2011 Fiscal Year Final Research Report
Comparative evolutionary studies on three-dimensionally positioning of the chromosome territories in the primate iPS cell nuclei
| Project/Area Number |
21602002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Stem cell biology and medical science
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| Research Institution | The Graduate University for Advanced Studies |
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Principal Investigator |
TANABE Hideyuki 総合研究大学院大学, 先導科学研究科, 准教授 (50261178)
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| Co-Investigator(Renkei-kenkyūsha) |
HIRAI Hirohisa 京都大学, 霊長類研究所, 教授 (10128308)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 染色体テリトリー / 3D-FISH法 / 核内配置 / 細胞分化 / 遺伝子ポジショニング / 霊長類iPS細胞 / ゲノム配置 |
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| Research Abstract |
In this study from the viewpoint of spatial positioning of chromosome territories and genomic regions within the nucleus I would try to find the molecular mechanism of why the iPS cells are established and to identify what the human specific characteristics have been appeared during the course of primate evolution by comparative analysis in human and great apes. Analysis by 3D-FISH technique using the DNA probes with Oct3/4, Sox2, Klf4, c-Myc, and Nanog, and their chromosomal paints onto human, chimpanzee, gorilla, and orangutan fibroblast cell nuclei, basic characteristics of spatial arrangements have been revealed but analysis has not yet been carried out due to shortage of primate iPS cells. When accumulating iPS cells, I would perform the analyses and discuss the issues.
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