date: 20110909-12
• [Presentation] Comparison of stress-induced modulation of smooth muscle activities between small and large intestine in adult rats (2011)
  • Author(s): Kimoto M, Ikezaki M, Kato M, Kohata A, Takashima K, Takeuchi M, Nemoto S, Zeredo JL, Toda K
  • Organizer: 43rd Meeting of the European Brain and Behavior Society(EBBS)
  • Place of Presentation: Seville, Spain
  • Year and Date: 20110909-12
• [Presentation] Signaling pathway in extracellular calcium sensing of frog parathyroid cells (2011)
  • Author(s): Okada Y, Miyazaki T, Hotokezaka H, Fujiyama R, Toda K
  • Organizer: ICCPB2011
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20110531-0605
• [Presentation] Stress-induced modulation of ileal peristaltic movement in rats (2010)
  • Author(s): Kimoto M, Kumei Y, Zeredo JL, Toda K
  • Organizer: 7th World Congress on Stress, Leiden
  • Place of Presentation: The Netherlands
  • Year and Date: 20100825-27
• [Presentation] Trigeminal irritant-induced behavior can be modified by gravity stress in younger rats, EFIC-conferences (2009)
  • Author(s): Kimoto M, Zeredo JL, Kumei Y, Toda K
  • Organizer: Pain in Europe VI
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 20090909-12
• [Presentation] Nociceptive behaviors in rats at low gravity conditions, EFIC-conferences (2009)
  • Author(s): Ishida, T, Zeredo JL, Yabushita T, Seki S, Fusejima Y, Fukushima G, Toda K, Kumei Y
  • Organizer: Pain in Europe VI
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2009-09-12
• [Book] 基礎解剖生理学第3版 (2011)
  • Author(s): 戸田一雄,木本万里
  • Total Pages: 1-449
  • Publisher: おうふう出版
• [Book] はじめての痛み学 (2011)
  • Author(s): 植田弘師,戸田一雄
  • Total Pages: 1-170
  • Publisher: おうふう出版